Genetic Analysis: An Integrated Approach (2nd Edition)
2nd Edition
ISBN: 9780321948908
Author: Mark F. Sanders, John L. Bowman
Publisher: PEARSON
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Chapter 13, Problem 30P
After reading Experimental Insight
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Prototrophy is often the phenotype selected to detecttransformants. Prototrophic cells are used for donorDNA extraction; then this DNA is cloned and the clonesare added to an auxotrophic recipient culture. Successfultransformants are identified by plating the recipientculture on minimal medium and looking for colonies.What experimental design would you use to make surethat a colony that you hope is a transformant is not, infact,a. a prototrophic cell that has entered the recipientculture as a contaminant?b. a revertant (mutation back to prototrophy by a secondmutation in the originally mutated gene) of theauxotrophic mutation?
Time mapping is performed in a cross involving the genes his,leu, mal, and xyl. The recipient cells are auxotrophic for all fourgenes. After 25 minutes, mating is interrupted, with the resultsin recipient cells shown below. Diagram the positions of thesegenes relative to the origin (O) of the F factor and to one another.(a) 90% are xyl+(b) 80% are mal+(c) 20% are his+(d) None are leu+
What would be the most likely effect of deleting flowering locus D (FLD) in Arabidopsis thaliana?
Chapter 13 Solutions
Genetic Analysis: An Integrated Approach (2nd Edition)
Ch. 13 - Prob. 1PCh. 13 - For one set of chromosomes carried by a triploid...Ch. 13 - 10.8 If the haploid number for a plant species is...Ch. 13 - From the following list, identify the types...Ch. 13 - Mating between a male donkey (2n=64) and a female...Ch. 13 - Prob. 6PCh. 13 - Prob. 7PCh. 13 - What can we conclude about a mutational event...Ch. 13 - 9. In terms of the chromosome content of nuclei,...Ch. 13 - 10. In Drosophila, an X-linked recessive allele...
Ch. 13 - The plants in this problem are the same as those...Ch. 13 - A normal chromosome and its homolog carrying a...Ch. 13 - 10.12 A pair of homologous chromosomes in...Ch. 13 - 10.13 An animal heterozygous for a reciprocal...Ch. 13 - Dr. Ara B. Dopsis has an idea he thinks will be a...Ch. 13 - Suppose polymerase chain reaction (PCR) is used...Ch. 13 - Prob. 17PCh. 13 - A healthy couple with a history of three previous...Ch. 13 - A boy with Down syndrome (trisomy 21) has 46...Ch. 13 - Prob. 20PCh. 13 - 10.28 A small population of deer living on an...Ch. 13 - In humans that XX/XO mosaics, the phenotype is...Ch. 13 - 10.20 A plant breeder would like to develop the...Ch. 13 - In Drosophilia, seven partial deletion (1to7)...Ch. 13 - Two experimental varieties of strawberry are...Ch. 13 - 10.23 In the tomato, Solanum esculentum, tall ()...Ch. 13 - In Drosophila, the wild-type red eye color is...Ch. 13 - A DrosophilaPelement 2.5kb in length is modified...Ch. 13 - A biologist studying flight mechanisms in insects...Ch. 13 - 30. After reading Experimental Insight and...Ch. 13 - 31. Two NotI restriction enzymes cleave DNA on...Ch. 13 - For the following crosses, determine as accurately...
