Microbiology with Diseases by Taxonomy Plus Mastering Microbiology with Pearson eText -- Access Card Package (5th Edition)
5th Edition
ISBN: 9780133948851
Author: Robert W. Bauman Ph.D.
Publisher: PEARSON
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Chapter 13, Problem 9CT
Summary Introduction
To determine:
How many replication cycles occurs for T4 phages which produced 200 new phages to overwhelm the entire bacterial colony.
Introduction:
Replication is defined as a biological process which involves production of two identical copies of DNA from one original DNA molecule. It occurs in all living organisms. It serves basis for biological inheritance.
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In a petri dish with solidified agar with escherichia coli, enterobacter aerogenes and staphylococcus aureus, you streak a loopfull of lytic T4-phage in a single line onto the center of the the dish, how do you know if bacteriophage infected the bacteria.
why didn't the bacteriophage infect all 3 bacteria?
Why are prokaryotes able to replicate so much more quickly than eukaryotes? What cellular components must be copied before a cell splits in two?
Why is this difference in replication rate an important part of our vulnerability to bacterial pathogens?
Which statement describes the difference between a lytic and lysogenic phage cycle?
In a lytic cycle, new phage particles will be produced; in a lysogenic cycle, the phage DNA is passed to new cells but does not produce phage particles.
In a lytic cycle, the host cell does not die; in a lysogenic cycle, the host is killed.
In a lytic cycle, the phage DNA enters the host cell; in a lysogenic cycle, the phage DNA remains on the exterior of the host cell.
In a lytic cycle, the phage DNA incorporates into the host genome; in a lysogenic cycle, the phage DNA does not incorporate into the host genome.
Chapter 13 Solutions
Microbiology with Diseases by Taxonomy Plus Mastering Microbiology with Pearson eText -- Access Card Package (5th Edition)
Ch. 13 - Prob. 1MCCh. 13 - Which of the following statements is true? a....Ch. 13 - Prob. 3MCCh. 13 - Prob. 4MCCh. 13 - Prob. 5MCCh. 13 - Prob. 6MCCh. 13 - Prob. 7MCCh. 13 - Prob. 8MCCh. 13 - Which of the following is not a criterion for...Ch. 13 - Prob. 10MC
Ch. 13 - Match each numbered term with its description. 1....Ch. 13 - Prob. 1VICh. 13 - Prob. 2VICh. 13 - Compare and contrast a bacterium and a virus by...Ch. 13 - Prob. 2SACh. 13 - Prob. 3SACh. 13 - Prob. 4SACh. 13 - Prob. 5SACh. 13 - Prob. 6SACh. 13 - Prob. 7SACh. 13 - Prob. 8SACh. 13 - Prob. 9SACh. 13 - Compare and contrast diploid cell culture and...Ch. 13 - Larger viruses usually have a double-stranded...Ch. 13 - What are the advantages and disadvantages to...Ch. 13 - How are computer viruses similar to biological...Ch. 13 - Compare and contrast lysogeny by a prophage and...Ch. 13 - Prob. 5CTCh. 13 - Prob. 6CTCh. 13 - Prob. 7CTCh. 13 - Prob. 8CTCh. 13 - Prob. 9CTCh. 13 - What differences would you expect in the...Ch. 13 - Prob. 11CTCh. 13 - Prob. 12CTCh. 13 - Prob. 13CTCh. 13 - Using the following terms, fill in the following...Ch. 13 - Why are naked icosahedral viruses able to...Ch. 13 - What characteristics of the genomes of...Ch. 13 - Prob. 3TMWCh. 13 - Prob. 4TMWCh. 13 - Prob. 5TMWCh. 13 - Why are viruses seemingly alive and yet not alive?Ch. 13 - Prob. 7TMWCh. 13 - Prob. 1EDCSCh. 13 - Prob. 1CCS
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- Why are prokaryotes able to replicate so much more quickly than eukaryotes? (Hint: What cellular components must be copied before a cell splits in two?) Why is this difference in replication rate an important part of our vulnerability to bacterial pathogens?arrow_forwardConsider a hypothetical phage whose DNA replicates exclusively by rolling circle replication. A phage with radioactive DNA in both strands infects a bacterium and is allowed to replicate in a nonradioactive medium. Assume that only daughter DNA from the elongated branch ever gets packaged into progeny particles.  What fraction of the parental radioactivity will appear in the progeny phage? How many progeny phage will contain radioactive DNA? What is the fundamental difference between the initiation of θ replication and that of the rolling circle?arrow_forwardMost temperate phages integrate into the host chromosome, whereas some replicate as plasmids. Which kind of relationship would you think would be more likely to maintain the phage in the host cell? Why?arrow_forward
- Assume you isolate a single stranded (+) RNA virus. When you examine the proteins in the virus, you find that it does NOT contain replicase enzymes within its capsid. Which of the following is true? This virus must have a gene that encodes replicase. This virus will not be able to enter a host cell. Its genome cannot be translated (the process of translation) by the host cell ribosomes. A DNA copy of the viral genome has to be made before viral genes are expressed. This virus must lack surface antigens.arrow_forwardWhy is a webbed plate capable of producing a higher lysate titer (pfu/mL) than a cleared plate? a.) A webbed plate has plaques that have gone through more rounds of replication because the number of host cells did not limit phage replication, creating exponentially more phage particles b.) A webbed plate has no more host available for replication, meaning the plate is full of exponentially higher amounts of phage c.) A cleared plate has less bacteria than a webbed plate, so there are more plaques present d.) A cleared plate contains more phage than a webbed plate because it started with a more concentrated phage samplearrow_forwardImagine that you are a student in Alfred Hershey and Martha Chase’s lab in the late 1940s. You are given five test tubes containing E. Coli bacteria infected with T2 bacteriophages that have been labeled with either 32P or 35S. Unfortunately, you forget to mark the tubes and are now uncertain about which tubes is which. You performed their blender experiment and got the following results. Which tube out of these 5 contains E. Coli infected with 32P-labeled phage? Explain your answer.arrow_forward
- In Hershey-Chase experiment, bacteriophages protein coats were tagged with radioactive isotope S-32. These phages were used to infect E. coli cells and the cells were further centrifuged to form pellets. Why was the radioactivity level of S-32 found greater outside the cells compared to the E. coli cell pellets? Explain briefly. If the experiment is repeated in the same manner but this time the phage protein coats are labelled with isotope X and the phage DNA with isotope Y, which isotope’s radioactivity will be found in greater amounts in the E. coli cell pellets after centrifugation? Explain briefly.arrow_forwardWith respect to the replication strategy, what unusual feature does HBV have in common with HIV?arrow_forwardAfter a phage injects its DNA into a bacterial cell, the cell begins making proteins that make up the phage coat. Why does the same thing not happen when a generalized transducing particle injects the DNA it carries?arrow_forward
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