Mindtap Biology, 1 Term (6 Months) Printed Access Card For Starr/taggart/evers/starr's Biology: The Unity And Diversity Of Life
15th Edition
ISBN: 9781337408493
Author: STARR, Cecie; Taggart, Ralph; Evers, Christine; Starr, Lisa
Publisher: Cengage Learning
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Chapter 15, Problem 9SQ
Summary Introduction
Introduction: DNA cloning refers to a combination of laboratory techniques that are utilized to produce specific DNA sequences at a large scale inside living cells.
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Put the following tasks in the order they would occur during a DNA cloning experiment. a. using DNA ligase to seal DNA fragments into vectors b. using a probe to identify a clone in the library c. sequencing the DNA of the clone d. making a DNA library of clones e. cutting genomic DNA with restriction enzymes
Arrange the following steps in the sequence they would happen in a DNA cloning experiment.
a. sealing DNA fragments into vectors with DNA ligase;
b. utilizing a probe to detect a clone in the library;
c. sequencing the clone's DNA;
d. creating a DNA library of clones;
e. cutting genomic DNA with restriction enzymes.
A.
e,a,d,b,c
B.
a,d,b,c,e
C.
c,b,e,a,d
D.
e,d,a,c,b
What is a cloning vector?
A.
The DNA probe used to locate a particular gene in the genome.
B.
An agent such as plasmid, used to transfer DNA from an in vitro solution into a living cell.
C.
The laboratory apparatus used to clone genes.
D.
An enzyme that cuts DNA into restriction fragments.
Chapter 15 Solutions
Mindtap Biology, 1 Term (6 Months) Printed Access Card For Starr/taggart/evers/starr's Biology: The Unity And Diversity Of Life
Ch. 15 - Enhanced Spatial Learning Ability in Mice...Ch. 15 - Enhanced Spatial Learning Ability in Mice...Ch. 15 - Prob. 3DAACh. 15 - Prob. 4DAACh. 15 - _________ cut(s) DNA molecules at specific sites....Ch. 15 - Prob. 2SQCh. 15 - Prob. 3SQCh. 15 - For each species, all ________ in the complete set...Ch. 15 - A set of cells that host various DNA fragments...Ch. 15 - Prob. 6SQ
Ch. 15 - Prob. 7SQCh. 15 - Prob. 8SQCh. 15 - Prob. 9SQCh. 15 - Prob. 10SQCh. 15 - A transgenic organism _______. a. carries a gene...Ch. 15 - True or false? Some transgenic organisms can pass...Ch. 15 - Taq polymerase is used for PCR because it _______....Ch. 15 - _____ can correct a genetic defect in an...Ch. 15 - Match each term with the most suitable...Ch. 15 - Match the method with the appropriate enzyme....Ch. 15 - Prob. 1CTCh. 15 - Restriction enzymes in bacterial cytoplasm cut...Ch. 15 - The results of a paternity test using short tandem...
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- Cloning Genes Is a Multistep Process In cloning human DNA, why is it necessary to insert the DNA into a vector such as a bacterial plasmid?arrow_forwardFollowing are four processes common to most cloning experiments: a) transforming bacteria b) plating bacteria on selective medium c) cutting DNA with restriction endonucleases d) ligating DNA fragments Place components of this list in the order in which they would most likely occur during a cloning experiment.arrow_forwardThe following question is related to Restriction Enzymes and RFLP. Using EcoRl, how many cuts were performed on DNA sequence A? A.) 2 B.) 3 C.) 4arrow_forward
- Please answer these two questions regarding PCR: a) Why do you need to perform PCR on DNA obtained from a crime scene? b) Why so forensic labs analyze non-coding DNA rather than genes?arrow_forwardWhen we discuss PCR and other similar techniques, the term Tm is often used. This refers to a.The temperature where the DNA molecules denature. b.The temperature of the first step in a PCR cycle. c.The temperature where half of the DNA molecules are denatured. d.Tm is the same as the annealing temperature. e.The Temperature at which all of the DNA molecules are dentaturedarrow_forwardEnzymes used to cut genes in recombinant DNA research are known as ____. a. DNA polymerases b. transcriptases c. replicases d. ligases e. restriction enzymesarrow_forward
- Which of the following is necessary for a PCR reaction to proceed? a) the sequence of the ends of the DNA to be amplified must be known. b) the sequence of restriction endonuclease recognition sites in the DNA to be amplified and in the plasmid, where the amplified DNA fragment will be cloned must be known. c) The complete sequence of the DNA to be amplified must be known. d) The sequence of restriction endonuclease recognition sites in the DNA to be amplified must be knownarrow_forwardFrom your knowledge about DNA microarray, answer the following: Why RT-PCR is important in the sample preparation to perform expression microarray experiment?(arrow_forwardWhich technique rapidly replicates specific DNA fragments without cloning in cells? (a) gel electrophoresis (b) cDNA libraries (c) DNA probe (d) restriction fragment length polymorphism (e) polymerase chain reactionarrow_forward
- Regarding the PCR technique, what is false?a. It can produce multiple copies of DNA.b. It is the same as DNA fingerprinting.c. It is not a time-consuming process.d. It cannot successfully copy whole genesarrow_forwardYou have extracted a long piece of DNA from a human cell and you want to extract the gene of interest for you to clone it. Assuming that you know the sequence of the DNA what methods can youuse to amplify the gene of interest? A. antibody purification B. polymerase chain reaction C. none of the above D. ligationarrow_forwardIn next-generation sequencing, which of these advances allows for massively parallel sequencing? a. Pieces of DNA are fixed to a surface, so we can tell which new nucleotides were added to each piece. b. DNA sequences are read in real-time as nucleotides are added to each piece. c. Each segment of the genome can be pieced back together through shotgun alignment d. Single molecules of DNA can be read without the need for amplification.arrow_forward
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