Genetic Analysis: An Integrated Approach (3rd Edition)
3rd Edition
ISBN: 9780134605173
Author: Mark F. Sanders, John L. Bowman
Publisher: PEARSON
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Chapter 11, Problem 5P
UV irradiation causes damage to bacterial DNA. What kind of damage is frequently caused and how does photolyase repair the damage?
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DNA damage in the form of dimers induced by UV lightcan be repaired by many bacteria using ______ ______ or pho-toreactivation, which occurs when the bacteria get back intothe presence of visible light, or by dark repair, which can occurin the presence or absence of visible light and requires several______ -controlled reactions.
Chapter 11 Solutions
Genetic Analysis: An Integrated Approach (3rd Edition)
Ch. 11 - 11.1 Identify two general ways chemical mutagens...Ch. 11 - 11.2 Nitrous acid and (BU) alter DNA by different...Ch. 11 - 11.3 What is the difference between a transition...Ch. 11 - What is the difference between a synonymous...Ch. 11 - 11.5 UV irradiation causes damage to bacterial...Ch. 11 - Ultraviolet (UV) radiation is mutagenic.
What...Ch. 11 - Researchers interested in studying mutation and...Ch. 11 - The effect of base - pair substitution mutations...Ch. 11 - Describe the purpose of the Ames test. How are...Ch. 11 - 11.10 In numerous population studies of...
Ch. 11 - 11.11 Two different mutations are identified in a...Ch. 11 - What is the phenotype effect of inserting a Ds...Ch. 11 - 11.13 Answer the following questions concerning...Ch. 11 - Several types of mutation are identified and...Ch. 11 - 11.15 A sample of the bacterium is exposed to...Ch. 11 - 11.16 A strain of is identified as having a null...Ch. 11 - Describe the difference between DNA transposons...Ch. 11 - 11.18 How are flanking direct repeat sequences...Ch. 11 - 11.19 Using the adeninethymine base pair in this...Ch. 11 - The partial amino acid sequence of a wild-type...Ch. 11 - Prob. 21PCh. 11 - 11.22 Many human genes are known to have homologs...Ch. 11 - The fluctuation test performed by Luria and...Ch. 11 - In this chapter, three features of genes or of DNA...Ch. 11 - Briefly compare the production of DNA double -...Ch. 11 - During mismatch repair, why is it necessary to...Ch. 11 - 11.27 Following the spill of a mixture of...Ch. 11 - 11.28 In an Ames test using Salmonella bacteria a...Ch. 11 - A wild - type culture of haploid yeast is exposed...Ch. 11 - A fragment of a wild - type polypeptide is...Ch. 11 - Prob. 31PCh. 11 - Alkaptonuria is a human autosomal recessive...Ch. 11 - 11.33 In an experiment employing the methods of...Ch. 11 - Using your knowledge of DNA repair pathways choose...Ch. 11 - 11.35 Ataxia telangiectasia is a human inherited...Ch. 11 - A geneticist searching for mutations uses the...Ch. 11 - 11.37 In a mousebreeding experiment a new mutation...Ch. 11 - 11.38 Considering the Dumbo mutation in a Problem,...Ch. 11 - 11.39 Thinking back to the discussion of...Ch. 11 - 11.40 Common baker’s yeast () is normally grown at...Ch. 11 - 11.41 The two gels illustrated below contain...
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- Why is UV-C is more likely to harm DNA than UV-A?arrow_forwardContrast the various types of DNA repair mechanisms known to counteract the effects of UV radiation. What is the role of visible light in repairing UV-induced mutations?arrow_forwardIn bacterial transforamtion, how should P+ and P- tubes look like after a successful transformation?arrow_forward
- What properties in microorganisms were researchers at SINTEF and NTNU looking for? What made them select Micrococcus luteus as a candidate organism? Describe the steps involved in genetic engineering, prior to commercial production of the potential `UVAblue’ sunscreen. What damage do long wave UV radiations induce in human cells?arrow_forwardExplain why base excision repair, nucleotide excision repair, and mismatch repair—which all require nucleases to excise damaged DNA—require DNA ligase.arrow_forwardIdentify the role of ubiquitin and describe the enzymes required for ubiquitinationarrow_forward
- In preparation for transformation, why do you think we used calcium chloride and magnesium chloride in making the cells competent? Would your results be different if monovalent ions were used instead of divalent ions? Why do you think so?arrow_forwardIs natural bacterial transformation efficient?arrow_forwardCompare and contrast nucleotide excision repair and base excision repair. Diagram both very generally. Descriptions should have at least two sentences.arrow_forward
- When DNA probes are used to detect specific sequence similarities in bacterial DNA, the probe is heated and the two strands of DNA are separated. Why must the probe DNA be heated?arrow_forwardWhat is the significance of using thermostable DNA polymerase for Polymerase Chain Reaction?arrow_forwardExplain how you would seperate Taq polymerase(PDB: 1TAQ) using one or series of chromatography methods from a cell extract.arrow_forward
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