Biochemistry (Looseleaf)
9th Edition
ISBN: 9781319114800
Author: BERG
Publisher: MAC HIGHER
expand_more
expand_more
format_list_bulleted
Concept explainers
Question
Chapter 16, Problem 31P
Interpretation Introduction
Interpretation:
The radioisotipe distribution of 2,3-BPG which is formed by addition of BPG muatse needs to be determined.
Concept introduction:
BPG (bisphosphoglycerate mutase) enzyme is found in placenta and red blood cells (RBCs). It converts 1,3-bisphoshpglycerate to 2,3-bis phosphoglycerate. The formation of 2,3-BPG is inefficient. It is basically required to shift the equilibrium of hemoglobin towards the deoxy state.
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
47. No exchange. Mice that completely lack ATP-ADP translocase (ANT- /ANT - ) can be made by using the knockout technique. Remarkably, these mice are viable but have the following pathological conditions: (1) high serum levels of lactate, alanine, and succinate; (2) little electron transport; and (3) a six- to eightfold increase in the level of mitochondrial H2O2 compared with that in normal mice. Provide a possible biochemical explanation for each of these conditions.
Thin Layer chromatography (TLC).
Explain the biochemical principle behind the separation of carbohydrates molecules by TLC as performed in practical 5. Explain which properties molecules must have to travel a short and large distance, respectively.
Ketohexose sugars can form 8 different stereoisomers. How many of those isomers can be distinguished and resolved by TLC as performed in the practical and why?
no more than 100 words total
Need help
Sesamin is one of the major lignans found in sesame oil. Sesamin-metabolic pathways remain uncharacterized at both the enzyme and gene levels. However, one microorganism showed significant sesamin-degrading activity and was identified as Sinomonas sp. no. 22. A sesamin- metabolizing enzyme named SesA was purified from this strain and characterized. Explain how sesamin methylene transferase was identified and characterized and explain the mechanism. Based on your knowledge, what other assays could be run to characterize this enzyme?
Chapter 16 Solutions
Biochemistry (Looseleaf)
Ch. 16 - Prob. 1PCh. 16 - Prob. 2PCh. 16 - Prob. 3PCh. 16 - Prob. 4PCh. 16 - Prob. 5PCh. 16 - Prob. 6PCh. 16 - Prob. 7PCh. 16 - Prob. 8PCh. 16 - Prob. 9PCh. 16 - Prob. 10P
Ch. 16 - Prob. 11PCh. 16 - Prob. 12PCh. 16 - Prob. 13PCh. 16 - Prob. 14PCh. 16 - Prob. 15PCh. 16 - Prob. 16PCh. 16 - Prob. 17PCh. 16 - Prob. 18PCh. 16 - Prob. 19PCh. 16 - Prob. 20PCh. 16 - Prob. 21PCh. 16 - Prob. 22PCh. 16 - Prob. 23PCh. 16 - Prob. 24PCh. 16 - Prob. 25PCh. 16 - Prob. 26PCh. 16 - Prob. 27PCh. 16 - Prob. 28PCh. 16 - Prob. 29PCh. 16 - Prob. 30PCh. 16 - Prob. 31PCh. 16 - Prob. 32PCh. 16 - Prob. 33PCh. 16 - Prob. 34PCh. 16 - Prob. 35PCh. 16 - Prob. 36PCh. 16 - Prob. 37PCh. 16 - Prob. 38PCh. 16 - Prob. 39PCh. 16 - Prob. 40PCh. 16 - Prob. 41PCh. 16 - Prob. 42PCh. 16 - Prob. 43PCh. 16 - Prob. 44PCh. 16 - Prob. 45PCh. 16 - Prob. 46PCh. 16 - Prob. 47PCh. 16 - Prob. 48PCh. 16 - Prob. 49PCh. 16 - Prob. 50PCh. 16 - Prob. 51PCh. 16 - Prob. 52PCh. 16 - Prob. 53PCh. 16 - Prob. 54PCh. 16 - Prob. 55PCh. 16 - Prob. 56PCh. 16 - Prob. 57PCh. 16 - Prob. 58PCh. 16 - Prob. 59PCh. 16 - Prob. 60PCh. 16 - Prob. 61P
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biochemistry and related others by exploring similar questions and additional content below.Similar questions
