Genetic Analysis: An Integrated Approach (2nd Edition)
2nd Edition
ISBN: 9780321948908
Author: Mark F. Sanders, John L. Bowman
Publisher: PEARSON
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Chapter 13, Problem 29P
A biologist studying flight mechanisms in insects wantsto introduce a dominant mutant allele producing oversized wings, called flapper, into the Drosophilagenome. The biologist chooses a strain of fruit fly homozygous fora recessive mutant producing miniature wings. How will the biologist design the experiment using a Pelement todeliver the mutant allele to the genome?
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You are working in the lab with strains of Drosophila that have either normal legs or abnormally short legs and you are studying the gene responsible. You know that normal legs are dominant to short legs. You come across a misplaced fly with normal legs, but you are not sure of his genetic background and you want to keep him in your experiments.
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Chapter 13 Solutions
Genetic Analysis: An Integrated Approach (2nd Edition)
Ch. 13 - Prob. 1PCh. 13 - For one set of chromosomes carried by a triploid...Ch. 13 - 10.8 If the haploid number for a plant species is...Ch. 13 - From the following list, identify the types...Ch. 13 - Mating between a male donkey (2n=64) and a female...Ch. 13 - Prob. 6PCh. 13 - Prob. 7PCh. 13 - What can we conclude about a mutational event...Ch. 13 - 9. In terms of the chromosome content of nuclei,...Ch. 13 - 10. In Drosophila, an X-linked recessive allele...
Ch. 13 - The plants in this problem are the same as those...Ch. 13 - A normal chromosome and its homolog carrying a...Ch. 13 - 10.12 A pair of homologous chromosomes in...Ch. 13 - 10.13 An animal heterozygous for a reciprocal...Ch. 13 - Dr. Ara B. Dopsis has an idea he thinks will be a...Ch. 13 - Suppose polymerase chain reaction (PCR) is used...Ch. 13 - Prob. 17PCh. 13 - A healthy couple with a history of three previous...Ch. 13 - A boy with Down syndrome (trisomy 21) has 46...Ch. 13 - Prob. 20PCh. 13 - 10.28 A small population of deer living on an...Ch. 13 - In humans that XX/XO mosaics, the phenotype is...Ch. 13 - 10.20 A plant breeder would like to develop the...Ch. 13 - In Drosophilia, seven partial deletion (1to7)...Ch. 13 - Two experimental varieties of strawberry are...Ch. 13 - 10.23 In the tomato, Solanum esculentum, tall ()...Ch. 13 - In Drosophila, the wild-type red eye color is...Ch. 13 - A DrosophilaPelement 2.5kb in length is modified...Ch. 13 - A biologist studying flight mechanisms in insects...Ch. 13 - 30. After reading Experimental Insight and...Ch. 13 - 31. Two NotI restriction enzymes cleave DNA on...Ch. 13 - For the following crosses, determine as accurately...
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- Suppose a researcher has three different Drosophila strains that have mutations in the bicoid gene called bicoid-A, bicoid-B, and bicoid-C; the wild type is designated bicoid +. To study these mutations, phenotypically normal female flies that are homozygous for the given bicoid mutation were obtained, and their oocytes were analyzed using a Northern blot to determine the size and/or amount of the bicoid mRNA and in situ hybridization to determine the bicoid mRNA location within the oocyte. A wild-type strain was also analyzed as a control. In both cases, the probe was complementary to the bicoid mRNA and the results are shown below. (Anterior is on the left; posterior is on the right.) Northern blot 1 2 - 3 4 In situ hybridization Wild type Lane 1. Wild type (bicoid*) Lane 2. bicoid-A Lane 3. bicoid-B Lane 4. bicoid-C bicoid-B bicoid-A bicoid-C Which mutation is likely to cause the embryo to develop two "anterior" ends? bicoid-B Obicoid-A bicoid-Carrow_forwardFor the first experiment ever on Drosophila mutations. Answer the following questions. a. What is the title of the first published paper explained the experiment and what is the name of the Author? b. What is the first mutation discovered in Drosophila? c. Explain the changes in the Drosophila yellow mutant (Y)compared to wild type.arrow_forwardGiven that you have an even-skipped mutant (Drosophila) which results in the loss of stripe #5, describe an experiment that you could devise to reintroduce/rescue the mutation.arrow_forward
- In Drosophila, a heterozygous female for the X-linkedrecessive traits a, b, and c was crossed to a male that phenotypically expressed a, b, and c. The offspring occurred inthe following phenotypic ratios.+ b c 460a + + 450a b c 32+ + + 38a + c 11+ b + 9 No other phenotypes were observed.