Genetic Analysis: An Integrated Approach (2nd Edition)
2nd Edition
ISBN: 9780321948908
Author: Mark F. Sanders, John L. Bowman
Publisher: PEARSON
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Textbook Question
Chapter 14, Problem 9P
What role does cAMP play in transcription of lac operon genes? What role does CAP play in transcription of lac operon genes?
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Chapter 14 Solutions
Genetic Analysis: An Integrated Approach (2nd Edition)
Ch. 14 - 12.1 Bacterial genomes frequently contain groups...Ch. 14 - Transcriptional regulation of operon gene...Ch. 14 - Why is it essential that bacterial cells be able...Ch. 14 - Identify similarities and differences between an...Ch. 14 - The transcription of -galactosidase and permease...Ch. 14 - 12.6 Is attenuation the product of an allosteric...Ch. 14 - The trpL region contains four repeated DNA...Ch. 14 - The CAP binding site in the lac promoter is the...Ch. 14 - What role does cAMP play in transcription of lac...Ch. 14 - How would a cap- mutation that produces an...
Ch. 14 - Explain the circumstances under which attenuation...Ch. 14 - Consider the transcription of genes of the...Ch. 14 - Describe the lytic and lysogenic life cycles of ...Ch. 14 - 12.14 Define antisense RNA, and describe how it...Ch. 14 - 12.15 Attenuation of trp operon transcription is...Ch. 14 - 12.16 In the lac operon, what are the likely...Ch. 14 - Identify which of the following lac operon haploid...Ch. 14 - Prob. 18PCh. 14 - 12.19 List possible genotypes for lac operon...Ch. 14 - Suppose each of the genotypes you listed in parts...Ch. 14 - 12.21 Four independent mutants (mutants A to D)...Ch. 14 - Suppose the lac operon partial diploid...Ch. 14 - Prob. 23PCh. 14 - 12.24 A repressible operon system, like the trp...Ch. 14 - 12.25 What is the likely effect of each of the...Ch. 14 - 12.26 Suppose that base substitution mutations...Ch. 14 - 12.27 Two different mutations affect. Mutant...Ch. 14 - How would mutations that inactivate each of the...Ch. 14 - The bacterial insertion sequence IS 10 uses...Ch. 14 - 12.34 Northern blot analysis is performed on...Ch. 14 - 12.37 The electrophoresis gel shown in part (a) is...Ch. 14 - Prob. 32PCh. 14 - The following hypothetical genotypes have genes A,...Ch. 14 - For an E. coli strain with the lac operongenotype...
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- What is the difference between positive regulation of the lac operon and negative regulation of the lac operon?arrow_forwardWhat is lac operon? Draw and/or identify the status of the lac operon in a given set of environmental conditions and/or cell mutations.arrow_forwardHow does catabolite repression work on gal operon?How could you decide if one particular operon is under same catabolite repression as that for lac and gal operons.arrow_forward
- What is the function of the Z gene of the Lac operon?arrow_forwardA bacterial cell is mutated and cannot create the repressor protein associated with the Lac operon. How will this affect the functioning of the Lac operon?arrow_forwardWhat does the lac operon consist of? How is the operator switch turned on and off in the expression of genes in this operon? Explain.arrow_forward
- In the lac operon system, do the inducers act at the transcription or translation level? Explain why it acts in that level in a short but concise way.arrow_forwardHow does repression work in the lac operon?arrow_forwardHow does catabolite repression affect expression of the lac operon and how can it lead to diauxic growth? What role do CRP and cyclic AMP play? Predict what would happen metabolically if you grew E. coli up in a medium containing both glucose and lactose.arrow_forward
- If glucose levels in the cell are high and lactose is available from the environment, what is the status of transcription of the lac operon genes? Explain your answer from both an evolutionary perspective and in terms of negative and positive regulation of the operon?arrow_forwardWhat is lac operon?arrow_forward
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