BIOLOGY:THE ESSENTIALS (LL) W/CONNECT
3rd Edition
ISBN: 9781260670929
Author: Hoefnagels
Publisher: MCG CUSTOM
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Textbook Question
Chapter 11, Problem 3PIT
Add the terms restriction enzyme, plasmid, virus, DNA polymerase and short tandem repeat to this concept map.
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Make a chart (with diagrammatic representation) showing a restriction enzyme, the substrate DNA on which it acts, the site at which it cuts DNA and the product it produces
Draw a diagram of DNA replication that has the double-stranded DNA with the replication bubble, has all 6 enzymes, two leading strands including their primers, and demonstrates how the lagging strand is replicated.
create a concept map illustrating the relationships among these key terms: 1. Foreign DNA; 2. Restriction endonuclease; 3. Ligase; 4. Plasmid; 5. Origin of replication; 6. Recombinant Plasmid;7. Multiple cloning sites.
Chapter 11 Solutions
BIOLOGY:THE ESSENTIALS (LL) W/CONNECT
Ch. 11.1 - What is DNA technology?Ch. 11.1 - In what fields is DNA technology useful?Ch. 11.2 - What are some uses for transgenic organisms?Ch. 11.2 - What are the steps in producing a transgenic...Ch. 11.2 - How do tagged nucleotides participate in first...Ch. 11.2 - What is the function of 98.5 of the human genome...Ch. 11.2 - How does PCR work, and why is it useful?Ch. 11.2 - How are short tandem repeats used in DNA...Ch. 11.2 - Why do investigators sometimes analyze...Ch. 11.3 - Describe the differences among embryonic, adult,...
Ch. 11.3 - What are the potential medical benefits of stem...Ch. 11.3 - Summarize the steps scientists use to clone an...Ch. 11.3 - Why is the technique used to clone mammals called...Ch. 11.4 - Explain how and why a researcher might use a DNA...Ch. 11.4 - Compare and contrast preimplantation genetic...Ch. 11.4 - Prob. 3MCCh. 11.4 - Describe how CRISPR-Cas9 targets a specific gene...Ch. 11.4 - Prob. 5MCCh. 11 - If a restriction enzyme cuts between G and A...Ch. 11 - Which of the following is not a reason that...Ch. 11 - The function of electrophoresis is to a. break a...Ch. 11 - Why is PCR useful? a. Because it replicates all...Ch. 11 - Suppose an investigator at the scene of a murder...Ch. 11 - What is an induced pluripotent stem cell? a. A...Ch. 11 - Dolly the sheep was the first clone of an adult...Ch. 11 - Prob. 8MCQCh. 11 - Preimplantation genetic diagnosis would be least...Ch. 11 - What is the role of a virus in gene therapy? a. It...Ch. 11 - What techniques might researchers use to produce...Ch. 11 - Transgenic crops often require fewer herbicides...Ch. 11 - Describe why sorting DNA fragments by size is...Ch. 11 - Explain how the ingredients in a PCR reaction tube...Ch. 11 - Prob. 5WIOCh. 11 - Why are entire genomes not used for DNA profiling?Ch. 11 - Prob. 7WIOCh. 11 - Mature neurons in the brain do not replicate. Why...Ch. 11 - Unneeded genes in an adult animal cell are...Ch. 11 - Scientists are interested in cloning an extinct...Ch. 11 - Prob. 11WIOCh. 11 - Prob. 12WIOCh. 11 - Use the Internet to research an application of...Ch. 11 - Prob. 14WIOCh. 11 - Review Burning Question 11.11, which describes the...Ch. 11 - Review the Survey the Landscape figure in the...Ch. 11 - How does PCR related to DNA profiling and...Ch. 11 - Add the terms restriction enzyme, plasmid, virus,...Ch. 11 - How is a patient who receives gene therapy similar...
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- involved in the transfer of multiple drug resistance from one cell to another? * a.Transposition b. Conjugation with a cell with chromosomal drug resistance appears in the genome of a bacteriophage that has infected it. c. Transformation of chromosomal genes d. Conjugation with a cell with a free plasmid carrying drug resistancearrow_forwardMatch. This is a diagram showing..? The structure identified by A The structure identified by B 1. plasmid 2. normal bacterial cell division 3. mRNA 4. horizontal gene transfer 5. Bacterial DNA in a bacteriophage 6. a virus such as SARS-CoV-2arrow_forwardChoose the combination of answers that most accurately completes the statement. Which gene is incorporated into plasmids to detect recombinant cells? a. restriction endonuclease c. a gene for antibiotic resistance b. virus receptors d. reverse transcriptasearrow_forward
- Compare the process of polymerase chain reaction (PCR) to DNA replication. Discuss three modifications of the PCR process and which enzymes/molecules these modification replace in the usual DNA replication processarrow_forwardMatch term to definition Anneal Replication Translation Transcription a. Process of making a copy of DNA molecule B. Process of synthesizing proteins from an RNA template C. To allow base pairing between complementary single polynucleotide strands so double stranded segments form. D. Process of synthesizing rna using a dna templatearrow_forwardChoose the combination of answers that most accurately completes the statement.The function of ligase is to a. rejoin segments of DNA c. synthesize cDNA b. make longitudinal cuts in DNA d. break down ligamentsarrow_forward
- Use the gel to answer the following questions. You will be constructing a map of the plasmid, pDiddy. If the Nco1/EcoRI double digests produces 3 fragments what are their sizes? 2.5 kb, 250 b and 250 b 3.0 kb 2.0 kb, 500 b and 500 b 3.0 kb, 1.0kb and 1.0 kbarrow_forwardWhich one is an example of nucleic acid hybridization? A the template and newly synthesized DNA strands in replication B reverse transcription of a human RNA into cDNA C association of two strands, one synthetic, by complementarity D synthesis of copy strands by PCR E gene cloning in bacteriaarrow_forwardWhy must a genetically engineered plasmid contain a genetic marker? a.) to prevent the construction of an artificial chromosome b.) to separate cells that contain recombinant DNA from those that do not c.) to produce multiple copies of the recombined plasmid after heat treatment d.) to break apart the circular plasmid and introduce another DNA fragmentarrow_forward
- Use the gel to answer the following questions. You will be constructing a map of the plasmid, pDiddy. IF the EcoRI/BamHI double digest produces 3 fragments with only two sizes, what are their sizes? 2.5 kb, 250 b and 250 b 3.0 kb 2.0 kb 500 b and 500 b 3.0 kb 1.0kb and 1.0 kbarrow_forwardSecond part asks: Explain the roles two of those enzymes would play in obtaining the recombinant plasmidsarrow_forwardThe function of a restriction enzyme is to a. prevent the movement of DNA outside the nucleus b. separate the DNA double helix c. cut the nucleotide sequence at a specific location in DNA d. proofread DNA for accidental damages and corrects these errorsarrow_forward
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