Campbell Essential Biology with Physiology (6th Edition)
6th Edition
ISBN: 9780134711751
Author: Eric J. Simon, Jean L. Dickey, Jane B. Reece
Publisher: PEARSON
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Chapter 12, Problem 9SQ
Summary Introduction
To determine: The correct order of steps of human gene therapy.
Introduction: Human gene therapy is used for the treatment of diseases but inserting new or altered gene in affected person. In some cases where the disease is caused by the presence of mutant allele, the therapy is done by replacing the mutant allele with normal allele.
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Which of the following is a concern of in-vivo gene therapy?
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The NIH Recombinant DNA Advisory Committee has what responsibility? a) Approving all forms of human clinical gene therapy b) Identifying all major risks to the human subjects in a specific research protocol c )Reviewing clinical trials involving human gene transfer d) Analyzing genomes and determining whether they appear mutagenic
Put the following tasks in the order they would occur during a DNA cloning experiment. a. using DNA ligase to seal DNA fragments into vectors b. using a probe to identify a clone in the library c. sequencing the DNA of the clone d. making a DNA library of clones e. cutting genomic DNA with restriction enzymes
Chapter 12 Solutions
Campbell Essential Biology with Physiology (6th Edition)
Ch. 12 - Suppose you wish to create a large batch of the...Ch. 12 - A carrier that moves DNA from one cell to another,...Ch. 12 - In making recombinant DNA, what is the benefit of...Ch. 12 - A paleontologist has recovered a bit of organic...Ch. 12 - Why do DNA fragments containing STR sites from...Ch. 12 - Prob. 6SQCh. 12 - Prob. 7SQCh. 12 - Name the steps of the whole-genome shotgun method.Ch. 12 - Prob. 9SQCh. 12 - Prob. 10IMT
Ch. 12 - Prob. 11IMTCh. 12 - Prob. 12IMTCh. 12 - Some scientists once joked that when the DNA...Ch. 12 - Prob. 14PSCh. 12 - Listed below are 4 of the 13 genome sites used to...Ch. 12 - In the not-too-distant future, gene therapy may be...Ch. 12 - Today, it is fairly easy to make transgenic plants...Ch. 12 - Prob. 18BSCh. 12 - Prob. 19BS
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Put the following tasks in the order they would occur during a DNA cloning experiment. a. using DNA ligase to seal DNA fragments into vectors b. using a probe to identify a clone in the library c. sequencing the DNA of the clone d. making a DNA library of clones e. cutting genomic DNA with restriction enzymesarrow_forwardA _______ is a molecule that can be used to carry a fragment of DNA into a host organism. a. cloning vector c. GMO b. chromosome d. cDNAarrow_forwardCloning Genes Is a Multistep Process In cloning human DNA, why is it necessary to insert the DNA into a vector such as a bacterial plasmid?arrow_forward
- You have extracted a long piece of DNA from a human cell and you want to extract the gene of interest for you to clone it. Assuming that you know the sequence of the DNA what methods can youuse to amplify the gene of interest? A. antibody purification B. polymerase chain reaction C. none of the above D. ligationarrow_forwardChoose the phrase that best describes the role of a cloning vector. A) Separates fragments of DNA B) Detects the presence of specific DNA sample C) Isolates DNA from a donor organism D) Screens for recombinants that have been successfully transformed E) Introduces DNA into a cloning hostarrow_forwardGene therapy can be used to introduce foreign DNA into cells ________. a. for molecular cloning b. by PCR c. of tissues to cure inheritable disease d. all of the abovearrow_forward
- Following are four processes common to most cloning experiments: a) transforming bacteria b) plating bacteria on selective medium c) cutting DNA with restriction endonucleases d) ligating DNA fragments Place components of this list in the order in which they would most likely occur during a cloning experiment.arrow_forwardWhich of the following would be considered a transgenic organism? A. a human whose human blood-clotting gene was silenced B. a fern grown in cell culture from a single fern root cell C. a rat with rabbit hemoglobin genesarrow_forwardCRISPR techniques allow scientists to modify specific genes while sparing all others, thus clarifying the association between a given gene and its consequence to the organism. How can this help to create better antibiotics or to make white blood cells resistant to HIV infection? Give your examples. What is the future of CRISPR?arrow_forward
- Make a list of ethical considerations for using gene therapy.arrow_forwardExplain how you, would correct a genetic defect if you have to make use of a retrovirus. Give an in-depth discussion of the purpose of each experimental step.arrow_forwardWrite the advantages and disadvantages of applying such application in the DNA of an organism. a. Drug delivery systems that are based on bacterial or viral host. b. Producing genetically modified organisms to enhance food production. c. Combining two or more genes in one organism to enhance its traits. d. DNA manipulation of certain species to produce organs for harvesting.arrow_forward
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