Investigating Biology Laboratory Manual (9th Edition)
9th Edition
ISBN: 9780134473468
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Jane B. Reece, Judith Giles Morgan, M. Eloise Brown Carter
Publisher: PEARSON
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Chapter 18, Problem 15TYU
WRITE ABOUT A THEME: INTERACTIONS In a Short essay (100-150 words), discuss how the proccsscs shown in Figure 18.2 are examples of feedback mechanisms regulating biological systems in bacterial cells.
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Shown below is a schematic diagram of the E. coli leader peptidase (Lep), which has several basic amino acids in a cytoplasmic loop. Propose a mutant of Lep that would be a test of the “inside positive” rule for the orientation of proteins in membranes.
Direction: Study the given amino acid sequence and DNA sequence of the
Amino Acid Sequence: LEU-ISC-PRO-PRO-PHE-ILE-LEU-LEU-SER-HIS-LEU-LEU-SER
What's More
Activity 3.1 Check and Relate
listed organisms.
Cat
DNA Sequence: TTAATCCCCCCGTTTATCCTACTTTCCCATCTACTAAGT
Am no Acid Sequence: LEU-ISC-PRO-PRO-PHE-ILE-LEU-EU-SER-ARG-LEU-LEU.AD
DNA Sequence: CTTATCCCCCCGTTTATCCTACTTTCCCGTCTACTTCGT
Shark
Amino Acid Sequence: LEU-ISC-PRO-PRO-PHE-ILE-LEU-LEU-SER-HIS-VAL-VAL-SER
DNA Sequence: CTAATCCCCCCGTTTATCCTACTTTCCCATGTAGTAAGT
Colphin
Amino Acid Sequence: LEU-ISO-PRO-PRO-PHE-LE-LEU-LEU-SER-ARG-LEU-LEU-ARG
DNA Sequence: CTAATCCCCCCGTTTATCCTACTTTCCCGTCTACTTCGT
Lizard
Amino Add Sequence: ISO-4SO ASP-GLN-PHE-ILE-LEU-HIS-SER-ARG-LEU-LEU-ARG
DNA Sequence: ATTATCGACCAGTTTATCCTACATTCCCGTCTACTTCGT
Sponge
Activity Questions:
1. Which organisms are closely related to each other? How are they
related?
2.
What does this tell us about the organisms and their ancestors?
3.
How amino acid sequences and DNA…
In the absence of tension, MscL channel has an energy gap separating the closed and open states Eo = 50 kT. MscL opening is associated with a 20 nm2 in-plane protein expansion (DA). Using Excel plot its open probability (Po) as a function of membrane tension and paste the graph below. What will be the open probabilities of two MscL channels if one situates at the pole of the bacterial cell above, another in the middle?
Chapter 18 Solutions
Investigating Biology Laboratory Manual (9th Edition)
Ch. 18.1 - How does binding of the trp corepressor to the trp...Ch. 18.1 - Describe the binding of RNA Polymerase,...Ch. 18.1 - WHAT IF? A certain mutation in E. coli changes...Ch. 18.2 - In general, what are the effects of histone...Ch. 18.2 - MAKE CONNECTIONS Speculate about whether the same...Ch. 18.2 - Compare the roles of general and specific...Ch. 18.2 - Once mRNA encoding a particular protein reaches...Ch. 18.2 - WHAT IF? Suppose you compared the nucleotide...Ch. 18.3 - Compare miRNAs and siRNAs, including their...Ch. 18.3 - WH AT IF? Suppose the mRNA being degraded in...
Ch. 18.3 - MAKE CONNECTIONS Inactivation of one of the X...Ch. 18.4 - MAKE CONNECTIONS As you learned in Chapter 12,...Ch. 18.4 - MAKE CONNECTIONS Explain how the signaling...Ch. 18.4 - How do fruit fly maternal effect genes determine...Ch. 18.4 - Prob. 4CCCh. 18.5 - Prob. 1CCCh. 18.5 - Under what circumstances is cancer considered to...Ch. 18.5 - MAKE CONNECTIONS The p53 protein can activate...Ch. 18 - Compare and contrast the roles of a corepressor...Ch. 18 - Describe what must happen in a cell for a gene...Ch. 18 - Why are miRNAs called noncoding RNAs? Explsin how...Ch. 18 - Describe the two main processes that cause...Ch. 18 - Compare the usual functions of proteins encoded by...Ch. 18 - If a particular operon encodes enzymes for making...Ch. 18 - Muscle cells differ from nerve cells mainly...Ch. 18 - The functioning of enhancers is an example of (A)...Ch. 18 - Cell differentiation always involves (A)...Ch. 18 - Which of the following is an example of...Ch. 18 - What would occur if the repressor of an inducible...Ch. 18 - Absence of bicoid in mRNA from a Drosophila egg...Ch. 18 - Which of the following statements about the DNA in...Ch. 18 - Within a cell, the amount of protein made using a...Ch. 18 - Prob. 10TYUCh. 18 - draw it The diagram below shows five genes,...Ch. 18 - Prob. 12TYUCh. 18 - Prob. 13TYUCh. 18 - SCIENCE. TECHNOLOGY, AND SOCIETY Trace amounts of...Ch. 18 - WRITE ABOUT A THEME: INTERACTIONS In a Short essay...Ch. 18 - SYNTHESIZE YOUR KNOWLEDGE The flashlight fish has...