(a) Determine the correct sequence and construct amap of these genes on the X chromosome ?arrow_forwardYou ave established a mutant line of flies with a balancer chromosome. The balancer chromosome includes GFP and a mutation in gene X that causes flies to be sterile in the homozygous state. The recessive mutation that you are studying is in gene Y, and causes flies to develop two tail ends in the homozygous state. This mutation is lethal in the homozygous state. For each of the following phenotypes in the balanced mutant line, what alleles are present and in how many copies? a.) Glows green, fertile (Select] b.) Glows green, sterile [Select) c.) does not glow green, two tails (Select] If you crossed a fly from your balanced line with the following flies, what proportion of the offspring would glow green and be fertile? d.) wild-type [Select ) e.) fly with balancer chromosome from parental generation [ Select ]arrow_forwarddescribe two blotting methods (i.e., Northern blottingand Western blotting) used to detect gene products. Northern blotting detects RNA and Western blotting detects proteins. Supposethat a female fruit fly is heterozygous for a maternal effect gene,which we will call gene B. The female is Bb. The normal allele, B,encodes a functional mRNA that is 550 nucleotides long. A recessive allele, b, encodes a shorter mRNA that is 375 nucleotideslong. (Allele b is due to a deletion within this gene.) How couldyou use one or more of these techniques to show that nurse cellstransfer gene products from gene B to developing oocytes? Youmay assume that you can dissect the ovaries of fruit flies and isolate oocytes separately from nurse cells. In your answer, describeyour expected results.arrow_forward
- You are a developmental geneticist studying flowering time variation in Arabidopsis. You perform a mutagenesis screen to identify mutants in the photoperiod pathway. You conduct the screen and find two different plants that show the same mutant phenotype. You then use a complementation test. What is the predicted outcome of this test if both phenotypes are caused by mutations in separate genes? recover the wild type phenotype overexpress the gene O recover the mutant phenotypearrow_forwardAn undergraduate researcher in your lab is studying mutations affecting the wings of Drosophila melanogaster. She has identified two mutant phenotypes of interest: bent wings (be), which are recessive to the wild-type straight wings (be+), and apterous (ap) mutants (which are wingless). The apallele is recessive to the wild-type allele (ap+), which allows wings to develop. If a homozygous bent-winged fly (which possesses the normal allele of apterous) is crossed with a homozygous wingless fly (which possesses the normal allele of bent wings), what phenotypic ratio would you expect to observe in the F2 generation of this cross? a) Please indicate the ratio, including the genotypes and phenotypes of all phenotypic classes. Phenotype: Genotype(s) corresponding to this phenotype Phenotypic ratio: (Be sure to NAME the classes in the ratio). B) Please NAME and DEFINE the type of gene interaction illustrated in this example.arrow_forwardDraw a basket mutant embryo. What does basket encode? Why do the mutant embryos have this phenotype?arrow_forward
- The mutations called bobbed in Drosophila result from variable reductions (deletions) in the number of amplified genes coding for rRNA. Researchers trying to maintain bobbed stocks have often documented their tendency to revert to wild type in successive generations. Propose a mechanism based on meiotic recombination which could account for this reversion phenomenon. Why would wild-type flies become more prevalent in Drosophila cultures?arrow_forwardDrosophila researchers have collected many strainsthat carry a single recombinant P element containing awild-type white gene (a P[w+] transgene) inserted intoa known genomic location. These strains can be used tomap the location of any mutant gene in the fly genome.Investigators performed a testcross to map arecessive mutation rough (ro), which causes rougheyes, relative to a P[w+] element on chromosome 3.Females heterozygous for the P[w+] on one chromosome 3 and a ro− mutation on the other, homologouschromosome 3 were crossed to ro−/ro− males, and theprogeny in the following list were obtained. In boththe parents and the progeny, the endogenous whitegene is nonfunctional—the flies have red eyes onlyif they contain the P[w+] transgene.145 red, smooth (wild-type) eyes152 white, rough eyes2 white, smooth eyes1 red, rough eyesa. Are ro and the P[w+] linked? If so, how many mapunits separate them?b. The data in part (a) do not indicate on which sideof the P[w+] (toward the centromere or…arrow_forwardSuppose that a geneticist discovers a new mutation in Drosophila melanogaster that causes the flies to shake and quiver. She calls this mutation quiver, qu, and determines that it is due to an autosomal recessive gene. She wants to determine whether the gene encoding quiver is linked to the recessive gene for vestigial wings, vg. She crosses a fly homozygous for quiver and vestigial traits with a fly homozygous for the wild-type traits, and then uses the resulting F1 females in a testcross. She obtains the flies from this testcross. Phenotype Number of flies vg+ qu+ 230 vg qu 224 vg qu+ 97 vg* qu 99 Test the hypothesis that the genes quiver and vestigial assort independently by calculating the chi-squared, X², for this hypothesis. Provide the X2 to one decimal place. X2 Does the X2 value support the hypothesis that the quiver and vestigial genes assort independently? Why the partial table of critical values for X2 calculations to test this hypothesis. or why not? Use O No, the X' value…arrow_forward
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