To analyze:
In the hybrid dysgenesis of D. melanogaster, it shows the transposition repressor coded by the P element.
This repressor limits the number of P elements in a genome.
What is the advantage of limiting the P elements in the genome?
Introduction:
Transposition is the movement of transposable elements from one site to another.
These are DNA sequences having various sizes and sequence arrangement. They move throughout the genome to increase their own copy number. In the
P
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GENETIC ANALYSIS: AN INTEG. APP. W/MAS
- The MAT locus allows yeast to switch mating type through a very complex mechanism. However, it has informed us a great deal about what aspects of gene expression typical to all organisms? Options: higher order changes in chromatin affect transcriptional efficiency that general transcription factors must first bind directly to histone tails and only then can they interact with their cognate binding sites that DNA methylation is involved in this silencing mechanism that SIR2 is required for all types of transcriptional repression that expression of Pol III genes provides a means of identifying active chromatinarrow_forwardContrast the role of the MCM1 protein in different yeastcell types shown in Figure 12-10. How are the a-specificgenes controlled differently in different cell types?arrow_forwardRecall that the nuclear membrane disintegrates late in prophase of mitosis in most eukarvotic cells. Once the membrane is reformed in telophase in a daughter cell, several components of gene expression mignt therefore be "caught" out in the cytoplasm when they would otherwise onlv ever be found inside the nucleus. Consider where the following components of gene expression are made and where they runction. Which component is normally never found in the cytoplasm outside the nucleus? O A. Spliced intron • B. RNA polymerase O C. Histones • D. DNA polymerasearrow_forward
- Expression of recombinant proteins in yeast is an important tool for biotechnology companies that produce new drugs for human use. In an attempt to get a new gene X expressed in yeast, a researcher has integrated gene X into the yeast genome near a telomere. Will this strategy result in good expression of gene X? Why or why not? please try to explain a bit elaborately.arrow_forwardBelow is a schematic diagram showing a 3000 bp region of yeast genomic DNA. TSS 5' 3' 5' +1 (i) Draw and name specific regions in this schematic diagram that can be recognized by Transcription factor IID (TFIID). (ii) How does Transcription factor IIH (TFIIH) initiate the transcription? in inarrow_forwardA new gene X expressed in yeast, a researcher has integrated gene X into the yeast genome near a telomere. Will this strategy result in the good expression of gene X? Why?arrow_forward
- expression of recombinant proteins in yeast is an important tool for biotechnology companies that produce new drugs for human use.in an attempt to get a new gene X expressed in yeast, a researcher has integrated gene X into the yeast genome near a telomere. will this strategy result in good expression of gene X? why or why not?arrow_forwardIn a haploid, a nonsense suppressor su1 acts on mutation1 but not on mutation 2 or 3 of gene P. An unlinked nonsense suppressor su2 works on P mutation 2 but not on 1or 3. Explain this pattern of suppression in regard to thenature of the mutations and the suppressors.arrow_forwardDescribe how P elements are used to producetransgenic Drosophila.arrow_forward
- Gunter Korge examined several proteins that are secreted from the salivary glands of Drosophila melanogaster during larval development . One protein, called protein fraction 4, was encoded by a gene found by deletion mapping to be located on the X chromosome at position 3C. Korge observed that, about 5 hours before the first synthesis of protein fraction 4, an expanded and puffed-out region formed on the X chromosome at position 3C. This chromosome puff disappeared before the end of the third larval instar stage, when the synthesis of protein fraction 4 ceased. He observed that there was no puff at position 3C in a special strain of flies that lacked secretion of protein fraction 4. Explain these results. What is the chromosome puff at region 3, and why does its appearance and disappearance roughly coincide with the secretion of protein fraction 4?arrow_forwardIn the Fast Forward Box Visualizing X ChromosomeInactivation in Transgenic Mice, suppose the investigators had looked at the expression of green and red fluorescent protein in early mouse embryos, when theembryos have fewer than 500 cells. What patterns wouldthey likely have observed? (Assume that the transgenesmake gene product this early in development.)arrow_forwardDiscuss how ultra violet light works as a mutagen. This could include: What is UV light and the Mutations commonly introduced by UV light? What are the Repair mechanisms in yeast that fix damage caused by UV light? Describe the phenotypes of Saccharomyces Cerevisiae plates, the first plate is the control yeast, while the second plate has been exposed to low UV light, the third plate has been exposed to high UV light. Normal yeast has round smooth white colonies. Are there any signinifcant differences?arrow_forward
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