GENETIC ANALYSIS: AN INTEG. APP. W/MAS
2nd Edition
ISBN: 9781323142790
Author: Sanders
Publisher: Pearson Custom Publishing
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Chapter 11, Problem 25P
The molecular probes used in FISH can detect repetitive DNA sequences or unique sequences that are parts of genes.
How are the binding locations of FISH probes on chromosomes identified?
Distinguish the detection of a FISH probe from the detection of a molecular probe in a Southern blot.
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The technique of fluorescence in situ hybridization (FISH) is described. This is another method for examining sequence complexity within a genome. In this method, a DNA sequence, such as a particular gene sequence, can be detected within an intact chromosome by using a DNA probe that is complementary to the sequence.For example, let’s consider the β-globin gene, which isfound on human chromosome 11. A probe complementary to theβ-globin gene binds to that gene and shows up as a brightly colored spot on human chromosome 11. In this way, researchers can detectwhere the β-globin gene is located within a set of chromosomes. Becausethe β-globin gene is unique and because human cells are diploid(i.e., have two copies of each chromosome), a FISH experimentshows two bright spots per cell; the probe binds to each copy ofchromosome 11. What would you expect to see if you used thefollowing types of probes?A. A probe complementary to the Alu sequenceB. A probe complementary to a tandem array near…
Chapter 11 Solutions
GENETIC ANALYSIS: AN INTEG. APP. W/MAS
Ch. 11 - Prob. 1PCh. 11 - Prob. 2PCh. 11 - Bacterial DNA is compacted by two principal...Ch. 11 - 10.2 The human genome contains contains base...Ch. 11 - 10.1 Give descriptions for the following...Ch. 11 - 10.4 Describe the importance of light and dark G...Ch. 11 - In eukaryotic DNA, Where are you most likely to...Ch. 11 - Prob. 8PCh. 11 - Human late prophase karyotypes have about 2000...Ch. 11 - 10. What are the two or three most essential...
Ch. 11 - Prob. 11PCh. 11 - Prob. 12PCh. 11 - A researcher interested in studying a human gene...Ch. 11 - Prob. 14PCh. 11 - 10.11 In what way does position effect variegation...Ch. 11 - 16. What are chromosome territories, and what...Ch. 11 - Prob. 17PCh. 11 - Prob. 18PCh. 11 - 10.18 A survey of organisms living deep in the...Ch. 11 - A eukaryote with a diploid number of 2n=6 carries...Ch. 11 - The accompanying chromosome diagram represents a...Ch. 11 - Suppose the genome of a bacterium contains a...Ch. 11 - DNaseI cuts DNA that is not directly associated...Ch. 11 - 10.17 Histone protein isolated from pea plants...Ch. 11 - 25. The molecular probes used in FISH can detect...Ch. 11 - Experimental evidence demonstrates that the...Ch. 11 - Prob. 27PCh. 11 - Genomic DNA from the nematode worm...Ch. 11 - What function do histone proteins perform in...Ch. 11 - Based on discussions of specific proteins and...
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- 7. Consider the following plasmid (size 6700 bp), with restriction sites at the positions indicated: BamHI + 1 6700 bp 2800 3500 EcoRI BamHI Probearrow_forwardCre-Lox system is used for site-specific modification of DNA for genetic engineering applications. The reporter gene construct shown below is used to test the Cre-Lox system. Cells are transfected with the construct shown below and the activity of the constructs is determined by visualizing the cells with a fluorescence microscope. Match the following conditions with the expected cell observations. Hint: Make sure you note the position of the Start and Stop. The GFP and RFP genes shown do not have start codons. Cre/Lox Reporter Gene ATG LoxP CMV-Pro A. No Expression GFP Stop B. GFP Only LoxP Absence of Cre Cells treated with drug that induces expression of Cre RFP C. RFP Only A. Image A B. Image B C. Image C D Image D express ? D. RFP and GFParrow_forwardBacterial systems serve as an excellent model to express proteins but has a disadvantage – what is that disadvantage? List the points to differentiate three classes of restriction enzymes? Give an example of restriction enzyme that has ability to generate blunt and cohesive ends after digestion of DNA?arrow_forward
- What is a restriction enzyme? What structure does it recognize?What type of chemical bond does it cleave? Be as specific aspossible.arrow_forwardExplain:a. functionalization of a sensor array with DNA oligonucleotide capture probes for ribosomalRNA (rRNA) species-specific sequences.b. sandwich hybridization of target rRNA with the capture probe and a horseradish peroxidase-linked DNA oligonucleotide detector probe.arrow_forwardWhy does the probe bind to a specific site on a chromosome?arrow_forward
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