Brock Biology of Microorganisms
15th Edition
ISBN: 9780134626352
Author: MADIGAN
Publisher: PEARSON
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Textbook Question
Chapter 12.3, Problem 1MQ
- How can the bacteriophage T7 promoter be used to control expression of a eukaryotic gene in Escherichia coli?
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How can the bacteriophage T7 promoter be used to controlexpression of a eukaryotic gene in Escherichia coli?
How is the T7 bacteriophage regulated expression exploited in molecular biology?
What part of the E.coli RNA polymerase specifically binds to the -10 and -35 region of an E.coli promoter?
Chapter 12 Solutions
Brock Biology of Microorganisms
Ch. 12.1 - Why is a primer needed at each end of the DNA...Ch. 12.1 - How does RT-PCR differ from traditional PCR?Ch. 12.1 - Prob. 3MQCh. 12.1 - Describe the basic principles of gene...Ch. 12.2 - What is the purpose of molecular cloning?Ch. 12.2 - Prob. 2MQCh. 12.2 - Prob. 3MQCh. 12.2 - Prob. 1CRCh. 12.3 - How can the bacteriophage T7 promoter be used to...Ch. 12.3 - What major advantage does cloning mammalian genes...
Ch. 12.3 - Prob. 3MQCh. 12.3 - Prob. 1CRCh. 12.4 - How can site-directed mutagenesis be useful to...Ch. 12.4 - What is used to alter more than a few base pairs...Ch. 12.4 - What are knockout mutations?Ch. 12.4 - What does site-directed mutagenesis allow you to...Ch. 12.5 - What is a reporter gene? The product of which...Ch. 12.5 - Prob. 2MQCh. 12.5 - Describe two widely used reporter genes.Ch. 12.6 - Prob. 1MQCh. 12.6 - Prob. 2MQCh. 12.6 - Prob. 3MQCh. 12.6 - Prob. 1CRCh. 12.7 - Prob. 1MQCh. 12.7 - Give an example of a genetically modified plant...Ch. 12.7 - How have transgenic salmon been engineered to...Ch. 12.7 - What is the Ti plasmid and how has it been of use...Ch. 12.8 - Explain why recombinant vaccines might be safer...Ch. 12.8 - Prob. 2MQCh. 12.8 - Prob. 3MQCh. 12.8 - What is a subunit vaccine and why are subunit...Ch. 12.9 - Explain why metagenomic cloning gives large...Ch. 12.9 - What types of environments are often sampled to...Ch. 12.9 - Prob. 3MQCh. 12.9 - How has metagenomics been used to find novel...Ch. 12.10 - How has Caldicellulosiruptor been modified to...Ch. 12.10 - Prob. 2MQCh. 12.10 - What has been the limiting factor in engineering...Ch. 12.10 - Prob. 1CRCh. 12.11 - What are biobricks?Ch. 12.11 - Prob. 2MQCh. 12.11 - How was Escherichia coli modified to produce a...Ch. 12.11 - Prob. 1CRCh. 12.12 - Prob. 1MQCh. 12.12 - Prob. 2MQCh. 12.12 - How is recombinant DNA inserted into a genome...Ch. 12.12 - How has the CRISPR editing technology been applied...Ch. 12.13 - Prob. 1MQCh. 12.13 - How can a tRNA be engineered to encode for a...Ch. 12.13 - Prob. 3MQCh. 12.13 - What are some mechanisms for controlling a...Ch. 12 - Suppose you have just determined the DNA base...Ch. 12 - Prob. 2AQCh. 12 - Prob. 3AQCh. 12 - Describe how you could recode Escherichia coli to...
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- What are the two important sequence elements in the prokaryotic gene promoter? How does the RNA polymerase find the promoter?arrow_forwardWhy can it be said that transcription of thebacteriophage T7 genome requires two enzymes?arrow_forwardWhat could be the effect of a base substitution which prevents post-transcriptional removal of an intron?arrow_forward
- Why is an NFR needed at the core promoter for transcription to occur?arrow_forwardWhat is the significance of the fact that human mRNA can be accurately translated in E. coli ?arrow_forwardExplain how elements of the lac operon are exploited to regulate recombinant protein production in E. coli.arrow_forward
- Why are cDNA libraries desirable for the expression of eukaryotic genes in prokaryotes?arrow_forwardHow do we know that eukaryotic transcription factors bind to DNA sequences at or near promoter regions?arrow_forwardWhat sequence elements are found within the core promoter of protein-encoding genes in eukaryotes? Describe their locations and specific functions.arrow_forward
- In the lac operon system, do the inducers act at the transcription or translation level? Explain why it acts in that level in a short but concise way.arrow_forwardWhat role is played by Silencers in transcription of eukaryotic genes ?arrow_forwardWhat is the probability that the palindromic symmetry of the trp repressor target DNA sequence is merely accidental?arrow_forward
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