Mastering Microbiology with Pearson eText -- Standalone Access Card -- for Brock Biology of Microorganisms (15th Edition)
15th Edition
ISBN: 9780134603971
Author: Michael T. Madigan, Kelly S. Bender, Daniel H. Buckley, W. Matthew Sattley, David A. Stahl
Publisher: PEARSON
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Textbook Question
Chapter 12.3, Problem 2MQ
- What major advantage does cloning mammalian genes from mRNA or using synthetic genes have over PCR amplification and cloning of the native gene?
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Chapter 12 Solutions
Mastering Microbiology with Pearson eText -- Standalone Access Card -- for Brock Biology of Microorganisms (15th Edition)
Ch. 12.1 - Why is a primer needed at each end of the DNA...Ch. 12.1 - How does RT-PCR differ from traditional PCR?Ch. 12.1 - Prob. 3MQCh. 12.1 - Describe the basic principles of gene...Ch. 12.2 - What is the purpose of molecular cloning?Ch. 12.2 - Prob. 2MQCh. 12.2 - Prob. 3MQCh. 12.2 - Prob. 1CRCh. 12.3 - How can the bacteriophage T7 promoter be used to...Ch. 12.3 - What major advantage does cloning mammalian genes...
Ch. 12.3 - Prob. 3MQCh. 12.3 - Prob. 1CRCh. 12.4 - How can site-directed mutagenesis be useful to...Ch. 12.4 - What is used to alter more than a few base pairs...Ch. 12.4 - What are knockout mutations?Ch. 12.4 - What does site-directed mutagenesis allow you to...Ch. 12.5 - What is a reporter gene? The product of which...Ch. 12.5 - Prob. 2MQCh. 12.5 - Describe two widely used reporter genes.Ch. 12.6 - Prob. 1MQCh. 12.6 - Prob. 2MQCh. 12.6 - Prob. 3MQCh. 12.6 - Prob. 1CRCh. 12.7 - Prob. 1MQCh. 12.7 - Give an example of a genetically modified plant...Ch. 12.7 - How have transgenic salmon been engineered to...Ch. 12.7 - What is the Ti plasmid and how has it been of use...Ch. 12.8 - Explain why recombinant vaccines might be safer...Ch. 12.8 - Prob. 2MQCh. 12.8 - Prob. 3MQCh. 12.8 - What is a subunit vaccine and why are subunit...Ch. 12.9 - Explain why metagenomic cloning gives large...Ch. 12.9 - What types of environments are often sampled to...Ch. 12.9 - Prob. 3MQCh. 12.9 - How has metagenomics been used to find novel...Ch. 12.10 - How has Caldicellulosiruptor been modified to...Ch. 12.10 - Prob. 2MQCh. 12.10 - What has been the limiting factor in engineering...Ch. 12.10 - Prob. 1CRCh. 12.11 - What are biobricks?Ch. 12.11 - Prob. 2MQCh. 12.11 - How was Escherichia coli modified to produce a...Ch. 12.11 - Prob. 1CRCh. 12.12 - Prob. 1MQCh. 12.12 - Prob. 2MQCh. 12.12 - How is recombinant DNA inserted into a genome...Ch. 12.12 - How has the CRISPR editing technology been applied...Ch. 12.13 - Prob. 1MQCh. 12.13 - How can a tRNA be engineered to encode for a...Ch. 12.13 - Prob. 3MQCh. 12.13 - What are some mechanisms for controlling a...Ch. 12 - Suppose you have just determined the DNA base...Ch. 12 - Prob. 2AQCh. 12 - Prob. 3AQCh. 12 - Describe how you could recode Escherichia coli to...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- What would be an advantage of using HaeIII for a cloning experiment? What would be a disadvantage?arrow_forwardUnneeded genes in an adult animal cell are permanently inactivated,making it impossible for most specialized cells to turn into any othercell type. How does this arrangement save energy inside a cell? Whydoes the ability to clone an adult mammal depend on techniques forreactivating these “dormant” genes?arrow_forwardWhat is a cloning vector? Give two examples of specific DNA molecules routinely used as cloning vectors.arrow_forward
- Besides the great potential of gene editing, what are the biggest risks?  How do scientist test to ensure their gene editing experiments target only the desired specific genes?arrow_forwardWhat is the role of streptomycin in CRISPR experiment? What biochemical changes (DNA, protein) occurred in those cells in which CRISPR worked?arrow_forwardWhat is the difference between manipulating a gene for gene therapy and for producing a transgenic organism?arrow_forward
- How does positional cloning work and What is the basic principle of positional cloning?arrow_forwardFor each of the following experimental goals, is PCR orgene cloning preferable and why?a. Isolate the same gene from 20 individuals.b. Isolate 100 genes from the same individual.c. Isolate a mouse gene when you have a rat genefragment.arrow_forwardCan DNA recombinantion be used to insert human genes into human cells; if so, what are the advanatages of inserting the human gene directly into human cells?arrow_forward
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