Principles of Biology
2nd Edition
ISBN: 9781259875120
Author: Robert Brooker, Eric P. Widmaier Dr., Linda Graham Dr. Ph.D., Peter Stiling Dr. Ph.D.
Publisher: McGraw-Hill Education
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Chapter 17.2, Problem 4CC
Summary Introduction
To determine:
The number of cells in the colony after 16 hours of division, when the single cell is placed on the plate. The bacterial strain divides every 30 minutes.
Introduction
The bacterial cell divides at a regular interval of time. The bacterial growth represents the total number of live cells present in a bacterial population.
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A culture with approximately 4x105 cells/mL were incubated. After 10 hours, the number of cells had increased to 5x109.
a) How long was the generation time in minutes?b) How many generations have occurred?
A culture with approximately 3x104 cells/mL were incubated. After 3 hours, the number of cells had increased to 6x105
a) How many generations have occurred?
In the experiment by Bernard Davis, bacterial F+cells and F- cells were growing while separated by a filter. Filter pores allowed the passage of the liquid medium but not the bacteria cells. As a result:
1) prototype colonies grew well on minimal medium
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3)F- cells were converted F+ cells despite the physical separation
4)F+ cells were not converted to F- cells because of the physical separation
5) F- cells were not converted to F+ cells because of the physical separation
6)there was no growth of prototypes on minimal medium
Chapter 17 Solutions
Principles of Biology
Ch. 17.1 - Prob. 1CCCh. 17.1 - Prob. 1BCCh. 17.1 - Prob. 1TYKCh. 17.1 - The step in the viral cycle that follows...Ch. 17.2 - Prob. 1CCCh. 17.2 - Prob. 1BCCh. 17.2 - Describe how the loop domains are held in place.Ch. 17.2 - Prob. 3CCCh. 17.2 - Prob. 4CCCh. 17.2 - Prob. 1TYK
Ch. 17.2 - Prob. 2TYKCh. 17.2 - Prob. 3TYKCh. 17.3 - Prob. 1CCCh. 17.3 - Prob. 1BCCh. 17.3 - Prob. 2CCCh. 17.3 - Prob. 1TYKCh. 17.3 - Prob. 2TYKCh. 17 - Prob. 1TYCh. 17 - Which of the following does hot describe a typical...Ch. 17 - Prob. 3TYCh. 17 - Prob. 4TYCh. 17 - Prob. 5TYCh. 17 - Genetic diversity is not maintained in bacterial...Ch. 17 - Prob. 7TYCh. 17 - Prob. 8TYCh. 17 - Prob. 9TYCh. 17 - Prob. 10TYCh. 17 - How are viruses similar to and different from...Ch. 17 - Describe the three mechanisms of gene transfer in...Ch. 17 - Prob. 3CCQCh. 17 - Prob. 1CBQCh. 17 - Conjugation is sometimes called bacterial mating....
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- Describe the four phases of bacterial cell division. Where would horizontal gene transfer occur? Where would sporogenesis occur? If antibiotics generally target cells which are growing and dividing as fast as they can, when would antibiotics be most effective? If a culture had 4 cells to begin with and has a generation time of 60 minutes, how long would it take to get 1,048,576 cells?arrow_forwardYou inoculated a culture with an initial cell count of 6.5x10^3 cells. The generation time for this organism is 25 minutes. You grew the culture for 10 hours. a) How many generations occurred?b) How many cells will be present after the 10 hours?arrow_forwardBacillus cereus divides every 30 minutes. You inoculate a culture with exactly 100 bacterial cells. After 3 hours, how many bacteria are present?arrow_forward
- What is not part of a Bacterial cell base on the picture?arrow_forwardGive only typing answer with explanation and conclusion 1. e coli divides every 20 mins. assuming you start with one cell, how many cells are estimated to be in a 24 hour culture?arrow_forwardWhy would you want to limit the number of times you passage a cell culture line?arrow_forward
- Suppose you count 35 bacterial cells from a solution that has gone through the following serial dilutions: two 10-fold dilutions, followed by a 5-fold dilution, followed by 2-fold dilution. What should be the bacterial count from the original solution? Show your workarrow_forwardWhen analyzing your results after performing a serial dilution, you obtain an average of 40 colonies on your 1 x 106 plate. How many cells were in the original sample?arrow_forwardCentrifugation at 4000rpm for 15 minutes will results in which enriched components of the isolation process? a.Proteins and cellular debris b.Cells and nuclei c.Mitochondrion and ribosomesarrow_forward
- In a serial dilution, you plate 1 ml of a 1/1000 dilution of a bacterial culture and find 25 colonies the next day. What was the concentration of cells in the original culture? A) 25 cells/ml B) 0.000025 cells/ml C) 25,000 cells/ml D) 26 cells/ml E) 0.025 cells/mlarrow_forwardIf a flask is inoculate with 10 E. coli cells. After 6 hours you now have 200,000 cells. determine both n (number of generations) and g (generation time) in minutes? A culture of S. epidermidis is growing. It divides every 20 min. After 4 hours, how many generations occurred?arrow_forwardThere are 10 E. coli cells in a test tube. After 4 generations in exponential phase, how many E. coli are there?arrow_forward
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Bacterial Genomics and Metagenomics; Author: Quadram Institute;https://www.youtube.com/watch?v=_6IdVTAFXoU;License: Standard youtube license