- The nitrogenase complex can reduce molecules other than N2. Provide the structures for the products for each of the following substrates (real and hypothetical) that contain triple bonds: hydrogen cyanide, dinitrogen, and acetylene.arrow_forwardLysozyme’s use of aspartic acid and glutamic acid, to break the glycosidic bonds of the eubacterial cell wall (composed of peptidoglycan), illustrates the common enzymatic pattern of: hydrolysis (catabolic), using nonpolar amino acids at the active site hydrolysis (catabolic), using polar amino acids at the active site condensation (anabolic), using nonpolar amino acids at the active site condensation (anabolic), using polar amino acids at the active site all of the abovearrow_forwardSubstituent effects. What is the pHPpH of a 0.1 M0.1 M solution of chloroacetic acid (CICH2 COOH, pKa=2.86)? (CICH, COOH, pK, = 2.86) ? doarrow_forward
- Growth of yeast cells In a controlled laboratory experiment, yeast cells are grown in an automated cell culture system that counts the number P of cells present at hourly intervals. The num- ber after t hours is shown in the accompanying figure. 250 200 150 100 50 0123456 7 a. Explain what is meant by the derivative P'(5). What are its units? b. Which is larger, P'(2) or P'(3)? Give a reason for your answer.arrow_forwardPlz answer correctly do not copy. Question- Arsinate binds to reduced thio groups such as those found in cystiene residues in proteins, lipoate or glutathione. The resulting inhibition leads to central nervous system pathologies. The binding of arsinate to the dihydrolipoyl groups inhibits which of the following enzyme(s)? succinate dehydrogenase malate dehydrogenase pyruvate dehydrogenase complex branched-chain amino acid dehydrogenase complex isocitrate dehydrogenase alpha-ketoglutarate dehydrogenase complexarrow_forwardSerine protease enzyme mutation To show differences in the effect of the nucleophilic attack of the carbonyl group (C=O) of peptide bond between the catalytic triad of serine, histidine and aspartic acid, and another catalytic triad contains alanine, histidine and aspartic acid Provide/ draw an example of catalytic mechanism with catalytic triad contains alanine, histidine and aspartic Please answer completely will give rating surelyarrow_forward
- NH3 i, CH₂ HN 'S Co A NH CH₂ CH₂ NH 1. Describe the role of Cys in the catalytic mechanism shown. 2. Describe/predict the microenvironment that could allow for Cys to be ionized in the catalytic mechanism.arrow_forwardMolecular detail of spike Y453Farrow_forwardJust Arrange. The enzyme serine hydroxymethyltransferase (SHMT) requires pyridoxal phosphate (PLP) as a cofactor. Arrange the steps of the likely mechanism for SHMT‑catalyzed serine degradation (producing glycine and N5,N10‑methylenetetrahydrofolate)arrow_forward
- Help please. This question is specifically asking for the identification of the biomolecules that are attached to the sphingosine core, then we need to answer what bond causes those biomolecules to be connected to the sphingosine, what reaction created that bond (maybe addition or oxidation etc.), what were the starting materials and lastly what reagents or conditions are needed for the reaction to occur. Thank you!arrow_forwardNH3 gala. S Co A C H₂ R-C S CH₂ HN ĐÌNH ΝΗ CH₂ CH₂ NH 3. Compare and contrast how thiolase and chymotrypsin creates and stabilizes its intermediates.arrow_forwardRefer to the reaction scheme of the GAPDH catalyzed formation of 1,3-BPG. In the reaction, the nucleophile in step (4) is an inorganic phosphate molecule. The enzyme active site is elaborately constructed so that inorganic phosphate, not water, acts as the nucleophile. What advantage is gained by using inorganic phosphate, instead of water, as the nucleophile? Discuss in terms of the energy yield of glycolysis. No need to calculate the total net energy yield of glycolysis.arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
BiochemistryBiochemistryISBN:9781305577206Author:Reginald H. Garrett, Charles M. GrishamPublisher:Cengage Learning
Biochemistry
Biochemistry
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Cengage Learning