Interpretation:
In the preparation of a TLC plate for the development, the result of making large spots needs to be explained.
Concept Introduction:
Chromatography is the best method of separation of components from the mixture as it gives most accurate results compare to other methods. In this method the mixture must be dissolved in certain fluid that is called as mobile phase. This mobile phase carries different components of mixture on the structure holding another material. This material is known as stationary phase. There are various types of chromatography methods such as column chromatography, thin layer chromatography, gas chromatography etc.
Answer to Problem 1Q
The large spot concludes that a lot of sample moves up in the plate that makes difficult to analyze the separation.
Explanation of Solution
In TLC technique or Thin layer chromatography, the solvent is used to separate the components of a mixture. The polarity of solvent plays an important role here. If the solvent has high polarity, all components will move along with it and cause large spot with no separation. Similarly if the solvent has low polarity, the components of mixture will not move that causes incomplete separation of them.
Hence large spot concludes that a lot of sample moves up in the plate that makes difficult to analyze the separation.
Thus, the large spot concludes that a lot of sample moves up in the plate that makes difficult to analyze the separation.
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Chapter 10 Solutions
EBK A SMALL SCALE APPROACH TO ORGANIC L
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- Which statement about TLC analyses is not true? * A- The line at the base of the TLC plate should be drawn with a pencil. B- To prevent contamination, a new capillary tube should be used to apply each sample. C- Samples should be applied multiple times in the same place to increase the concentrations of any possible compounds in the spot. D- A glass beaker lined with filter paper can be used as a development chamber in a TLC analysis. E- All these statements are correct.arrow_forwardWhat is a guard column and why is it used in HPLC but not in GC systems?arrow_forwardWhat is chiral chromatography?arrow_forward
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