The radioactive (labeled) and the nonradioactive (non-labeled) nucleotides , present in the double-stranded deoxyribonucleic acid (DNA) that has undergone pulse and chase process with the radioactive thymidine. Introduction: The DNA is duplicated in the S (synthesis) phase of the cell cycle. The duplication process can be traced by detecting the incorporation of the radioactively labeled thymidine in the DNA of the S phase cells.

Question

Want to see the full answer?

Answer 1

Question

Chapter 13, Problem 4Q

Summary Introduction

To explain:

The radioactive (labeled) and the nonradioactive (non-labeled) nucleotides, present in the double-stranded deoxyribonucleic acid (DNA) that has undergone pulse and chase process with the radioactive thymidine.

Introduction:

The DNA is duplicated in the S (synthesis) phase of the cell cycle. The duplication process can be traced by detecting the incorporation of the radioactively labeled thymidine in the DNA of the S phase cells.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Students have asked these similar questions

Consider the experiment conducted by Meselson and Stahl in which they used 14N and 15N in cultures of E. coli and equilibrium density gradient centrifugation. Draw pictures to represent the bands produced by bacterial DNA in the centrifuge tube before the switch to medium containing 14N and after one, two, and three rounds of replication in that medium. Use separate sets of drawings to show the bands that would appear if replication were (a) semiconservative; (b) conservative; (c) dispersive.

Use a drawing to illustrate the principle of DNA gel electrophoresis. (2 marks)-+

The table below summarises the three stages of Meselson and Stahl's experiment and their results. (a) Complete the table by drawing, in the appropriate boxes, diagrams of the DNA molecules and mark the position and size of the DNA bands in the tubes. Experimental stage Diagram to show the strands in the DNA Position and size of DNA bands in the tube of molecules of the bacteria separating solution Stage 1 Bacteria grown for several generations in culture medium containing heavy nitrogen Stage 2 The bacteria from the end of stage 1 were grown for another generation in culture medium containing light nitrogen TA2G - Completed forms must be available for Open Awards extermal moderation purposes. Page 7 of 13 Stage 3 The bacteria from the end of stage 2 were grown for one more generation in culture medium containing light nitrogen (b) The bacteria at the end of stage two were grown for five more generations. After each generation, what would you expect to see in the test tube? Draw these…