Investigating Biology Laboratory Manual (9th Edition)
9th Edition
ISBN: 9780134473468
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Jane B. Reece, Judith Giles Morgan, M. Eloise Brown Carter
Publisher: PEARSON
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Textbook Question
Chapter 18, Problem 6TYU
What would occur if the repressor of an inducible operon were mutated so it could not bind the operator?
- (A) irreversible binding of the repressor to the promoter
- (B) reduced transcription of the operon's genes
- (C) buildup of a substrate for the pathway controlled by the Operon
- (D) continuous transcription of the operon's genes
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A mutation that inactivates the repressor gene of the lac operon results in (a) the continuous transcription of the structural genes (b) no transcription of the structural genes (c) the binding of the repressor to the operator (d) no production of RNA polymerase (e) no difference in the rate of transcription
In the trp operon, if a mutation occurred in the gene for the repressor so that it could bind to the operator even in the absence of tryptophan____________.
a) the inducer cannot bind trpO, so operon gene transcription occurs
b) the active repressor cannot bind trpO, so operon gene transcription is attenuated
c)the active repressor binds tpO, so operon gene transcription is always repressed
d) the repressor binds the corepressor, and operon gene transcription occurs.
If a researcher moves the promoter for the lac operon to the region between the beta
galactosidase (lacZ) gene and the permease (lacY) gene, which of the following results would
you expect?
A) The three genes of the lac operon will be expressed normally.
B) The repressor will not be able to bind to the operon.
C) The operon will still transcribe the lacZ and lacY genes, but the mRNA will not be translated.
D) LacZ will not be transcribed and Beta galactosidase will not be produced.
Chapter 18 Solutions
Investigating Biology Laboratory Manual (9th Edition)
Ch. 18.1 - How does binding of the trp corepressor to the trp...Ch. 18.1 - Describe the binding of RNA Polymerase,...Ch. 18.1 - WHAT IF? A certain mutation in E. coli changes...Ch. 18.2 - In general, what are the effects of histone...Ch. 18.2 - MAKE CONNECTIONS Speculate about whether the same...Ch. 18.2 - Compare the roles of general and specific...Ch. 18.2 - Once mRNA encoding a particular protein reaches...Ch. 18.2 - WHAT IF? Suppose you compared the nucleotide...Ch. 18.3 - Compare miRNAs and siRNAs, including their...Ch. 18.3 - WH AT IF? Suppose the mRNA being degraded in...
Ch. 18.3 - MAKE CONNECTIONS Inactivation of one of the X...Ch. 18.4 - MAKE CONNECTIONS As you learned in Chapter 12,...Ch. 18.4 - MAKE CONNECTIONS Explain how the signaling...Ch. 18.4 - How do fruit fly maternal effect genes determine...Ch. 18.4 - Prob. 4CCCh. 18.5 - Prob. 1CCCh. 18.5 - Under what circumstances is cancer considered to...Ch. 18.5 - MAKE CONNECTIONS The p53 protein can activate...Ch. 18 - Compare and contrast the roles of a corepressor...Ch. 18 - Describe what must happen in a cell for a gene...Ch. 18 - Why are miRNAs called noncoding RNAs? Explsin how...Ch. 18 - Describe the two main processes that cause...Ch. 18 - Compare the usual functions of proteins encoded by...Ch. 18 - If a particular operon encodes enzymes for making...Ch. 18 - Muscle cells differ from nerve cells mainly...Ch. 18 - The functioning of enhancers is an example of (A)...Ch. 18 - Cell differentiation always involves (A)...Ch. 18 - Which of the following is an example of...Ch. 18 - What would occur if the repressor of an inducible...Ch. 18 - Absence of bicoid in mRNA from a Drosophila egg...Ch. 18 - Which of the following statements about the DNA in...Ch. 18 - Within a cell, the amount of protein made using a...Ch. 18 - Prob. 10TYUCh. 18 - draw it The diagram below shows five genes,...Ch. 18 - Prob. 12TYUCh. 18 - Prob. 13TYUCh. 18 - SCIENCE. TECHNOLOGY, AND SOCIETY Trace amounts of...Ch. 18 - WRITE ABOUT A THEME: INTERACTIONS In a Short essay...Ch. 18 - SYNTHESIZE YOUR KNOWLEDGE The flashlight fish has...
