Genetic Analysis: An Integrated Approach (3rd Edition)
3rd Edition
ISBN: 9780134605173
Author: Mark F. Sanders, John L. Bowman
Publisher: PEARSON
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Chapter 12, Problem 8P
The CAP binding site in the lac promoter is the location of positive regulation of gene expression for the operon. Identify what binds at this site to produce positive regulation, under what circumstances binding occurs, and how binding exerts a positive effect.
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A mutation prevents the catabolite activator protein (CAP) from binding to the promoter in the lac operon. What will the effect of this mutation be on the transcription of the operon?
Under what conditions would you expect to see high levels of expression of the structural genes in the lac operon?
Group of answer choices
Lactose is absent and glucose is absent
Lactose is absent and glucose is present
Lactose is present and glucose is present
None of these
Lactose is present and glucose is absent
The TRP operon functions by attenuation. Briefly explain how attenuation works and why it is necessary, in addition to the repressor protein, in regulating the operon.
Chapter 12 Solutions
Genetic Analysis: An Integrated Approach (3rd Edition)
Ch. 12 - 12.1 Bacterial genomes frequently contain groups...Ch. 12 - Transcriptional regulation of operon gene...Ch. 12 - Why is it essential that bacterial cells be able...Ch. 12 - Identify similarities and differences between an...Ch. 12 - The transcription of -galactosidase and permease...Ch. 12 - 12.6 Is attenuation the product of an allosteric...Ch. 12 - The trpL region contains four repeated DNA...Ch. 12 - The CAP binding site in the lac promoter is the...Ch. 12 - What role does cAMP play in transcription of lac...Ch. 12 - How would a cap- mutation that produces an...
Ch. 12 - Explain the circumstances under which attenuation...Ch. 12 - Consider the transcription of genes of the...Ch. 12 - Describe the lytic and lysogenic life cycles of ...Ch. 12 - 12.14 Define antisense RNA, and describe how it...Ch. 12 - 12.15 Attenuation of trp operon transcription is...Ch. 12 - 12.16 In the lac operon, what are the likely...Ch. 12 - Identify which of the following lac operon haploid...Ch. 12 - Prob. 18PCh. 12 - 12.19 List possible genotypes for lac operon...Ch. 12 - Suppose each of the genotypes you listed in parts...Ch. 12 - 12.21 Four independent mutants (mutants A to D)...Ch. 12 - Suppose the lac operon partial diploid...Ch. 12 - What is a riboswitch? Describe the riboswitch...Ch. 12 - 12.24 A repressible operon system, like the trp...Ch. 12 - 12.25 What is the likely effect of each of the...Ch. 12 - 12.26 Suppose that base substitution mutations...Ch. 12 - 12.27 Two different mutations affect. Mutant...Ch. 12 - How would mutations that inactivate each of the...Ch. 12 - The bacterial insertion sequence IS 10 uses...Ch. 12 - For an E. coli strain with the lac operongenotype...Ch. 12 - 12.31 How could antisense RNA be used as an...Ch. 12 - 12.32 Section describes the function of tRNA...Ch. 12 - The following hypothetical genotypes have genes A,...Ch. 12 - 12.34 Northern blot analysis is performed on...Ch. 12 - Prob. 35PCh. 12 - Prob. 36PCh. 12 - 12.37 The electrophoresis gel shown in part (a) is...
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- Explain why inactivation of the O1 sequence of the lac operator almost completely abolishes repression of the lac operon.arrow_forwardTrue or false: The lac operon is regulated by the binding of an enhancer to sequences just upstream of the promoterarrow_forwardIf glucose levels in the cell are high and lactose is available from the environment, what is the status of transcription of the lac operon genes? Explain your answer from both an evolutionary perspective and in terms of negative and positive regulation of the operon?arrow_forward
- When referring to attenuation in the regulation of the trp operon it would be safe to say that, when there are high levels of tryptophan available to the organism, ________. tryptophan inactivates the repressor protein translation termination of the trp operon is likely the trp operon is transcribed at relatively high levels the ribosomes stall during translation of the attenuator region transcription termination at the attenuator region is likelyarrow_forwardIf glucose is not available, but lactose is available from the environment, what is the status of transcription of the lac operon genes? Explain your answer from both an evolutionary perspective and in terms of negative and positive regulation of the operon?arrow_forward"The highest level of transcription of the E. coli lac operon occurs when CAP (+cAMP) is bound to the CAP site and LacI is not bound to the operator." The preceding statement is... True or False?arrow_forward
- Describe the probable genetic defect that abolishes the sensitivity of the lac operon to the absence of glucose when other metabolic operons continue to be sensitive to the absence of glucose.arrow_forwardDescribe how the components of the trp operon system are affected by a mutation in trpC? Why is it that once enviornmental tryptophan is inserted into the cell, only the trp repressor is activated (turned on)?arrow_forward. Mutants were isolated in which the constitutive phenotype of a missense lacI mutation was suppressed.That is, the operon was now inducible. These suppressor mutations mapped to the operon, not to the lacIgene. What could these mutations be?arrow_forward
- Why it is essential to the positive stimulation of transcription of the lac operon ?arrow_forwardFor the following types of transcriptional control, indicate whether the protein produced by the regulator gene will be synthesized initially as an active repressor or as an inactive repressor. Q. Negative control in an inducible operonarrow_forwardIf CAP is bound to the Promoter of the Lac Operon and the repressor protein is not bound to the operator, which of the following is true: a.Glucose Levels are low & Lactose is absent b.Glucose is absent & Lactose is present c.cAMP is low & Lactose is Absent d.Glucose and Lactose are Present e.cAMP is low & Lactose is presentarrow_forward
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