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- Consider two maize plants:a. Genotype C/cm ; Ac/Ac+, where cm is an unstableallele caused by a Ds insertionb. Genotype C/cm, where cm is an unstable allele causedby Ac insertionWhat phenotypes would be produced and in whatproportions when (1) each plant is crossed with a basepair-substitution mutant c/c and (2) the plant in part a iscrossed with the plant in part b? Assume that Ac and care unlinked, that the chromosome-breakage frequencyis negligible, and that mutant c /C is Ac+.arrow_forwardExamine the trait distribution of Specific Leaf Area (The ratio of leaf area to dry mass, SLA), below. The dotted normal distribution represents the natural pattern of SLA observed in nature. The histogram below the dotted curve represents the distribution of phenotypes when the plant is grown in a single common environment. The numbers above the bars represent the observed phenotype ratio. a a) How many polygenes encode for this trait? b) Assuming the minimum observed phenotype in a common garden was 8sqm/kg and the maximum observed phenotype was 20 sqm/kg, what is the contribution of each allele in this polygene?arrow_forwardIn the chapter-opening photograph of kernels on an earof corn, what is the genetic basis of the following (Hint:Refer to Figure 15-4 for some clues):a. the fully pigmented kernel?b. the unpigmented kernels? Note that they can arise intwo different ways.arrow_forward
- Neurospora of genotype a + c are crossed withNeurospora of genotype + b +. (Here, + is shorthandfor the wild-type allele.) The following tetrads areobtained (note that the genotype of the four sporepairs in an ascus are listed, rather than listing alleight spores):a + c a b c + + c + b c a b + a + ca + c a b c a + c a b c a b + a b c+ b + + + + + b + + + + + + c + + ++ b + + + + a b + a + + + + c + b +137 141 26 25 2 3a. In how many cells has meiosis occurred to yieldthese data?b. Give the best genetic map to explain these results.Indicate all relevant genetic distances, both betweengenes and between each gene and the centromere.c. Diagram a meiosis that could give rise to oneof the three tetrads in the class at the far right inthe listarrow_forwardWhat three essential criteria must be met in order to execute asuccessful mapping cross?arrow_forwardExplain the similarities and differences between the procedures used for the coarse-scale mapping and fine-scale mapping of QTLs through controlled crosses, as was accomplished for QTLs involved with tomato size.arrow_forward
- Time mapping is performed in a cross involving the genes his, leu, mal, and xyl. The recipient cells were auxotrophic for all four genes. After 25 minutes, mating was interrupted with the following results in recipient cells. Diagram the positions of these genes relative to the origin (O) of the F factor and to one another. (a) 90% were xyl+ (b) 80% were mal+ (c) 20% were his+ (d) none were leu+arrow_forwardF1 hybrids between two species of cotton, Gossypium barbadenseand Gossypium hirsutum, are very vigorous plants. However, F1crosses produce many seeds that do not germinate and a high percentageof very weak F2 offspring. Suggest two reasons for theseobservations.arrow_forwardGene mapping using the Three-point Testcross a) Given the following alleles that control seed traits:W = wrinkled G = green R = roundw = smooth g = yellow r = oval b) Results of a cross with a triple heterozygote revealed the following phenotypes:30 smooth yellow round4 smooth green round958 wrinkled green round2 wrinkled yellow oval18 wrinkled yellow round946 smooth yellow oval16 smooth green oval26 wrinkled green oval c) Determine the order of the genes and the distance between them in centiMorgan (cM).Construct a gene map to show your results. TIP: Based on the phenotypes, determine the alleles in the gametesarrow_forward
- Because snapdragons (Antirrhinum) possess the pigment anthocyanin, they have reddish purple petals.Two pure anthocyaninless lines of Antirrhinum weredeveloped, one in California and one in Holland. Theylooked identical in having no red pigment at all, manifested as white (albino) flowers. However, when petalsfrom the two lines were ground up together in buffer inthe same test tube, the solution, which appeared colorless at first, gradually turned red.a. What control experiments should an investigatorconduct before proceeding with further analysis?b. What could account for the production of the redcolor in the test tube?c. According to your explanation for part b, what wouldbe the genotypes of the two lines?d. If the two white lines were crossed, what would youpredict the phenotypes of the F1 and F2 to be?arrow_forwardIn the Western blot shown here, proteins were isolated from redblood cells and muscle cells from two different individuals. Oneindividual was unaffected, and the other suffered from a diseaseknown as thalassemia, which involves a defect in hemoglobin. Theblot was exposed to an antibody that recognizes β globin, whichis one of the polypeptides that constitute hemoglobin. Equal totalamounts of cellular proteins were added to each lane. Explain these results.arrow_forwardDoes this statement is a example of a phenocopy? Explain your reasoning. Q. Dwarf plants and mottled leaves in tomatoes are caused by separate genes that are linked.arrow_forward
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