Brock Biology Of Microorganisms - Package
14th Edition
ISBN: 9780134462660
Author: MADIGAN
Publisher: PEARSON
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Chapter 12, Problem 3AQ
Imagine that you have been given several bacterial strains from various countries around the world and that all the strains are thought to cause the same gastrointestinal disease and to be genetically identical. Upon carrying out a DNA fingerprint analysis of the strains, you find that four different strain types are present. What methods could you use to test whether the different strains are actually members of the same species?
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Chapter 12 Solutions
Brock Biology Of Microorganisms - Package
Ch. 12.1 - What characteristics would have made the surface...Ch. 12.1 - How do we know when oceans were first present on...Ch. 12.1 - What lines of reasoning support the hypothesis...Ch. 12.2 - Why is the origin of cyanobacteria considered a...Ch. 12.2 - What caused the development of banded iron...Ch. 12.2 - What lines of evidence indicate that microbial...Ch. 12.3 - What evidence supports the idea that the...Ch. 12.3 - Prob. 2MQCh. 12.3 - In what ways are modern eukaryotes a combination...Ch. 12.4 - Prob. 1MQ
Ch. 12.4 - Prob. 2MQCh. 12.4 - Prob. 3MQCh. 12.5 - How are DNA sequences obtained for phylogenetic...Ch. 12.5 - What does a phylogenetic tree depict?Ch. 12.5 - Prob. 3MQCh. 12.6 - What are the different processes that give rise to...Ch. 12.6 - What is the difference between selection and...Ch. 12.6 - In the experiment of Figure 13.12, why did the...Ch. 12.7 - What is the difference between the core and pan...Ch. 12.7 - What kind of recombination might have the greatest...Ch. 12.7 - What effects do deletions have on the evolution of...Ch. 12.8 - What is the difference between taxonomy and...Ch. 12.8 - What are some key criteria from the phylogenetic...Ch. 12.8 - How many species of Bacteria and Archaea have been...Ch. 12.9 - What class of genes is used in MLST analyses?Ch. 12.9 - How is ribotyping different from rep-PCR?Ch. 12.9 - What is FAME analysis?Ch. 12.10 - What roles do culture collections play in...Ch. 12.10 - What is the IJSEM and what taxonomic function does...Ch. 12.10 - Why might viable cell cultures be of more use in...Ch. 12 - Prob. 1RQCh. 12 - Prob. 2RQCh. 12 - Prob. 3RQCh. 12 - What is the endosymbiotic hypothesis for the...Ch. 12 - Prob. 5RQCh. 12 - REVIEW QUESTIONS
6. What major physiological and...Ch. 12 - Prob. 7RQCh. 12 - Prob. 8RQCh. 12 - Prob. 9RQCh. 12 - What is the difference between a gene tree and an...Ch. 12 - Prob. 11RQCh. 12 - Prob. 12RQCh. 12 - What is fitness? To what degree does fitness...Ch. 12 - What are some processes that influence the content...Ch. 12 - Prob. 15RQCh. 12 - What is the "species problem" and why is the...Ch. 12 - Prob. 17RQCh. 12 - Prob. 18RQCh. 12 - Prob. 19RQCh. 12 - Prob. 20RQCh. 12 - Prob. 21RQCh. 12 - Compare and contrast the physical and chemical...Ch. 12 - For the following sequences, construct the...Ch. 12 - Imagine that you have been given several bacterial...Ch. 12 - Imagine that you have discovered a new form of...
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- If you were a bacterium, what genetic traits would you find most helpful to acquire?arrow_forwardIf the PCR analysis of your sample did not work can you suggest why? How can you test if this is true?arrow_forwardYou have discovered a new species of archaea from a hot spring in Yellowstone National Park. After growing a pure culture of this organism, what strategy might you employ to sequence its genome?arrow_forward
- Using the question in the image below, identify the locations of each mutation in strains 1, 2, and 3arrow_forwardDNA fingerprinting involves using restriction enzymes to cut DNA at a specific sequence, resulting in many fragments of different lengths. Gel electrophoresis then separates the fragments according to size. DNA fingerprints produced from four different individuals is shown below.The DNA for Individuals 3 and 4 could NOT be Select one: a. mitochondrial DNA from two people who have the same maternal grandmother (both their mothers had the same mother) b. mitochondrial DNA from two people who have the same paternal grandmother (both their fathers had the same mother) c. nuclear DNA from identical twins d. nuclear DNA isolated from a hair left at a crime scene and a buccal swab from a suspect who was present at the crimearrow_forwardUsing what you now know about restriction enzymes, why do you suppose that wells in the gel contained more than one band?arrow_forward
- Imagine you wanted to preserve the dwindling populations of giant pandas by developing breeding programs for captive pandas around the world. To preserve the maximum genetic diversity, you want to mate the individuals that are least related to each other. Which of the following methods of analysis would be most helpful in determining which of the captive pandas were related? a. PCR b. STR analysis c. DNA microinjection d. DNA sequencingarrow_forwardThe GC content of Micrococcus is 66 - 75% and of Staphylococcus is 30-40 % moles, from this information would you conclude that these organisms are related? Include an explanation of why GC content is a viable method by which to identify the relatedness of organisms. – In your explanation of “why”, include information of why we are able to use genetic techniques to identify organisms or determine their relatedness, and specifically why GC content can help determine these.arrow_forwardIn another experiment, Griffith mixed heat killed S strain with live R strain bacteria and injected the mixture into mice. A. What strain of the bacteria was found in the blood samples of the mice? B. What were the results of this experiment? C. What conclusion did he reach based on these results?arrow_forward
- If a DNA sample was GC-rich, what would you need to do to adjust your PCR reaction and why?arrow_forwardBASED ON THE PICTURE SHOWN, WHO WILL BE EXCLUDED BASED ON DNA PROFILING? EXPLAIN YOUR ANSWERarrow_forwardWhat are some possible reasons that researchers might be interested in identifying the gene that causes a genetic disease such as ICA? In other words, what benefits might result from this research?arrow_forward
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