EBK CONCEPTS OF GENETICS
12th Edition
ISBN: 9780134818979
Author: Killian
Publisher: YUZU
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Chapter 16, Problem 8PDQ
Summary Introduction
To determine: The reasons due which the lac repressor was isolated.
Introduction: Lac repressor inhibits the genes that are responsible for lactose
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What form of lac repressor mutation might be both constitutive and trans-dominant?
What is the probability that the palindromic symmetry of the trp repressor target DNA sequence is merely accidental?
Suppose that E. coli sustains a mutation in its gene for the lac operon repressor such that the repressor is ineffective. What effect would this have on the bacterium’s ability to catabolize lactose? Would the mutant strain have an advantage over wild-type cells? Explain your answer.
Chapter 16 Solutions
EBK CONCEPTS OF GENETICS
Ch. 16 - Even though the lac Z, Y, and A structural genes...Ch. 16 - Predict the level of genetic activity of the lac...Ch. 16 - Prob. 1CSCh. 16 - Prob. 2CSCh. 16 - Prob. 3CSCh. 16 - HOW DO WE KNOW? In this chapter, we focused on the...Ch. 16 - Prob. 2PDQCh. 16 - Contrast positive versus negative control of gene...Ch. 16 - Contrast the role of the repressor in an inducible...Ch. 16 - For the lac genotypes shown in the following...
Ch. 16 - For the genotypes and conditions (lactose present...Ch. 16 - The locations of numerous lacI and lacIS mutations...Ch. 16 - Prob. 8PDQCh. 16 - Prob. 9PDQCh. 16 - Predict the effect on the inducibility of the lac...Ch. 16 - Erythritol, a natural sugar abundant in fruits and...Ch. 16 - Prob. 12PDQCh. 16 - Prob. 13PDQCh. 16 - Neelaredoxin is a 15-kDa protein that is a gene...Ch. 16 - The creation of milk products such as cheeses and...Ch. 16 - Both attenuation of the trp operon in E. coli and...Ch. 16 - Prob. 17PDQCh. 16 - Prob. 18ESPCh. 16 - In a theoretical operon, genes A, B, C, and D...Ch. 16 - A bacterial operon is responsible for the...Ch. 16 - A marine bacterium is isolated and shown to...Ch. 16 - Prob. 22ESPCh. 16 - Prob. 23ESP
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- What would happen if the operator sequence of the trp operon contained a mutation that prevented the repressor protein from binding to the operator? (Explain what would happen in both the presence and absence of tryptophan)arrow_forwardIn the bacterium,Martian coli, it was discovered that the lac operon is positively regulated. Lactose, when present, binds to the regulatory protein A. The protein A-lactose complex then binds to the operator domain 0 and induces synthesis of lacZ+ and lacY+ MRNAS. a) What would be the most common phenotype of o- mutants in terms of lactose utilization? Explain your answer. b) What would be the most common phenotype of A- mutants? Explain your answer.arrow_forwardDescribe what would happen to the lac operon in a low-lactose environment and in a high lactose environment. a) How will each of these environments affect gene expression? b) What would happen to the repressor in each case? c) How will each of these environments affect RNA polymerase?arrow_forward
- What is the function of the repressor encoded by the lacI gene?arrow_forwardExplain the process of attenuation in the trp operon under the following conditions: (i) No tryptophan (ii) High tryptophan levelsarrow_forwardWhat experimental results would indicate that the mutation lacISlacIS is dominant to lacI+lacI+? In lacISlacIS/lacI+lacI+ partial diploids, the lac operon is in a repressed state in the absence of lactose. In lacISlacIS/lacI+lacI+ partial diploids, the lac operon is in a constitutive state in the absence of the repressor. In lacISlacIS/lacI+lacI+ partial diploids, the lac operon is in an activated state in the presence of lactose. In lacISlacIS/lacI+lacI+ partial diploids, the lac operon is in a repressed state in the absence of the repressor. In lacISlacIS/lacI+lacI+ partial diploids, the lac operon is in a repressed state in the presence of lactose.arrow_forward
- Suppose that E. coli sustains a mutation in its gene for the lac operon repressor making the repressor ineffective . How would this mutation affect the bacterium's ability to catabolize lactose? Would the mutant strain have an advantage over the wild-type strain? Explain your answer.arrow_forwardIn the absence of lactose and the lacI repressor gene is mutated such that the repressor protein product can no longer bind to the operator, the structural genes will: [ Select ] ["No longer be expressed", "Be constantly expressed", "Be deleted from the operon"] In the presence of lactose and the lacI repressor gene is mutated such that the repressor protein product can no longer bind to the operator, the structural genes will: [ Select ] ["No longer be expressed", "Be constantly expressed", "Be deleted from the operon"]arrow_forwardwhat is the nature and likely location(s) of a mutant that would, 1)allow constitutive expression of the lac gene? 2)prevent the cell from responding to lactose ( genes are not induced when exposed to lactose)? 3) not allow the cell to utilize lactose even when the genes are inducedarrow_forward
- What would happen if the operator sequence of the lac operon contained a mutation that prevented the repressor protein from binding the operator? (Explain what would happen both in the presence and absence of lactose)arrow_forwardExplain how elements of the lac operon are exploited to regulate recombinant protein production in E. coli.arrow_forwardSuppose that a new mutation lacIes, ('es' stands for ‘extra-strength’) has been discovered in the lac operon that allows the lac-repressor to bind even to the lacOc operator. Other properties of lacIes repressor remain normal (that is the same as of lacI+). Given this genotype Is P+ Oc Z+ Y- / Ies P+ O+ Z+ Y+ , under which conditions will the gene lacZ be transcribed to produce β-galactosidase and the gene lacY transcribed to produce permease? A) With lactose, β-galactosidase and permease are produced, without lactose they are not produced B) With lactose, β-galactosidase is produced, without lactose it is not; permease is never produced C) β-galactosidase is always produced and permease is never produced D) With lactose, β-galactosidase and permease are produced, without lactose they are also produced E) β-galactosidase is always produced and permease is produced only in the presence of lactosearrow_forward
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