Biochemistry (Looseleaf)
9th Edition
ISBN: 9781319114800
Author: BERG
Publisher: MAC HIGHER
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Question
Chapter 13, Problem 29P
Interpretation Introduction
Interpretation:
The effect of the mutation on channel function needs to be explained.
Concept introduction:
Acetylcholine act as a neurotransmitter which means it sends messages or chemicals from one cell to another cell of the body. Acetylcholinesterase helps in stopping the excitation of a nerve cell after the transmission of impulse or in other words, one can say that this enzyme causes rapid hydrolysis of acetylcholine.
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Enzyme Kinetics question
Enzyme used is 10uL of a 10 ng/uL solution to a reaction mix in a final volume of 2.0 mL. (Enzyme used is 20kDa monomeric enzyme if matters)
Based off lineweaver burk where 300 uM of inhibitor is used
noninhibited formula is y= 4x + 0.1 (x axis is 1/S 1/mM) (y axis is 1/Vo sec/mM)
inhibited formula is y = 4x + 1
I found Km as 1 mM for inhibited
Vmax as 1mM/sec for inhibited
How would I find Kcat?
How would I find Ki?
Need help, please.
Chapter 13 Solutions
Biochemistry (Looseleaf)
Ch. 13 - Prob. 1PCh. 13 - Prob. 2PCh. 13 - Prob. 3PCh. 13 - Prob. 4PCh. 13 - Prob. 5PCh. 13 - Prob. 6PCh. 13 - Prob. 7PCh. 13 - Prob. 8PCh. 13 - Prob. 9PCh. 13 - Prob. 10P
Ch. 13 - Prob. 11PCh. 13 - Prob. 12PCh. 13 - Prob. 13PCh. 13 - Prob. 14PCh. 13 - Prob. 15PCh. 13 - Prob. 16PCh. 13 - Prob. 17PCh. 13 - Prob. 18PCh. 13 - Prob. 19PCh. 13 - Prob. 20PCh. 13 - Prob. 21PCh. 13 - Prob. 22PCh. 13 - Prob. 23PCh. 13 - Prob. 24PCh. 13 - Prob. 25PCh. 13 - Prob. 26PCh. 13 - Prob. 27PCh. 13 - Prob. 28PCh. 13 - Prob. 29PCh. 13 - Prob. 30PCh. 13 - Prob. 31PCh. 13 - Prob. 32P
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