Brock Biology of Microorgan. -Access
14th Edition
ISBN: 9780321943736
Author: MADIGAN
Publisher: PEARSON
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Chapter 10.6, Problem 1MQ
- During transformation a cell usually incorporates only one or a few fragments of DNA. Explain.
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During transformation a cell usually incorporates only one or afew fragments of DNA. Explain.
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Which DNA repair systems you think might be capable of repairing a situation where T is in one strand and G is in the complementary strand? Explain dramatically.
Chapter 10 Solutions
Brock Biology of Microorgan. -Access
Ch. 10.1 - Distinguish between a mutation and a mutant.Ch. 10.1 - Distinguish between screening and selection.Ch. 10.2 - Do missense mutations occur in genes encoding...Ch. 10.2 - Why do frameshift mutations generally have more...Ch. 10.3 - Why does the Ames test measure the rate of...Ch. 10.3 - Which class of mutation, missense or nonsense, is...Ch. 10.4 - Prob. 1MQCh. 10.4 - Prob. 2MQCh. 10.4 - Prob. 3MQCh. 10.5 - Prob. 1MQ
Ch. 10.5 - Prob. 2MQCh. 10.5 - Prob. 3MQCh. 10.6 - During transformation a cell usually incorporates...Ch. 10.6 - Prob. 2MQCh. 10.7 - Prob. 1MQCh. 10.7 - What is the major difference between generalized...Ch. 10.7 - Why is phage conversion considered beneficial to...Ch. 10.8 - In conjugation, how are donor and recipient cells...Ch. 10.8 - Explain how rolling circle DNA replication allows...Ch. 10.8 - Prob. 3MQCh. 10.9 - In conjugation involving the F plasmid of...Ch. 10.9 - Prob. 2MQCh. 10.9 - Prob. 3MQCh. 10.10 - Why is it usually more difficult to select...Ch. 10.10 - Why do penicillins not kill species of Archaea?Ch. 10.11 - Prob. 1MQCh. 10.11 - What is the significance of the terminal inverted...Ch. 10.11 - How can transposons be used in bacterial genetics?Ch. 10.12 - Why is the CRISPR system considered a prokaryotic...Ch. 10.12 - Prob. 2MQCh. 10 - Write a one-sentence definition of the term...Ch. 10 - Prob. 2RQCh. 10 - Prob. 3RQCh. 10 - Prob. 4RQCh. 10 - Prob. 5RQCh. 10 - What are heteroduplex regions of DNA and what...Ch. 10 - QExplain why recipient cells do not successfully...Ch. 10 - QExplain how a generalized transducing particle...Ch. 10 - QWhat is a sex pilus and which cell type, F or F+,...Ch. 10 - Prob. 10RQCh. 10 - Prob. 11RQCh. 10 - Prob. 12RQCh. 10 - QExplain why incoming DNA recognized by a short...Ch. 10 - A constitutive mutant is a strain that...Ch. 10 - Although a large number of mutagenic chemicals are...Ch. 10 - Why is it difficult in a single experiment to...Ch. 10 - Prob. 4AQ
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- DNA isolated from an organism can be sheared into fragments of uniform size (∼1000 bp), heated to separate the strands, then cooled to allow complementary strands to reanneal. The renaturation process can be followed over time. Explain why the renaturation of E. coli DNA is a monophasic process, whereas the renaturation of human DNA is biphasic (an initial rapid phase followed by a slower phase).arrow_forwardWhat increases transformation efficiency?arrow_forwardIn producing genetically engineered human insulin in bacteria, why is it important to use the samerestriction enzyme to cut both the human DNA and the bacterial plasmid?arrow_forward
- Explain why the absorption of UV light by double-stranded DNA increases (the hyperchromic effect) when the DNA is denatured.arrow_forwardAssume the sequence below is one half of a double stranded DNA template used in a PCR reaction. The highlighted sequences indicate the region bound by primers, either on this strand or on the other complementary strand. 5' ACGTGCGACACGTATATATGTCGCGTGAGTGTAGCGTATCGCTAGAGACGCATACCTATG 3' If the sequence of the forward primer is 5' GCGACACG 3', which of the following sequences would represent the reverse primer? a. 5’ – CAGAGATCGC – 3’ b. None of these sequences would represent the reverse primer c. 5’ – GTCTCTAGCG – 3’ d. 5’ – GCGATCTCTG – 3’ e. 5’ – CGCTAGAGAC – 3’arrow_forwardGiven the following coding sequence for DNA, provide the sequence of the complementary(template) sequence. 5’ ATGCATAGATTAGGATATCCCAGATAG 3’arrow_forward
- Restriction endonuclease digestion of a DNA sequence yielded fragments of the following sizes: 1. 5.2 kb 2. 0.8 kb 3. 1.2 kb 4. 3.8 kb 5. 3.1 kb After gel electrophoresis, what would be the order in which these fragments would be found—the last fragment listed being furthest from the negative pole.arrow_forwardWhich of these two changes is more difficult for DNA repair enzymes to fix correctly? Explain why.arrow_forwardProkaryotic DNA is normally slightly negatively supercoiled, which can lead to "DNA breathing". How or why does supercoiling lead to DNA "breathing"?arrow_forward
- In experiments using polymerase chain reactions (PCR), it is often more difficult to amplifythrough regions of DNA that are high in GC content versus those regions that are either lower inGC content or are AT-rich. Based on your knowledge of DNA structure, explain why.arrow_forwardDuring agarose gel electrophoresis, why does DNA move through the gel when electric current is applied? because DNA is negatively charged because a charged chemical from the loading buffer is bound to the DNA because DNA is positively charged because DNA absorbs electricityarrow_forwardWhy do DNA chips often contain segments derived from cDNA rather than genomic DNA segments?arrow_forward
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