Microbiology: An Introduction
12th Edition
ISBN: 9780321929150
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: PEARSON
expand_more
expand_more
format_list_bulleted
Concept explainers
Videos
Textbook Question
Chapter 12, Problem 2R
A mixed culture of Escherichia coli and Penicillium chrysogenum is inoculated onto the following culture media. On which medium would you expect each to grow? Why?
- a. 0.5% peptone in tap water
- b. 10% glucose in tap water
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
Why is the top part of a broth or slant culture heated in a flame immediately after removing the cap?
a.
to kill all the bacteria on the glass
b.
to sterilize the glass in case you touch it with your loop
c.
to stimulate the growth of bacteria in the tube
d.
to create warm air currents that keep other bacteria out
e.
to expand the glass of the test tube top so that the cap will fit more securely
You spread 0.1 mL volume of a 10^(-6) dilution onto a nutrient agar plate. After 24 hours of incubation at 37°C, there were 280 colonies of bacteria on the plate. A.) What is the original concentration (OCD) of bacteria in the stock sample this dilution came from? B.) Using the OCD value from part A, determine the number of colonies that would be expected to grow on a plate that is inoculated with 0.1 mL volume of 10^(-8) dilution from this same stock of bacteria. Show your work for both.
a) explain in your own words how to do a nitrate reduction test to identify unknown bacteria
b) explain in your own words how to perform a phenol red broth (prb) test to help identify unknown bacteria
Chapter 12 Solutions
Microbiology: An Introduction
Ch. 12 - Prob. 1RCh. 12 - A mixed culture of Escherichia coli and...Ch. 12 - Prob. 3RCh. 12 - Prob. 4RCh. 12 - Differentiate cellular and plasmodial slime molds....Ch. 12 - Prob. 6RCh. 12 - Prob. 7RCh. 12 - Prob. 8RCh. 12 - Prob. 9RCh. 12 - DRAW IT A generalized life cycle of the liver...
Ch. 12 - How many phyla are represented in the following...Ch. 12 - Prob. 2MCQCh. 12 - Prob. 3MCQCh. 12 - Fleas are the intermediate host for Dioylium...Ch. 12 - Prob. 5MCQCh. 12 - Prob. 6MCQCh. 12 - The definitive host for Plasmodium vivax is a....Ch. 12 - Prob. 8MCQCh. 12 - Prob. 9MCQCh. 12 - Prob. 10MCQCh. 12 - Alexandrium (red tide) has been called a plant...Ch. 12 - The life cycle of the fish tapeworm...Ch. 12 - Trypanosoma brucei gambiense-part (a) in the...Ch. 12 - Prob. 1CAECh. 12 - Prob. 2CAECh. 12 - A teenaged male in California complained of...
Additional Science Textbook Solutions
Find more solutions based on key concepts
Plants use the process of photosynthesis to convert the energy in sunlight to chemical energy in the form of su...
Campbell Essential Biology with Physiology (5th Edition)
CAUTION How can evolutionary fitness be estimated? a. Document how long individuals survive. b. Count the numbe...
Biological Science (6th Edition)
Your bore cells, muscle cells, and skin cells look different because a. different kinds of genes are present in...
Campbell Essential Biology (7th Edition)
5.4 Genes E and H are syntenic in an experimental organism with the genotype . Assume
that during each meiosis,...
Genetic Analysis: An Integrated Approach (3rd Edition)
Why are BSL-4 suits pressurized? Why not just wear tough regular suits?
Microbiology with Diseases by Body System (5th Edition)
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Each Escherichia coli cell weighs 5ng. How many cells are present in a colony that weighs 1mg?arrow_forwardThe following results were obtained from a disk diffusion test for a strain of Escherichia coli. Chemotherapeutic Zone of inhibition (mm) A 7 B 10 C 18 D 3 Which drug above (A, B, C, or D) is most effective against this E. coli strain? ***Which drug above (A, B, C, or D) is most effective against Staphylococcus aureus? Explain your answer.arrow_forwardIn August 2021, there was a multi-state outbreak of melioidosis, an infectious disease caused by the bacterium Burkholderia pseudomallei, thought to arise from imported products. Burkholderia pseudomallei can be grown in culture. In terms of human health, why might it be critical to grow a pure culture of bacteria?arrow_forward
- The bacterium Dontuplodemeus tocheggus has a generation time of 20 minutes. Starting with one cell in 500 mL of culture medium, how many minutes would it take to reach a cell density of approximately one million cells per mL? Assume all cells remain viable. a. 330 b. 660 c. 380 d. 400 e. 580arrow_forwardE.coli was incubated with aeration in a nutrient medium containing two carbon sources provided one at a time, and the following growth curve was made from this culture. a. Explain what happened at the time marked x. b. Which substrate provided “better” growth conditions for the bacteria? How can you tell?arrow_forwardIf you streak a sample from your nose onto a Mannitol salt agar plate and the medium turns yellow, what does that tell you? (choose all that apply) a. The organism is a yeast b. The organism ferments mannitol c. The organism is beta-hemolytic d. The organism is likely Staphylococus aureus e. The organism is acid tolerantarrow_forward
- THIS IS A 2 PART Question a. Is this a good streak? If so, explain why. If not, explain what you would change. b. Is this streak of a pure culture, a mixed culture, or a contaminated culture? Explain your response.arrow_forwardIn a certain culture of bacteria, the rate of increase is proportional to the number present. (a) if it is found that the number doubles in 6 hours, how many may be expected at the end of 18 hours? (b) If there are 10^2 at the end of 4 hours and 8(10^2) at the end of 8 hours, how many were at the beginning?arrow_forwardA bacterial organism is used to inoculate mannitol broth medium. Following incubation the broth is yellow. This indicates that the organism: A) can use lactose and glucose B) cannot use lactose C) can use mannitol D) cannot use mannitolarrow_forward
- A culture of E. coli is diluted as follows:(1) 65mL are added to 435mL of water.(2) 10uL from (1) are then added to 9.99mL of water.(3) A 10-3 dilution is made from tube # (2).(4) 100uL from (3) are plated for a pour plate and incubated. There were 34 colonies counted on one quarter of the plate following incubation. a) What was the overall dilution?b) How many cfu/mL were present in the original culture?c) How many milliliters of water is needed to make a 10-3 dilution using 1000 uL from the original culture?arrow_forwardMacConkey (MAC) Agar is used to isolate gram-negative enteric bacteria.Peptone provides nutrients necessary for growth.Bile salts and crystal violet inhibit the growth of gram positive bacteria.Lactose is a carbohydrate. When metabolized, colonies turn the medium pink.Which organism/s will grow in the medium? a. Escherichia coli b. Pseudomonas aeruginosa c. Staphylococcus aureus d. Escherichia coli & Pseudomonas aeruginosa e. Pseudomonas aeruginosa & Staphylococcus aureusarrow_forwardWhich of the following statements is a reason why Acid-Fast Bacteria resist the Gram stain a) Because their cell wall architecture varies from both gram-negative and gram-positive bacteria. b) Because they lack the peptidoglycan layer. c) Because they have a thick peptidoglycan layer. d) Because they have a thick layer of fatty acids.arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Comprehensive Medical Assisting: Administrative a...NursingISBN:9781305964792Author:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy CorreaPublisher:Cengage Learning
Comprehensive Medical Assisting: Administrative a...
Nursing
ISBN:9781305964792
Author:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa
Publisher:Cengage Learning
Microbial Nutrition and Growth; Author: Scientist Cindy;https://www.youtube.com/watch?v=rK3UkyWjkl8;License: Standard YouTube License, CC-BY