Brock Biology of Microorganisms (15th Edition)
15th Edition
ISBN: 9780134261928
Author: Michael T. Madigan, Kelly S. Bender, Daniel H. Buckley, W. Matthew Sattley, David A. Stahl
Publisher: PEARSON
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Chapter 12.2, Problem 1CR
Summary Introduction
Various types of vectors are used for cloning purposes such as plasmids, viruses, artificial chromosomes, and cosmids. The use of these cloning vectors is used mainly depending on the length of the DNA sequence to be cloned and the host. The vector containing gene of interest is inserted into the selected host for replication.
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Chapter 12 Solutions
Brock Biology of Microorganisms (15th Edition)
Ch. 12.1 - Why is a primer needed at each end of the DNA...Ch. 12.1 - How does RT-PCR differ from traditional PCR?Ch. 12.1 - Prob. 3MQCh. 12.1 - Describe the basic principles of gene...Ch. 12.2 - What is the purpose of molecular cloning?Ch. 12.2 - Prob. 2MQCh. 12.2 - Prob. 3MQCh. 12.2 - Prob. 1CRCh. 12.3 - How can the bacteriophage T7 promoter be used to...Ch. 12.3 - What major advantage does cloning mammalian genes...
Ch. 12.3 - Prob. 3MQCh. 12.3 - Prob. 1CRCh. 12.4 - How can site-directed mutagenesis be useful to...Ch. 12.4 - What is used to alter more than a few base pairs...Ch. 12.4 - What are knockout mutations?Ch. 12.4 - What does site-directed mutagenesis allow you to...Ch. 12.5 - What is a reporter gene? The product of which...Ch. 12.5 - Prob. 2MQCh. 12.5 - Describe two widely used reporter genes.Ch. 12.6 - Prob. 1MQCh. 12.6 - Prob. 2MQCh. 12.6 - Prob. 3MQCh. 12.6 - Prob. 1CRCh. 12.7 - Prob. 1MQCh. 12.7 - Give an example of a genetically modified plant...Ch. 12.7 - How have transgenic salmon been engineered to...Ch. 12.7 - What is the Ti plasmid and how has it been of use...Ch. 12.8 - Explain why recombinant vaccines might be safer...Ch. 12.8 - Prob. 2MQCh. 12.8 - Prob. 3MQCh. 12.8 - What is a subunit vaccine and why are subunit...Ch. 12.9 - Explain why metagenomic cloning gives large...Ch. 12.9 - What types of environments are often sampled to...Ch. 12.9 - Prob. 3MQCh. 12.9 - How has metagenomics been used to find novel...Ch. 12.10 - How has Caldicellulosiruptor been modified to...Ch. 12.10 - Prob. 2MQCh. 12.10 - What has been the limiting factor in engineering...Ch. 12.10 - Prob. 1CRCh. 12.11 - What are biobricks?Ch. 12.11 - Prob. 2MQCh. 12.11 - How was Escherichia coli modified to produce a...Ch. 12.11 - Prob. 1CRCh. 12.12 - Prob. 1MQCh. 12.12 - Prob. 2MQCh. 12.12 - How is recombinant DNA inserted into a genome...Ch. 12.12 - How has the CRISPR editing technology been applied...Ch. 12.13 - Prob. 1MQCh. 12.13 - How can a tRNA be engineered to encode for a...Ch. 12.13 - Prob. 3MQCh. 12.13 - What are some mechanisms for controlling a...Ch. 12 - Suppose you have just determined the DNA base...Ch. 12 - Prob. 2AQCh. 12 - Prob. 3AQCh. 12 - Describe how you could recode Escherichia coli to...
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- What is SDS and what part does it play in the alkaline lysis method of preparing plasmid DNA from a culture of bacteria?arrow_forwardHow does restriction enzyme digestion allow for the determination of the correct plasmid construct without having the need for DNA sequencing?arrow_forwardwhich is the delivery techniques can bbe used to best transfer recombinant plasmids into E.coli cells?arrow_forward
- How is Ti plasmid of Agrobacterium tumefaciens modified to convert it into a cloning vector?arrow_forwardWhy are plasmids used as vector for DNA Recombination? What other vectors can be used?arrow_forwardWhy are nucleic acid probes necessary to lyse cells containing plasmids in order to detect the product of the cloned gene?arrow_forward
- Why must the restriction enzyme used for the digestion of TOL plasmid be inactivated immediately after the restriction experiment? How could this be achieved?arrow_forwardWhat is the Ti plasmid and how is it modified for the genetic modification of plants?arrow_forwardWhat is the role of ‘Ori’ in any plasmid?arrow_forward
- What is an engineered plasmid used for? What are the important features of\ engineered plasmids?arrow_forwardWhat does the F plasmid code for?arrow_forwardWhat are some potential difficulties in using plasmid vectors and bacterial host cells to produce large quantitiesof proteins from cloned eukaryotic genes?arrow_forward
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