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- VISUALIZE Sketch a simple flow diagram that shows the relationships among the following: RNA, translation, DNA, transcription, and polypeptide.arrow_forwardQOD #6 Directions: Label the illustration below using the following terms: MRNA, ÉRNA, rRNA/ribosome, transcription, translation, codon, polypeptide chain/protein. Each term will only be used once.arrow_forwardIn the absence of tension, MscL channel has an energy gap separating the closed and open states Eo = 50 kT. MscL opening is associated with a 20 nm2 in-plane protein expansion (change in A). Plot its open probability (Po) as a function of membrane tension. What will be the open probabilities of two MscL channels if one situates at the pole of the bacterial cell above, another in the middle?arrow_forward
- Or. Foyez Samar The volume enclosed by the plasma membrane of plant cells is often much larger the c corresponding volume in animal cells. The most regsengble explanation for this observation Is that A) plant cells are capable of having a much higher surface-to-volume ratio than animal cells. B) plant cells have a much more highly convoluted (folded) plasma membrane than animal cens. C) plant cells contain a large vacuole that reduces the volume of the cytoplasm. D) animal cells are more spherical, whereas plant cells are elongated. 12 A uarrow_forwardHelp Pleasearrow_forwardDraw out protein transport in the cell. Make sure to include all steps involved in transport through the secretory and endocytic pathways (including ER resident protein retrieval, transport to lysosome), transport in and out of the nucleus, and import into mitochondria. Include all details of vesicle transport (protein coat, SNARES, Rab) in your drawingarrow_forward
- Please don't provide handwriting solutionarrow_forwardview View Help O Editing AaBbCc AaBbCc No Spacing AaBbCc AaBbe Normal Heading 1 Heading Paragraph Styles 34) The RNA that has an anticodon and attaches to a specific amino acid is RNA. 35) T/F Buffers cause abrupt and large changes the pH of the body by releasing or binding H+ ions. 36) What is a dynamic equilibrium of your internal environment termed? 37) are membrane junctions that distribute tension across a sheet of cells. 38) T/F About 60% to 80% of the volume of most living cells consists of organic compounds. ictions: Onarrow_forwardthe game. And the game officially started! The game will work by team. Sakuragi and his team are placed in small submarine shrunk down to microscopic size and injected into a stained living ceili. The teams goai is to find out what is happening in the area because the object is slowly occupying the space where there are many creatures are living. They started their path on the fluid. As they continue their journey, they reached in a transparent layer. In order to make the navigational adjustment, 1. they need to identify this substance. What could be this thing? They scanned the area using an ultra telescope designed by the company that useful for their goal. Upon using the tool they have found out that it contains a wide variety of biological molecules magnification micro 2 There are polar and non - polar region. What are these biomolecules? They tried to get insidearrow_forward
- Briefly describe the experiment carried out to demonstrate that proteins are capable of lateral movement in cell membranes.arrow_forwardThe amino acid of a transmembrane protein was analyzed with a hydrophobicity (Kite-Doolittle) plot as shown below. Based on the graph on the left below how many times does the protein predicted pass through the membrane? TIBS 15-MARCH 1990 RVMV. Raw format file: RVMV. Protein Length: 318 Check: 7395 4. RVMV. Raw format file: RVMV. Protein Length: 318 Check: 7395 3 3 2- 2 -2- --3 -2 50 100 150 200 250 300 50 100 150 200 250 300 Residue Number Residue Number Figure 2 Figure 4 The Kyte-Doolittle (KD)12 hydrophobicity plot (w 9) of the M-chain of Rsp. viridis. The Sieved-Kyte-Doolittle (SKD)* hydrophobicity plot (w = 9) of the M-chain of Rsp. viridis. 1 time 3 time 5 times 7 times Other Mesh Sievedarrow_forwardCheck the box under each molecule for which the statement in the first column is true. statement It transfers instructions for making a new protein from the nucleus to ribosomes out in the cytoplasm. When attached to a ribosome, each codon specifies the next amino acid in a protein under construction. It usually contains several thousand adenine, uracil, cytosine, and guanine residues. It's secreted by the adrenal cortex, but transported throughout the body to do its job. When combined with a number of different proteins, it forms a large complex in the cytoplasm responsible for synthesizing new proteins. transfer messenger ribosomal RNA RNA RNA 0 [] n Uarrow_forward
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