Concept explainers
Imagine you have found a small quantity of DNA. Fill in the following diagram, which outlines a series of DNA technology experiments you could perform to study this DNA.
To complete: The given map showing the steps involved in recombinant DNA (rDNA) technology.
Introduction: Recombinant DNA technology is a technique used to produce novel genetic combinations by combining the genes from two different organisms and introduces them inside a host in order to proliferate and grow.
Answer to Problem 1CC
Pictorial representation: Fig. 1 shows the completed map of the steps involved in recombinant DNA technology.
Fig. 1 The steps involved in recombinant DNA (rDNA) technology.
Explanation of Solution
(a)
Correct answer: PCR
Explanation: The polymerase chain reaction (PCR) is used to get multiple copies of DNA within a short period. In PCR, for synthesizing multiple copies of a specific DNA fragment, DNA nucleotide primers are used. Hence, the correct answer is PCR.
(b)
Correct answer: A restriction enzyme
Explanation: Restriction enzymes are a group of enzymes used to cut the DNA by binding to certain specific DNA sequence sites called as restriction sites. It forms single-stranded ends called as sticky ends. The sticky ends help the DNA to bind with the other DNA fragment that has the same single-stranded ends by complementary base pairing. Hence, the correct answer is a restriction enzyme.
(c)
Correct answer: Gel electrophoresis
Explanation: Electrophoresis is used to separate the molecules based on their size and charge in the presence of an electric field. Hence, the correct answer is Gel electrophoresis.
(d)
Correct answer: Nucleic acid probe
Explanation: Nucleic acid probe is used for the analysis of the probe, which can specifically bind to the complementary DNA strand. Hence, the correct answer is nucleic acid probe.
(e)
Correct answer: Cloning
Explanation: The process of production of identical copies of the parent cell is called as cloning. Cloning vectors are used in the rDNA technology for the propagation of the desired gene. Hence, the correct answer is cloning.
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Chapter 12 Solutions
Mastering Biology with Pearson eText -- Standalone Access Card -- for Campbell Biology: Concepts & Connections (8th Edition)
- Which of the following best describes the process of DNA sequencing? a. DNA is separated on a gel, and the different bands are labeled with fluorescent nucleotides and scanned with a laser. b. A laser is used to fluorescently label the nucleotides present within the DNA, the DNA is run on a gel, and then the DNA is broken into fragments. c. Nucleotides are scanned with a laser and incorporated into the DNA that has been separated on a gel, and then the DNA is amplified with PCR. d. Fragments of DNA are produced in a reaction that labels them with any of four different fluorescent dyes, and the fragments then are run on a gel and scanned with a laser. e. DNA is broken down into its constituent nucleotides, and the nucleotides are then run on a gel and purified with a laser.arrow_forwardn gel electrophoresis of DNA, the different bands in the final gel form because the DNA molecules _______. a. are from different organisms b. have different lengths c. have different nucleotide compositions d. have different genesarrow_forwardCloning Genes Is a Multistep Process Which enzyme is responsible for covalently linking DNA strands together? a. DNA polymerase b. DNA ligase c. EcoRl d. restriction enzymes e. RNA polymerasearrow_forward
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