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Concept explainers
Interpretation:
The manner in which the DNA polymerases of prokaryotes differ from those of eukaryotes is to be explained.
Concept introduction:
DNA (deoxyribonucleic acid) polymerase is the enzyme that catalyzes the process of duplication of DNA from the host template to the daughter template in the presence of deoxynucleotides.
The eukaryotes contain at least 19 different types of DNA polymerases, while the prokaryotes contain only 5 types of DNA polymerases.
Exonucleases are the enzymes that have the ability to remove the
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Chapter 10 Solutions
Bundle: Biochemistry, Loose-leaf Version, 9th + Owlv2,1 Term Printed Access Card
- REFLECT AND APPLY In the MeselsonStahl experiment that established the semiconservative nature of DNA replication, the extraction method produced short fragments of DNA. What sort of results might have been obtained with longer pieces of DNA?arrow_forwardREFLECT AND APPLY What is the complete base composition of a double-stranded eukaryotic DNA that contains 22% guanine?arrow_forwardREFLECT AND APPLY What difficulties arise in the polymerase chain reaction if there is contamination of the DNA that is to be copied?arrow_forward
- REFLECT AND APPLY Diagram a section of DNA being transcribed. Give the various names for the two strands of DNA.arrow_forwardREFLECT AND APPLY List three mechanisms that relax the twisting stress in helical DNA molecules.arrow_forwardREFLECT AND APPLY Suggest a reason why it would be unlikely for replication to take place without unwinding the DNA helix.arrow_forward
- REFLECT AND APPLY E. coli incorporates deoxyribonucleotides into DNA at a rate of 250 to 1000 bases per second. Using the higher value, translate this into typing speed in words per minute. (Assume five characters per word, using the typing analogy from Question 36.)arrow_forwardREFLECT AND APPLY Give the DNA sequence for the template strand that gives rise to the following sequence gel, prepared using the Sanger method with a radioactive label at the 5' end of the primer.arrow_forwardREFLECT AND APPLY Your book contains about 2 million characters (letters, spaces, and punctuation marks). If you could type with the accuracy with which the prokaryote E. coli incorporates, proofreads, and repairs bases in replication (about one uncorrected error in 109to1010 bases), how many such books would you have to type before an uncorrected error is permitted? (Assume that the error rate is one in 1010 bases.)arrow_forward
- REFLECT AND APPLY Suppose that you are a prosecuting attorney. How has the introduction of the polymerase chain reaction changed your job?arrow_forwardREFLECT AND APPLY Is the following statement true or false? Why? The flow of genetic information in the cell is always DNARNAprotein.arrow_forwardREFLECT AND APPLY How can breakdown in DNA repair play a role in the development of human cancers?arrow_forward
- BiochemistryBiochemistryISBN:9781305961135Author:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougalPublisher:Cengage Learning
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