ND STONY BROOK UNIVERSITY LOOSELEAF GENETICS: FROM GENES TO GENOMES
6th Edition
ISBN: 9781260406092
Author: HARTWELL, Leland, HOOD, Leroy, Goldberg, Michael
Publisher: Mcgraw-hill Education/stony Brook University
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Chapter 15, Problem 11P
The Saccharomyces cerevisiae nuclear gene ARG8 encodes an enzyme that catalyzes a key step in biosynthesis of the amino acid arginine. This protein is normally synthesized on cytoplasmic ribosomes, but then is transported into mitochondria, where the enzyme conducts its functions. In 1996, T. D. Fox and his colleagues constructed a strain of yeast in which a gene encoding the Arg8 protein was itself moved into mitochondria, where functional protein could be synthesized on mitochondrial ribosomes.
| a. | How could these investigators move the ARG8 gene from the nucleus into the mitochondria, while permitting the synthesis of active enzyme? In what ways would the investigators need to alter the ARG8 gene to allow it to function in the mitochondria instead of in the nucleus? |
| b. | Why might these researchers have wished to move the ARG8 gene into mitochondria in the first place? |
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The Saccharomyces cerevisiae nuclear gene ARG8encodes an enzyme that catalyzes a key step in biosynthesis of the amino acid arginine. This protein isnormally synthesized on cytoplasmic ribosomes, butthen is transported into mitochondria, where the enzyme conducts its functions. In 1996, T. D. Fox andhis colleagues constructed a strain of yeast in which agene encoding the Arg8 protein was itself moved intomitochondria, where functional protein could besynthesized on mitochondrial ribosomes.a. How could these investigators move the ARG8gene from the nucleus into the mitochondria, whilepermitting the synthesis of active enzyme? In whatways would the investigators need to alter theARG8 gene to allow it to function in the mitochondria instead of in the nucleus?b. Why might these researchers have wished to movethe ARG8 gene into mitochondria in the firstplace?
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Chapter 15 Solutions
ND STONY BROOK UNIVERSITY LOOSELEAF GENETICS: FROM GENES TO GENOMES
Ch. 15 - Match each numbered item with the most closely...Ch. 15 - Assuming human cells have on average 1000...Ch. 15 - Reverse translation is a term given to the process...Ch. 15 - The human nuclear genome encodes tRNAs with 32...Ch. 15 - The human mitochondrial genome includes no genes...Ch. 15 - How do you know if the halibut you purchased at...Ch. 15 - Is each of these statements true of chloroplast or...Ch. 15 - Suppose you are characterizing the DNA of a...Ch. 15 - An example of a gene-targeting DNA plasmid vector...Ch. 15 - Which of the following characteristics of...
Ch. 15 - The Saccharomyces cerevisiae nuclear gene ARG8...Ch. 15 - The so-called hypervariable regions HV1 and HV2 of...Ch. 15 - Suppose a new mutation arises in a mitochondrial...Ch. 15 - Describe at least two ways in which the...Ch. 15 - Why are severe mitochondrial or chloroplast gene...Ch. 15 - Suppose you are examining a newly found plant...Ch. 15 - A form of male sterility in corn is inherited...Ch. 15 - Plant breeders have long appreciated the...Ch. 15 - A mutant haploid strain of Saccharomyces...Ch. 15 - Prob. 20PCh. 15 - What characteristics in a human pedigree suggest a...Ch. 15 - The first person in the family represented by the...Ch. 15 - In 1988, neurologists in Australia reported the...Ch. 15 - If you were a genetic counselor and had a patient...Ch. 15 - Kearns-Sayre syndrome KSS, Pearson syndrome, and...Ch. 15 - Many clinically relevant mitochondrial diseases...Ch. 15 - Leigh syndrome is characterized by psychomotor...Ch. 15 - All mutations in mitochondrial genes ultimately...Ch. 15 - How could researchers have determined that the...
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