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- If β-galactosidase is expressed when lactose is absent, what does this suggest about the lac operon? a) There is a mutation in the repressor protein b) There is a mutation in the catabolite activator protein c) There is a mutation in the promoter region d) There is a mutation in the luxI proteinarrow_forward. If a particular operon encodes enzymes for makingan essential amino acid and is regulated like thetrp operon, then(A) the amino acid inactivates the repressor.(B) the repressor is active in the absence of theamino acid.(C) the amino acid acts as a corepressor.(D) the amino acid turns on transcription of the operonarrow_forwardThe trp operon, found in E. coli, is a group of genes that encode biosynthetic enzymes for the amino acid tryptophan. (i) Draw a schematic diagram showing the components involved in regulation of the tryptophan operon. (ii) How does the regulation of the trp operon differ from the regulation of the lac operon? Explain.arrow_forward
- Which option is true when considering the regulation of cAMP and the Lac operon. If bacteria are in a medium containing low glucose, but suddenly glucose concentration in the medium increases by ten times, then we expect that cAMP...... a) Will increase inside the cell. b) Will not increase inside the cell.arrow_forwardHow will transcription of the E. coli trp (tryptophan) operon be affected by the following manipulations of the leader region of the trp mRNA?(a) Increasing the distance (number of bases) between the leader peptide gene and region 2(b) Increasing the distance between region 2 and region 3(c) Removing region 4(d) Changing several nucleotides in region 3 so that in can base pair with region 4 but not with region 2arrow_forwardThe attenuation mechanism that helps block expression of the tryptophan operon requires all of the following, except A) O MRNA transcript of the trp leader sequence B) Oaribosome stalled at the stop codon of the trp leader transcript C) O formation of an attenuator loop D) O low intracellular levels of tryptophanarrow_forward
- In the presence of lactose and the promoter sequence was mutated such that the RNA polymerase can no longer bind, the structural genes will:[ Select ] ["No longer be expressed", "Be constantly expressed", "Be deleted from the operon"] In the absence of lactose and the lacI repressor gene is mutated such that the repressor protein product can no longer bind to the operator, the structural genes will: [ Select ] ["No longer be expressed", "Be constantly expressed", "Be deleted from the operon"] In the presence of lactose and the lacI repressor gene is mutated such that the repressor protein product can no longer bind to the operator, the structural genes will: [ Select ] ["No longer be expressed", "Be constantly expressed", "Be deleted from the operon"]arrow_forwardWhat is the phenotype of an E. coli strain with a mutation in the lac operator that allows the lac repressor to irreversibly bind the operator? Assume glucose is absent. 1S A) O Transcription of the operon is very high whether lactose is present or not. B) O Transcription of the operon is very low whether lactose is present or not. C) O Transcription of the operon is high in the presence of lactose, and low in its absence. D) O Transcription of the operon is low in the presence of lactose, and high in its absence.arrow_forwardWhat would happen to the regulation of the tryptophan operon in bacterial cells that express a mutant form of the tryptophan repressor that (1) cannot bind to DNA, (2) cannot bind tryptophan, or (3) binds to DNA even in the absence of tryptophan?arrow_forward
- High levels of transcription of the lactose operon require all of the following, EXCEPT: A) O the presence of lactose B) O high levels of CAMP (cyclic AMP) C) O the absence of glucose D) O the formation of allolactose E) O A-D are all requiredarrow_forwardIn the presence of tryptophan, and a mutation in the allosteric domain that abolishes tryptophan binding______. a) the active repressor cannot bind trp), so operon gene transription is attenuated b) the inactive repressor cannot bind trpO, so operon gene transcription occurs] c) the active repressor binds trpP, so operon gene trasncription is repressed, the inducer cannot bind trpO, so gene transcription occurs d) the repressor binds to the corepressor, and an operon gene transcription occursarrow_forwardWhich of the following statements is not true of both the LacI and TrpR repressor proteins?a) an allosteric effector molecule changes the DNA-binding activity of the proteinb) transcription is blocked when the repressor protein is bound to the operatorc) the repressor protein is trans-actingd) the allosteric effector causes the repressor to release from the operatore) the operon regulated by the repressor produces the allosteric effector moleculearrow_forward
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