BIOCHEMISTRY
BIOCHEMISTRY
9th Edition
ISBN: 2818440090622
Author: BERG
Publisher: MAC HIGHER
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Chapter 13, Problem 25P
Interpretation Introduction

Interpretation:

In the mechanism of SERCA, whether sustained phosphate production is observed or not needs to be explained.

Concept introduction:

ATP is the energy source of life which is founded in the cells. It works is to supply the cell with energy as well as store the needed energy in the cell. ATP stands for adenosine triphosphate.

SERCA is sarco-endoplasmic reticulum Ca2+ or it is a calcium ATPase. This is a pump that transport calcium from the cytoplasm into SR. SR stands for sarcoplasmic reticulum which helps in regulation of calcium ions in the cytoplasm of striated muscle cells. It occurs both in animal and plant cells.

Calcimycinis a mobile ion carrier which helps in the formation of the divalent complex with divalent cations.

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Fo-F1 ATPase. The energy for ATP synthesis from ADP and Pi is provided by the downhill transport of protons through the rotary FoF1 ATP synthase . The enzyme has 3 alpha-beta and 12 ‘c’ subunits. The mitochondrion maintains change in membrane potential=180 mV (negative inside), pHin = 8, pHout=7, [Pi] = 3 mM and ADP is present as well. . What [ATP]/[ADP] ratio will be established at steady-state under these conditions?  What would be the [ATP]/[ADP] ratio if the enzyme had only 9 ‘c’ subunits? full revolution of the crank (gamma subunit) produces 3 ATP.
Fo-F1 ATPase. The energy for ATP synthesis from ADP and Pi is provided by the downhill transport of protons through the rotary FoF1 ATP synthase (lecture 22).   The enzyme has 3 a-b and 12 ‘c’ subunits. The mitochondrion maintains Df=180 mV (negative inside), pHin = 8, pHout=7, [Pi] = 3 mM and ADP is present as well. How much energy is available (from the proton electrochemical gradient) for ATP synthesis under these conditions (in kJ/mol)?                                                                                                                  What [ATP]/[ADP] ratio will be established at steady-state under these conditions?                                                                                                                                                What would be the [ATP]/[ADP] ratio if the enzyme had only 9 ‘c’ subunits? Remember that full revolution of the crank (gamma subunit) produces 3 ATP.
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