(a)
Interpretation:
The absorbance of the solution containing the complex formed between
Concept introduction:
The absorbance of the solution is the ability of the solution to absorb the monochromatic light passing through it. The absorbance of the solution is defined as the ratio of the intensity of light incident on the solution to the intensity of light absorbed by the solution.
The relation between the absorbance, path length, molar absorptivity and the concentration of the solution is given as.
(b)
Interpretation:
The percent transmittance of the solution formed from the complex between
Concept introduction:
The percent transmittance of a solution is the ability of the solution to allow the monochromatic light to pass through it. The percent transmittance of a solution is the ratio of the intensity of monochromatic light incident on the solution to the intensity transmitted through the solution.
The relation between the absorbance and transmittance is established by the Beer’s law.
(c)
Interpretation:
The concentration of the solution is to be determined for the change in the path length of the cell containing the solution.
Concept introduction:
The concentration of the solution, path length of the cell and the molar absorptivity of the solution plays an important role in determining the absorbance of the solution. If any of the parameter is affected, it leads to change in the absorbance of the solution.
The relation between the absorbance, path length, molar absorptivity and the concentration of the solution is given as.
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Chapter 13 Solutions
PRINCIPLES OF INSTRUMENTAL ANALYSIS
- A standard solution was put through appropriate dilutions to give the concentrations of iron shown in the table that follows. The iron(II)-1,10-phenanthroline complex was then formed in 25.0-mL aliquots of these solutions, following which each was diluted to 50.0 mL. The following absorbances (1.00-cm cells) were recorded at 510 nm: Calculate the concentration, in ppm, of a sample with an abosrbance of 0.829. Fe(II) concentration (ppm) A510 4.00 0.160 10.0 0.390 16.0 0.630 24.0 0.950 32.0 1.260 40.0 1.580arrow_forwardA FeSCN complex, whose maximum absorbance is at a λ of 580 nm, has a molar extinction coefficient of 5×103 M-1cm-1 . A solution is measured with a 25 mm cell at the same λ , obtaining 20.6% T. Calculate the concentration in [ppm] of the complex in the solution.arrow_forwardAt 590 nm, crystal violet (a biological stain) has a molar absorptivity (ε) of 8.7×104 M-1cm-1. Using Beer's Law, calculate the concentration of crystal violet in a solution with a measured absorbance (A) of 1.254. The path length (b) is 1.00 cm.arrow_forward
- 7. A solution containing the complex formed between Bi(III) and thiourea has a molar absorptivity of 9.32 x 10° L·cm-1-mol-1 at 470 nm. (a) What is the absorbance of a 6.24 x 10-5 M solution of the complex in a 1.00-cm cell? (b) What is the percent transmittance of the solution described in Part (a)? (c) What is the molarity of the complex in a solution that has the same absorbance as the solution in Part (a), but was measured in a 5.00-cm cell?arrow_forwardThe transmittance of a solution measured at 590 nm in a 1.5-cm cuvette was 76.2%. (a) What is the corresponding absorbance?(b) If the concentration is 0.0802 M, what is the absorptivity of this species at this wavelength? (c) If the absorptivity is 10,000 L mol-1 cm-1, what is the concentration?arrow_forwardA 2.78 ✕ 10−4 M solution of a compound has an absorbance of 0.417 at 520 nm in a 1.00 cm cell. The solvent's absorbance under the same conditions is 0.020. (a) What is the molar absorptivity of the unknown compound? M -1cm-1(b) What is the concentration of the compound in a solution, if the absorbance of the solution in a 1.00 cm cell at 520 nm is 0.391? Marrow_forward
- The absorption coefficient of a glycogen-iodine complex is 0.20 at light of 450 nm. What is the concentration when the transmission is 40 % in a cuvette of 2 cm?arrow_forwardYou are working with metal thiocyanate complex, M(SCN)+, with a molar absorptivity constant, ε, of 2700 cm−1M−1 at 500 nm. If you are is using a 1.00 cm cuvette for absorbance measurements at 500 nm, what concentration of M(SCN)+ will give an absorbance value of 0.280?arrow_forwardMercury(II) forms a 1:1 complex with triphenyltetrazolium chloride that exhibits an absorption maximum at 255 nm. The mercury(II) in a soil sample was extracted into an organic solvent containing an excess of TTC , and the resulting solution was diluted to 100.0 mL in a volumetric flask. Five-milliliter aliquots of the analyte solution were then transferred to six 25-mL volumetric flasks. A standard solution was then prepared that was 5*10^-6 M in . Volumes of the standard solution shown in the table were then pipetted into the volumetric flasks, and each solution was then diluted to 25.00 mL. The absorbance of each solution was measured at 255 nm in 1.00-cm quartz cells. Please solve and explain these questions: A.) Enter the given data pictured above into a spreadsheet and show the correct standard additions plot B.) Determine the slope and the intercept of the line C.)Determine the standard deviation of the slope and the intercept. D.) Calculate the concentration of Hg(II) in the…arrow_forward
- A solution containing a complex formed between thiourea and Bi (III) has a molar absorptivity of 9.32 × 103 L / cm.mol at 470 nm. Questions: A) What is the absorbance of a 5.67 × 10-5 M solution of the complex at 470 nm in a 1.00 cm cell? B) What is the transmittance of the solution? C) What would the concentration be if we have the same absorbance obtained in (a) at 470 nm in a 2.50 cm cell?arrow_forwardThe complex formed between Cu(I) and 1,10- phenanthroline has a molar absorptivity of 7000 L cm-1 mol-1 at 435 nm, the wavelength of maximum absorption. What is the the absorbance of a 6.17 x 10-5 M solution of the complex when measured in a 1.00-cm cell at 435 nm.arrow_forwardAt 580 nm, the wavelength of its maximum absorption, the complex Fe(SCN)2+ has a molar absorptivity of 7.00 X 103 L mol-1 cm-1. Calculate (a) the absorbance of a 2.50 X 10-5 M solution of the complex at 580 nm in a 1.00-cm cell. (b) the absorbance of the solution in which the concentration of the complex is twice that in part (a) (c) the transmittance of the solutions described in parts (a) and (b) (d) the absorbance of a solution that has half the transmittance of that described in part (a) answer letter d pleasearrow_forward
- Principles of Instrumental AnalysisChemistryISBN:9781305577213Author:Douglas A. Skoog, F. James Holler, Stanley R. CrouchPublisher:Cengage Learning