Mutation is defined as the permanent alteration in the nucleotide sequence of DNA. Due to a mutation in the nucleotide sequence of DNA, it codes for different protein. It is caused by environmental factors such as UV radiations, ionizing and non-ionizing radiation several chemicals, such as ethidium bromide.

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Chapter 12, Problem 3MC

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Mutation is defined as the permanent alteration in the nucleotide sequence of DNA. Due to a mutation in the nucleotide sequence of DNA, it codes for different protein. It is caused by environmental factors such as UV radiations, ionizing and non-ionizing radiation several chemicals, such as ethidium bromide.

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a. This piece of DNA is cut by EcoRI, the resulting fragments are separated by gel electrophoresis, and the gel is stained with ethidium bromide. Draw a picture of the bands that will appear on the gel. b. If a mutation that alters EcoRI site 1 occurs in this piece of DNA, how will the banding pattern on the gel differ from the one that you drew in part a? c. If mutations that alter EcoRI sites 1 and 2 occur in this piece of DNA, how will the banding pattern on the gel differ from the one that you drew in part a? d. If 1000 bp of DNA were inserted between the two restriction sites, how would the banding pattern on the gel differ from the one that you drew in part a? e. If 500 bp of DNA between the two restriction sites were deleted, how would the banding pattern on the gel differ from the one that you drew in part a?

1. A.) Deletion mutation is a loss of a single base by damage. B.) Point mutation is when a nucleotide is paired with a wrong nucleotide during RNA replication. a. Statement A is correct b. Statement B is correct c. Both A and B are correct d. Both A and B are incorrect Answer: 2. This is a process that produces a change in the structure of the DNA by forming adenine rather than guanine. a. Reverse transcription of guanine b. Alkylation of the 06-Guanine c. Reverse translation of guanine d. Methylation of guanine bases Answer: 3. This type of DNA repair happens when you are removing a single damaged base of DNA strand. a. Nucleotide repair b. Base excision repair c. Strand break repair d. Base nucleotide replacement Answer: