PRESCOTTS GENERAL MICROBIOLOGY
11th Edition
ISBN: 9781307412420
Author: WILLEY
Publisher: MCG/CREATE
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Textbook Question
Chapter 16.3, Problem 4CC
Explain how the following DNA alterations and replication errors would be corrected (there may be more than one way): base addition errors by DNA polymerase III during replication, thymine dimers, AP sites, methylated guanines, and gaps produced during replication.
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Chapter 16 Solutions
PRESCOTTS GENERAL MICROBIOLOGY
Ch. 16.1 - Retrieve, Infer, Apply List three ways in which...Ch. 16.1 - Compare and contrast the means by which the...Ch. 16.1 - Give examples of intragenic and extragenic...Ch. 16.1 - Retrieve, Infer, Apply Sometimes a point mutation...Ch. 16.1 - Retrieve, Infer, Apply Why might a missense...Ch. 16.2 - How would you screen for a tryptophan auxotroph?...Ch. 16.2 - Why is a small amount of histidine added to the...Ch. 16.2 - Retrieve, Infer, Apply Describe how replica...Ch. 16.2 - Retrieve, Infer, Apply Why are mutant selection...Ch. 16.2 - Retrieve, Infer, Apply Briefly discuss how...
Ch. 16.2 - Describe how you would isolate a mutant that...Ch. 16.2 - Prob. 5CCCh. 16.3 - How is mismatch repair similar to DNA polymerase...Ch. 16.3 - How is damaged DNA recognized by the UvrAB...Ch. 16.3 - Prob. 1CCCh. 16.3 - Retrieve, Infer, Apply What role does DNA...Ch. 16.3 - Retrieve, Infer, Apply When E. coli cells are...Ch. 16.3 - Explain how the following DNA alterations and...Ch. 16.4 - An antibiotic-resistance gene located on a...Ch. 16.4 - What four fates can DNA have after entering a...Ch. 16.4 - How does homologous recombination differ from...Ch. 16.5 - What features are common to all types of...Ch. 16.5 - How does a transposon differ from an insertion...Ch. 16.5 - What is simple (cut-and-paste) transposition? What...Ch. 16.5 - What effect would you expect the existence of...Ch. 16.6 - Prob. 1MICh. 16.6 - What is bacterial conjugation and how was it...Ch. 16.6 - For F+, Hfr, and F strains of E. coli, indicate...Ch. 16.6 - Describe how F+ F and Hfr conjugation processes...Ch. 16.6 - Compare and contract F+ F and F F conjugation.Ch. 16.7 - According to this model, what would happen if DNA...Ch. 16.7 - Prob. 1CCCh. 16.7 - Describe how transformation occurs in S....Ch. 16.7 - Discuss two ways in which artificial...Ch. 16.8 - Compare the number of transducing particles that...Ch. 16.8 - Why cant the gal and bio genes be transduced by...Ch. 16.8 - Describe generalized transduction and how it...Ch. 16.8 - What is specialized transduction and how does it...Ch. 16.8 - How might one tell whether horizontal gene...Ch. 16.8 - Why doesnt a cell lyse after successful...Ch. 16.8 - Describe how conjugation, transformation, and...Ch. 16.9 - As a replicative transposon, what would happen if...Ch. 16 - Prob. 1RCCh. 16 - Prob. 2RCCh. 16 - Prob. 3RCCh. 16 - Prob. 4RCCh. 16 - Prob. 5RCCh. 16 - Prob. 6RCCh. 16 - Mutations are often considered harmful. Give an...Ch. 16 - Mistakes made during transcription affect the cell...Ch. 16 - Suppose that transduction took place when a U-tube...Ch. 16 - Suppose that you carried out a U-tube experiment...Ch. 16 - Prob. 5ALCh. 16 - Prob. 6ALCh. 16 - Prob. 7AL
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- The type of DNA replication error illustrated in the diagram below is _______________________.arrow_forwardGive 3 strand slippage and trinucleotide repeat disorder examplesarrow_forwardMatch the following phenotypes with the mutation that would be the most likely to be its cause. The mutant proteins are listed below, followed by the protein domain that is mutated in each case, followed by the amino acid change that is a result of the mutation. __ DNA replication in E.coli stops after one replication cycle, and the new DNA strands contain a large number of individual RNA nucleotides spaced throughout the newly synthesized strand. __ An E.coli mutant shows normal replication rates early I DNA replication, but the stops replicating DNA and is unable to continue. __ A mutant human cell line is unable to identify mutations occurring at times when the DNA is not being replicated. __ An E.coli mutant shows high mutation rates after DNA replication, and you observe fragments of methylated DNA cut out from the newly-synthesized chromosome.arrow_forward
- explain strand-slippage replication for STR mutationarrow_forwardExplain the connection between defects in DNA repair systemsand the inherited human disease xeroderma pigmentosum.arrow_forwardThe letters ABCDEFGH represent a normal DNA sequence. Indicate the type of mutation present in each of the following situations: a) ABCCDEFGH b) ABCDEGHarrow_forward
- 1.1 What components must be available for DNA polymerase III to proceed with DNA synthesis during replication? 1.2 What will the consequences for the cell be if the following enzymes are not functioning? DNA polymerase III Primase DNA ligasearrow_forwardExplain why base excision repair, nucleotide excision repair, and mismatch repair—which all require nucleases to excise damaged DNA—require DNA ligase.arrow_forwardWhich of the following statements regarding Nucleotide Excision Repair (NER) and Base Excision Repair (BER) is true? Only NER involves the action of DNA ligase to seal nicks in the DNA backbone. Both NER and BER involve DNA strand cleavage by an endonuclease. Both NER and BER can be activated by exposure to visible light. Only BER requires DNA polymerase. Both NER and BER involve the creation of an apyrimidinic (AP) site.arrow_forward
- Discuss about conservative replication ?arrow_forwardList three ways in which an unrepaired double-stranded DNA break can be highly dangerous to the cell in which it occurs.arrow_forwardWhich of the following is NOT true regarding E. coli replication on the lagging strand? initially synthesized as Okazaki fragments primers are removed by DNA polymerase I. synthesized discontinuously. performed in a 5'→3' direction of synthesisarrow_forward
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