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MATHEMATICAL The standard free-energy change for the reaction
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Chapter 15 Solutions
Bundle: Biochemistry, Loose-leaf Version, 9th + Owlv2,1 Term Printed Access Card
- MATHEMATICAL For an enzyme that displays MichaelisMenten kinetics, what is the reaction velocity, V (as a percentage of Vmax), observed at the following values? (a) [S]=KM (b) [S]=0.5KM (c) [S]=0.1KM (d) [S]=2KM (e) [S]=10KMarrow_forwardMATHEMATICAL If a reaction can be written AB, and the G is 20kJmol1, what would the substrate/product ratio have to be for the reaction to be thermodynamically favorable?arrow_forwardMATHEMATICAL Consider the reaction AB+C, where G=0.00. (a) What is the value of G (not G) when the initial concentrations of A, B, and C are 1 M, 103M,and106M? (b) Try the same calculations for the reaction D+EF, for the same relative order of concentrations. (c) Try the same calculations for the reaction GH, if the concentrations are 1Mand103M for G and H, respectively.arrow_forward
- MATHEMATICAL Calculate the ATP yield for the complete oxidation of one molecule of palmitic acid (16 carbons). How does this figure differ from that obtained for stearic acid (18 carbons)?arrow_forwardMATHEMATICAL For the Vmax obtained in Question 26, calculate the turnover number (catalytic rate constant) assuming that 131024mol of enzyme were used.arrow_forwardMATHEMATICAL The G for the reaction Citrate Isocitrare is +6.64kJmol1=+1.59kcalmol1. The G for the reaction Isocitrate -Ketoglutrate is 267kJmol1=63.9kcalmol1. What is the G for the conversion of citrate to - ketoglutarate? Is that reaction exergonic or endergonic, and why?arrow_forward
- REFLECT AND APPLY Two biochemistry students are about to use mitochondria isolated from rat liver for an experiment on oxidative phosphorylation. The directions for the experiment specify addition of purified cytochrome c from any source to the reaction mixture. Why is the added cytochrome c needed? Why does the source not have to be the same as that of the mitochondria?arrow_forwardMATHEMATICAL Which is more favorable energetically, the oxidation of succinate to fumarate by NAD+ or by FAD? Give the reason for your answer.arrow_forwardREFLECT AND APPLY In metabolism, glucose-6-phosphate (G6P) can be used for glycogen synthesis or for glycolysis, among other fates. What does it cost, in terms of ATP equivalents, to store G6P as glycogen, rather than to use it for energy in glycolysis? Hint: The branched structure of glycogen leads to 90% of glucose residues being released as glucose-1-phosphate and 10% as glucose.arrow_forward
- REFLECT AND APPLY The enzyme lactate dehydrogenase catalyzes the reaction Pyruvate+NADH+H+lactate+NAD+ NADH absorbs light at 340 nm in the near-ultraviolet region of the electromagnetic spectrum, but NAD1 does not. Suggest an experimental method for following the rate of this reaction, assuming that you have available a spectrophotometer capable of measuring light at this wavelength.arrow_forwardREFLECT AND APPLY The malate-aspartate shuttle yields about 2.5 moles of ATP for each mole of cytosolic NADH. Why does nature use the glycerol-phosphate shuttle, which yields only about 1.5 moles of ATP?arrow_forwardMATHEMATICAL Using the data in Table 15.1, calculate the value of G for the following reaction. Creatinephosphate+GlycerolCreatine+Glycerol-3-phosphate Hint: This reaction proceeds in stages. ATP is formed in the first step, and the phosphate group is transferred from ATP to glycerol in the second step.arrow_forward
- BiochemistryBiochemistryISBN:9781305961135Author:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougalPublisher:Cengage Learning
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