Connect With Learnsmart Labs Online Access For Prescott's Microbiology
11th Edition
ISBN: 9781260408997
Author: Joanne Willey
Publisher: Mcgraw-hill Higher Education (us)
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Chapter 14.5, Problem 1MI
MICRO INQUIRY
Relative to each promoter, where would you predict phosphorylated OmpR would bind the ompC and ompF genes?
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E30. An electrophoretic mobility shift assay can be used to study the
binding of proteins to a segment of DNA. In the experiment shown
here, an EMSA was used to examine the requirements for the bind-
ing of RNA polymerase II (from eukaryotic cells) to the promoter
of a protein-encoding gene. The assembly of general transcription
factors and RNA polymerase II at the core promoter is described
in Chapter 12 (Figure 12.14). In this experiment, the segment of
DNA containing a promoter sequence was 1100 bp in length. The
fragment was mixed with various combinations of proteins and
then subjected to an EMSA.
Lane 1: No proteins added
Lane 2: TFID
Lane 3: TFIIB
Lane 4: RNA polymerase I|
Lane 5: TFID + TFIIB
Lane 6: TFID + RNA
1 2
4 5 6
polymerase II|
Lane 7: TFIID +
TFIIB + RNA
polymerase I|
1100 bp
Explain which proteins (TFIID, TFIIB, or RNA polymerase II) are
able to bind to this DNA fragment by themselves. Which transcrip-
tion factors (i.e., TFIID or TFIIB) are needed for the binding of…
Explain how HOTAIR plays a role in the transcriptional regulationof particular genes.
a) The best vector to use determine receptor binding protein expression would have been one with a GFP gene (green flourescent protein) attached. State one (1) reason why including this gene would have made the experiment easier and whether you have inserted the receptor binding domain gene before or after the GFP gene.
b) You wish to determine the sucess pf your transformation by detecting the presence of receptor binding domian mRNA. Describe the key steps of the hybrdization technique you would use, clearly stating how you would design the probe to detect your receptor mRNA.
Chapter 14 Solutions
Connect With Learnsmart Labs Online Access For Prescott's Microbiology
Ch. 14.2 - MICRO INQUIRY In what way is on inducer molecule...Ch. 14.2 - MICRO INQUIRY Is allolactose a corepressor or...Ch. 14.2 - Retrieve, Infer, Apply Many genes and operons are...Ch. 14.2 - Prob. 2CCCh. 14.2 - Prob. 3CCCh. 14.3 - MICRO INQUIRY How does this attenuation respond to...Ch. 14.4 - MICRO INQUIRY How does inhibition of translation...Ch. 14.4 - Prob. 1CCCh. 14.4 - Prob. 2CCCh. 14.4 - Prob. 3CC
Ch. 14.4 - Retrieve, Infer, Apply How are translational...Ch. 14.4 - Prob. 5CCCh. 14.5 - MICRO INQUIRY Relative to each promoter, where...Ch. 14.5 - MICRO INQUIRY For what other compounds would you...Ch. 14.5 - Prob. 1CCCh. 14.5 - Retrieve, Infer, Apply What is diauxic growth?...Ch. 14.5 - Retrieve, Infer, Apply Describe the events that...Ch. 14.5 - Retrieve, Infer, Apply E. coli has two phosphate...Ch. 14.5 - Prob. 5CCCh. 14.6 - Prob. 1MICh. 14.6 - MICRO INQUIRY Why does V. harveyi make three...Ch. 14.6 - Prob. 3MICh. 14.6 - Prob. 1CCCh. 14.6 - Prob. 2CCCh. 14.6 - Prob. 3CCCh. 14.6 - Retrieve, Infer, Apply Why might bacteria use...Ch. 14.6 - Prob. 5CCCh. 14.6 - Prob. 6CCCh. 14 - Prob. 1RCCh. 14 - Prob. 2RCCh. 14 - Prob. 3RCCh. 14 - Prob. 4RCCh. 14 - Attenuation affects anabolic pathways, whereas...Ch. 14 - Describe the phenotype of the following E. coli...Ch. 14 - Prob. 3ALCh. 14 - What would be the phenotype of a B. subtilis...Ch. 14 - Propose a mechanism by which a cell might sense...Ch. 14 - Prob. 6ALCh. 14 - Prob. 7ALCh. 14 - Prob. 8ALCh. 14 - Prob. 9AL
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- Please answer fast If the plasmid Lac operon has a mutated lacO operator gene that prevents the repressor from binding, which of the following will occur when lactose is absent? No beta-lactamase will be produced. A functional beta-lactamase will be produced. A non-functional beta-lactamase will be produced. Both a functional and a non-functional beta-lactamase will be produced. If the plasmid Lac operon has a mutated promotor that prevents RNA polymerase from binding, which of the following will occur when lactose is present? No beta-lactamase will be produced. A functional beta-lactamase will be produced. A non-functional beta-lactamase will be produced. Both a functional and a non-functional beta-lactamase will be produced.arrow_forwardAnswer as Directed. Below is the model of a lac operon. lac I lac Z с promoter operator +1 lac Y lac A DNA 1. In the absence of lactose and the presence of glucose in the bacterial growth media, what proteins are bound to the lac control region? Is the operon being transcribed then? 2. In the presence of lactose and the presence of glucose in the bacterial growth media, what proteins are bound to the lac regulatory region? Is the operon being transcribed then? 3. In the presence of lactose and the absence of glucose in the bacterial growth media, what proteins are bound to the lac control region? 4. Why is it adaptive for a bacterium to not express the genes that encode for that lactose utilization proteins when lactose is not available or when glucose is present? 5. Why is it adaptive for the structural genes for using lactose to be under the control of a single promoter, i.e., synthesize a polycistronic message rather than three monocistronic message?arrow_forwardMeasure the uptake of leucine by epithetial cells of the mouse intestine. Measurements of the rate of update of L-leucine, D-Leucine, and L-valine , with and without Na+ in the assay were perform and yield different results (see table below). A) What can you conclude about the properties and mechanism of leucine transporter? B) Would you expect L-leucine uptake to be inhibited by Ouabain, which is a cardiac glycoside drug treatment?arrow_forward
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