To produce a protein containing synthetic amino acid, synthetic biologists replaced all TAG stop codons (UAG on the RNA) linked to the open reading frames with TAA stop codons on the chromosome of the Escherichia coli . In addition, synthetic biologists removed the gene encoding releasing factor 1 that involved in the translation termination when the ribosome encounters the UAG on the mRNA. This alternation placed an alternative stop codon at the end of genes, which had TAG codons. The correct length of proteins is still synthesized in the translation process (protein synthesis) and the recoded cell grows normally. In addition, this genetic alteration freed up the UAG stop codon to be reassigned another translational process.

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Chapter 12.13, Problem 3MQ

Summary Introduction

To produce a protein containing synthetic amino acid, synthetic biologists replaced all TAG stop codons (UAG on the RNA) linked to the open reading frames with TAA stop codons on the chromosome of the Escherichia coli. In addition, synthetic biologists removed the gene encoding releasing factor 1 that involved in the translation termination when the ribosome encounters the UAG on the mRNA. This alternation placed an alternative stop codon at the end of genes, which had TAG codons. The correct length of proteins is still synthesized in the translation process (protein synthesis) and the recoded cell grows normally. In addition, this genetic alteration freed up the UAG stop codon to be reassigned another translational process.

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Answer 2

Question

Chapter 12.13, Problem 3MQ

Summary Introduction

To produce a protein containing synthetic amino acid, synthetic biologists replaced all TAG stop codons (UAG on the RNA) linked to the open reading frames with TAA stop codons on the chromosome of the Escherichia coli. In addition, synthetic biologists removed the gene encoding releasing factor 1 that involved in the translation termination when the ribosome encounters the UAG on the mRNA. This alternation placed an alternative stop codon at the end of genes, which had TAG codons. The correct length of proteins is still synthesized in the translation process (protein synthesis) and the recoded cell grows normally. In addition, this genetic alteration freed up the UAG stop codon to be reassigned another translational process.

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Answer 3

Question

Chapter 12.13, Problem 3MQ

Summary Introduction

To produce a protein containing synthetic amino acid, synthetic biologists replaced all TAG stop codons (UAG on the RNA) linked to the open reading frames with TAA stop codons on the chromosome of the Escherichia coli. In addition, synthetic biologists removed the gene encoding releasing factor 1 that involved in the translation termination when the ribosome encounters the UAG on the mRNA. This alternation placed an alternative stop codon at the end of genes, which had TAG codons. The correct length of proteins is still synthesized in the translation process (protein synthesis) and the recoded cell grows normally. In addition, this genetic alteration freed up the UAG stop codon to be reassigned another translational process.

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Answer 4

Question

Chapter 12.13, Problem 3MQ

Summary Introduction

To produce a protein containing synthetic amino acid, synthetic biologists replaced all TAG stop codons (UAG on the RNA) linked to the open reading frames with TAA stop codons on the chromosome of the Escherichia coli. In addition, synthetic biologists removed the gene encoding releasing factor 1 that involved in the translation termination when the ribosome encounters the UAG on the mRNA. This alternation placed an alternative stop codon at the end of genes, which had TAG codons. The correct length of proteins is still synthesized in the translation process (protein synthesis) and the recoded cell grows normally. In addition, this genetic alteration freed up the UAG stop codon to be reassigned another translational process.

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Answer 5

Chapter 12.13, Problem 3MQ

Summary Introduction

To produce a protein containing synthetic amino acid, synthetic biologists replaced all TAG stop codons (UAG on the RNA) linked to the open reading frames with TAA stop codons on the chromosome of the Escherichia coli. In addition, synthetic biologists removed the gene encoding releasing factor 1 that involved in the translation termination when the ribosome encounters the UAG on the mRNA. This alternation placed an alternative stop codon at the end of genes, which had TAG codons. The correct length of proteins is still synthesized in the translation process (protein synthesis) and the recoded cell grows normally. In addition, this genetic alteration freed up the UAG stop codon to be reassigned another translational process.

Answer 6

Chapter 12.13, Problem 3MQ

Summary Introduction

To produce a protein containing synthetic amino acid, synthetic biologists replaced all TAG stop codons (UAG on the RNA) linked to the open reading frames with TAA stop codons on the chromosome of the Escherichia coli. In addition, synthetic biologists removed the gene encoding releasing factor 1 that involved in the translation termination when the ribosome encounters the UAG on the mRNA. This alternation placed an alternative stop codon at the end of genes, which had TAG codons. The correct length of proteins is still synthesized in the translation process (protein synthesis) and the recoded cell grows normally. In addition, this genetic alteration freed up the UAG stop codon to be reassigned another translational process.

Answer 7

Chapter 12.13, Problem 3MQ

Summary Introduction

To produce a protein containing synthetic amino acid, synthetic biologists replaced all TAG stop codons (UAG on the RNA) linked to the open reading frames with TAA stop codons on the chromosome of the Escherichia coli. In addition, synthetic biologists removed the gene encoding releasing factor 1 that involved in the translation termination when the ribosome encounters the UAG on the mRNA. This alternation placed an alternative stop codon at the end of genes, which had TAG codons. The correct length of proteins is still synthesized in the translation process (protein synthesis) and the recoded cell grows normally. In addition, this genetic alteration freed up the UAG stop codon to be reassigned another translational process.

Answer 8

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Answer 11

Ch. 12.1 - Why is a primer needed at each end of the DNA...Ch. 12.1 - How does RT-PCR differ from traditional PCR?Ch. 12.1 - Prob. 3MQ Ch. 12.1 - Describe the basic principles of gene...Ch. 12.2 - What is the purpose of molecular cloning?Ch. 12.2 - Prob. 2MQ Ch. 12.2 - Prob. 3MQ Ch. 12.2 - Prob. 1CR Ch. 12.3 - How can the bacteriophage T7 promoter be used to...Ch. 12.3 - What major advantage does cloning mammalian genes...

Solution Summary: The author explains that synthetic biologists replaced all TAG stop codons (UAG on the RNA) linked to the open reading frames with TAA stops on chromosomes of Escherichia coli.

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