BIOLOGY 12TH ED (LL) W/ CONNECT
12th Edition
ISBN: 9781264898091
Author: Raven
Publisher: MCG
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Chapter 17, Problem 1S
Many human proteins, such as hemoglobin, are only functional as an assembly of multiple subunits. Assembly of these functional units occurs within the endoplasmic reticulum and Golgi apparatus of a eukaryotic cell. Discuss what limitations, if any, exist to the large-scale production of genetically engineered hemoglobin.
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Bacteria are often the preferred hosts for genetic engineering projects by splicing in novel genes from eukaryotes into plasmids, which are moved into competent bacteria. For instance, the gene for human insulin was isolated and moved into a bacterium, which can now produce the much-needed chemical. Previously, type 1 diabetics had to rely on professionals that gathered insulin from human cadavers, cows, and pigs. In order for this feat of genetic engineering to occur, researchers had to start with an unspliced mRNA transcript for h insulin.
Agree/Disagree? Explain your response.
Before this experiment, Dr Merrick didn’t know whether the OBP that he cloned was a type of myoglobin or hemoglobin from mammoth. From his data, he is now quite sure that OBP cannot be myoglobin from mammoth. How does he know? Explain your reasoning.
Termicin is a small antifungal protein in termites that is produced by cells and secreted into termite saliva in response to a pathogen. In vitro translation of
the termicin-encoding gene is performed, and the effects of that product are compared to those of termicin extracted from a termite. You see that extracted
termicin exhibits more antifungal behavior than in vitro translated termicin. After further analysis, you see that extracted termicin contains 3 disulfide bonds,
while in vitro translated termicin contains zero. The addition of microsomes to the in vitro translation reaction results in termicin with all 3 disulfide
bonds. What experimental condition is most likely responsible for this difference?
A. in vitro translation was not performed at the correct temperature affecting protein folding
B. a mutation occurred during in vitro translation, leading to differences in disulfide bond formation
O C. the UPR can not be activated in vitro, therefore, this protein can only be…
Chapter 17 Solutions
BIOLOGY 12TH ED (LL) W/ CONNECT
Ch. 17.1 - Prob. 1LOCh. 17.1 - Prob. 2LOCh. 17.1 - Describe the construction and uses of recombinant...Ch. 17.2 - Relate the process of DNA replication to PCR.Ch. 17.2 - Compare and contrast PCR, RT-PCR, and quantitative...Ch. 17.3 - Prob. 1LOCh. 17.3 - Prob. 2LOCh. 17.3 - Describe the pros and cons of RNA interference and...Ch. 17.4 - Explain how the universal nature of the genetic...Ch. 17.4 - Compare and contrast knockout, knockin, and...
Ch. 17.4 - Prob. 3LOCh. 17.5 - Describe the benefits of biofuel production from...Ch. 17.5 - Prob. 2LOCh. 17.5 - Prob. 3LOCh. 17.6 - Prob. 1LOCh. 17.6 - Compare and contrast FISH and gene chip...Ch. 17.6 - Describe how immunoassays can be used to diagnose...Ch. 17.7 - Describe the benefits of creating transgenic...Ch. 17.7 - Prob. 2LOCh. 17.7 - Evaluate issues on each side of the transgenic...Ch. 17 - Prob. 1DACh. 17 - Prob. 2DACh. 17 - Prob. 1IQCh. 17 - Prob. 2IQCh. 17 - You study a gene known to be important in the...Ch. 17 - What is the basis of separation of different DNA...Ch. 17 - Prob. 3UCh. 17 - FISH analysis of a breast tumor biopsy for HER2...Ch. 17 - In terms of studying gene function, what is the...Ch. 17 - The Ti plasmid of Agrobacterium usually induces...Ch. 17 - Prob. 1ACh. 17 - Which of the following statements is accurate for...Ch. 17 - Prob. 3ACh. 17 - Many human proteins, such as hemoglobin, are only...Ch. 17 - Amyloid beta is a proteolytic product of a protein...
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