PRESCOTTS GENERAL MICROBIOLOGY
11th Edition
ISBN: 9781307412420
Author: WILLEY
Publisher: MCG/CREATE
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Textbook Question
Chapter 16.1, Problem 2CC
Compare and contrast the means by which the mutagens 5-bromouracil, methyl-nitrosoguanidine, proflavin, and UV radiation induce mutations.
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Why is it practical to test carcinogens by a mutagenesis assay?
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Fill the Table with mutagenic agents and provide their type (physical, chemical, biological) and their classification based on their effect (teratogenic, carcinogenic, clastogenic, or non-specific), together with their modes of action.
Mutagen
Type of Mutagen
Classification based on effect
Action
Cobalt
Nickel
Methylhydrazine
Temozolomide
Ethyl ethane sulfate
Chapter 16 Solutions
PRESCOTTS GENERAL MICROBIOLOGY
Ch. 16.1 - Retrieve, Infer, Apply List three ways in which...Ch. 16.1 - Compare and contrast the means by which the...Ch. 16.1 - Give examples of intragenic and extragenic...Ch. 16.1 - Retrieve, Infer, Apply Sometimes a point mutation...Ch. 16.1 - Retrieve, Infer, Apply Why might a missense...Ch. 16.2 - How would you screen for a tryptophan auxotroph?...Ch. 16.2 - Why is a small amount of histidine added to the...Ch. 16.2 - Retrieve, Infer, Apply Describe how replica...Ch. 16.2 - Retrieve, Infer, Apply Why are mutant selection...Ch. 16.2 - Retrieve, Infer, Apply Briefly discuss how...
Ch. 16.2 - Describe how you would isolate a mutant that...Ch. 16.2 - Prob. 5CCCh. 16.3 - How is mismatch repair similar to DNA polymerase...Ch. 16.3 - How is damaged DNA recognized by the UvrAB...Ch. 16.3 - Prob. 1CCCh. 16.3 - Retrieve, Infer, Apply What role does DNA...Ch. 16.3 - Retrieve, Infer, Apply When E. coli cells are...Ch. 16.3 - Explain how the following DNA alterations and...Ch. 16.4 - An antibiotic-resistance gene located on a...Ch. 16.4 - What four fates can DNA have after entering a...Ch. 16.4 - How does homologous recombination differ from...Ch. 16.5 - What features are common to all types of...Ch. 16.5 - How does a transposon differ from an insertion...Ch. 16.5 - What is simple (cut-and-paste) transposition? What...Ch. 16.5 - What effect would you expect the existence of...Ch. 16.6 - Prob. 1MICh. 16.6 - What is bacterial conjugation and how was it...Ch. 16.6 - For F+, Hfr, and F strains of E. coli, indicate...Ch. 16.6 - Describe how F+ F and Hfr conjugation processes...Ch. 16.6 - Compare and contract F+ F and F F conjugation.Ch. 16.7 - According to this model, what would happen if DNA...Ch. 16.7 - Prob. 1CCCh. 16.7 - Describe how transformation occurs in S....Ch. 16.7 - Discuss two ways in which artificial...Ch. 16.8 - Compare the number of transducing particles that...Ch. 16.8 - Why cant the gal and bio genes be transduced by...Ch. 16.8 - Describe generalized transduction and how it...Ch. 16.8 - What is specialized transduction and how does it...Ch. 16.8 - How might one tell whether horizontal gene...Ch. 16.8 - Why doesnt a cell lyse after successful...Ch. 16.8 - Describe how conjugation, transformation, and...Ch. 16.9 - As a replicative transposon, what would happen if...Ch. 16 - Prob. 1RCCh. 16 - Prob. 2RCCh. 16 - Prob. 3RCCh. 16 - Prob. 4RCCh. 16 - Prob. 5RCCh. 16 - Prob. 6RCCh. 16 - Mutations are often considered harmful. Give an...Ch. 16 - Mistakes made during transcription affect the cell...Ch. 16 - Suppose that transduction took place when a U-tube...Ch. 16 - Suppose that you carried out a U-tube experiment...Ch. 16 - Prob. 5ALCh. 16 - Prob. 6ALCh. 16 - Prob. 7AL
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- And to follow up previous question, how do you exactly identify the mutations in driving gene, as I need to give evidence.arrow_forwardContrast the various types of DNA repair mechanisms known to counteract the effects of UV radiation. What is the role of visible light in repairing UV-induced mutations?arrow_forwardWhich of the following types of physical mutagens produces thymine dimmer mutations? A- gamma rays B- X-rays C- ionizing radiation D- ultraviolet light E- microwavearrow_forward
- Discuss the times in a person’s life when it is most important toavoid mutagens. Which parts of a person’s body should be the mosthighly protected from mutagens?arrow_forwardFill the Table with mutagenic agents and provide their type (physical, chemical, biological) and their classification based on their effect (teratogenic, carcinogenic, clastogenic, or non-specific), together with their modes of action. Mutagen Type of Mutagen Classification based on effect Action UV – radiation X-Ray radiation Virus Heat 5-bromouracil Ethidium bromide 2-aminopurine Acridine orange Proflavine Cobalt Nickel Methylhydrazine Temozolomide Ethyl ethane sulfatearrow_forwardFill the Table with mutagenic agents and provide their type (physical, chemical, biological) and their classification based on their effect (teratogenic, carcinogenic, clastogenic, or non-specific), together with their modes of action. Mutagen Type of Mutagen Classification based on effect Action 2-aminopurine Acridine orange Proflavinearrow_forward
- Compare and contrast the action of DNA damaging agents that lead to: 1. Point mutations . 2. Frameshift mutations 3. Formation of DNA dimersarrow_forwardWhat type of mutations is obtained for the following mutagens. Nitrous acid. Point, addition, or deletion. 5-bromouracil. Point, addition, or deletion. Acridine. Point, addition, or deletion.arrow_forwardExplain the Detection of the sickle-cell mutation by sequencing of PCR products?arrow_forward
- List three ways in which spontaneous mutations might arise.arrow_forwardFill the Table with mutagenic agents and provide their type (physical, chemical, biological) and their classification based on their effect (teratogenic, carcinogenic, clastogenic, or non-specific), together with their modes of action. Mutagen Type of Mutagen Classification based on effect Action Heat 5-bromouracil Ethidium bromidearrow_forwardExplain with help of diagrams the mutagenic effects on DNA of following mutagens? Below is an example i. Ultraviolet light ii. X- raysiii. Base analogsiv. Hydroxylaminev. nitrous acidvi. ethylmethane sulfonatearrow_forward
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