CAMPBELL BIOLOGY IN FOCUS-MOD.MASTERING
3rd Edition
ISBN: 9780135191811
Author: Urry
Publisher: PEARSON
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Chapter 15, Problem 6TYU
Which of the following would not be true of cDNA produced using human brain tissue as the starting material?
- A. It could be amplified by the polymerase chain reaction.
- B. It was produced from pre-mRNA using reverse transcriptase.
- C. It could be labeled and used as a probe to detect genes expressed in the brain.
- D. It lacks the introns of the pre-mRNA.
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Which of these statements is correct regarding the CRISPR/Cas9 system in eukaryotic cells?
A.Single guide RNA (sgRNA) is used to target specific DNA sequences and activate Cas.
B.Bacteriophages are used to inject viral genes into the cell to activated the Cas protein.
C.CRISPR takes advantage of the cell’s DNA polymerase proof-reading activities for DNA replication errors
D.The CRISPR-Cas system can only be used to alter genomes in prokaryotic cells.
E. Cas cuts directly into palindromic restriction sites that it targets in the DNA.
Which of these statements is correct regarding the CRISPR/Cas9 system in eukaryotic cells?
a.Single guide RNA (sgRNA) is used to target specific DNA sequences and activate Cas.
b.Bacteriophages are used to inject viral genes into the cell to activated the Cas protein.
c.CRISPR takes advantage of the cell’s DNA polymerase proof-reading activities for DNA replication errors
d.The CRISPR-Cas system can only be used to alter genomes in prokaryotic cells.
e.Cas cuts directly into palindromic restriction sites that it targets in the DNA.
What are two reasons that a eukaryotic gene (e.g. Green Fluorescent Protein in jellyfish) will not be expressed if it is inserted into a bacterial genome with no alterations?
a. Bacterial genes do not contain introns
b. Bacterial promoters have different consensus sequences than eukaryotic ones
c. Transcription of this gene is affected by the simultaneous processes of transcription and translation
d.
Chapter 15 Solutions
CAMPBELL BIOLOGY IN FOCUS-MOD.MASTERING
Ch. 15.1 - How does binding of the trp corepressor to its...Ch. 15.1 - Describe the binding of RNA polymerase,...Ch. 15.1 - WHAT IF? A certain mutation in E. coli changes the...Ch. 15.2 - Prob. 1CCCh. 15.2 - Compare the roles of general and specific...Ch. 15.2 - Prob. 3CCCh. 15.3 - WHAT IF? Suppose the mRNA being degraded in Figure...Ch. 15.3 - MAKE CONNECTIONS Inactivation of one of the X...Ch. 15.4 - Prob. 1CCCh. 15.4 - WHAT IF? Study the microarray in Figure 15.17. If...
Ch. 15 - If a particular operon encodes enzymes for making...Ch. 15 - The functioning of enhancers is an example of A. a...Ch. 15 - Which of the following is an example of...Ch. 15 - Prob. 4TYUCh. 15 - Prob. 5TYUCh. 15 - Which of the following would not be true of cDNA...Ch. 15 - Prob. 7TYUCh. 15 - SCIENTIFIC INQUIRY Imagine you want to study one...Ch. 15 - FOCUS ON EVOLUTION DNA sequences can act as tape...Ch. 15 - FOCUS ON INTERACTIONS In a short essay (100150...Ch. 15 - Prob. 11TYU
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- Imagine that mutations occurred in one of the inverted repeat sequences within the rho-independent terminator sequence of a bacterium. What would likely be the consequence of this mutation? Select one: a. Transcription may not be initiated at all. b. Transcription may end prematurely. c. Transcription may be delayed. d. The rho protein won't be able to bind and transcription may not be terminate. e. Transcription may not be terminated and result in much longer RNA.arrow_forwardWhich of the following is not true of cDNA produced using human brain tissue as the starting material? (A) It can be amplified by the polymerase chain reaction. (B) It was produced from pre-mRNA using reverse transcriptase. (C) It can be labeled and used as a probe to detect genes expressed in the brain. (D) It lacks the introns of the premRNAarrow_forwardA geneticist induces a mutation in eukaryote cells. The mutation results in an inability to form the poly(A) tail during processing of pre-mRNA. What does this mean for the mature mRNA and what will be the effect on these cells? Possible Answers: A. The mRNA will be spliced, but will not have a 5' cap. B. The mRNA will likely be degraded. C. The mRNA will not be cleaved. D. The mRNA will have too many Gs and Cs.arrow_forward
- E. coli are grown on a medium containing lactose. Once glucose is added to the medium, the bacteria stop fermenting lactose. Which of the following BEST explains the observed effect? A. Cellular levels of cAMP are lowB.Glucose is bound to the promoter C. Repressor protein is bound to the operator D. Repressor protein is bound to the promoterarrow_forwardDavid Pribnow studied the base sequences of promoters in bacteria and bacterial viruses. He found two conserved regions in these promoters (the -10 box and the -35 box). These two regions of the promoter Select one: a. signal the initiation site b. bind the sigma subunit that is associated with RNA polymerase O c. attach the correct nucleotide triphosphate to the template DNA strand d. separate the two DNA strandsarrow_forwardWhich of the following statements about CRISPR-Cas9 is correct? O A. It relies on the natural process of DNA repair. B. Regardless of the DNA target, the sequence of the guide RNA is always the same. C. Cas9 is only involved in recruitment of guide RNA to its target DNA. D. It is not precise enough to make a single base pair edit.arrow_forward
- Which of the following plays a major part in determining whether or not RNA polymerase will transcribe a parti sequence? Select all that apply. A. A ribosome is already bonded to the gene sequence B. A complete set of transcription factors bind to DNA associated with that gene C. The chromatin structure is loosened in the region of that gene D. RNA polymerase binds to the promoter of that gene ..arrow_forwardWhich of the following statements is/are TRUE for transcription? A. RNA polymerase uses one strand of DNA, the antisense strand, for RNA synthesis. B. The first ribonucleotide binds to the Pribnow region. C. RNA polymerase moves from 5’ → 3’, while the RNA polymerization is from 3’ → 5’. D. All four ribonucleoside monophosphates are required.arrow_forwardArrange the statements in their proper order by writing the corresponding letter (e.g. A) for each statement in the space provided below. A. The single-stranded RNA would complement the target RNA. B. Gene expression is inactivated once the mRNA is no longer accessible for translation. C. The risk-induced silencing complex which is composed of RNA and protein subunits is formed. D. Double-stranded, non-coding RNA is cleaved by Dicer. E. The mRNA can be cleaved or remain bound by the RISC. 1. 2. 3. 4. 5.arrow_forward
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