Chapter 18, Problem 18.15P

(a)

Interpretation Introduction

Interpretation:

Concentration of solution with $8.83\times {10}^4{\text{ M}}^{-1}{\text{cm}}^{-1}$ molar absorptivity at a wavelength of $280\text{ nm}$ and absorbance of 0.244 has to be calculated.

Concept Introduction:

Expression to compute absorbance from molar absorptivity as per Beer’s law is as follows:

  $A=\epsilon \cdot b\cdot c$

Here,

$\epsilon$ denotes molar absorptivity of species capable to absorb in ${\text{M}}^{-1}{\text{cm}}^{-1}$.

$b$ denotes path length in centimeters.

$c$ denotes concentration or molarity of species capable to absorb.

$A$ denotes absorbance.

$T$ denotes transmittance.

(b)

Interpretation Introduction

Interpretation:

Concentration of apotransferrin in $\text{g/L}$ has to be calculated.

Concept Introduction:

Refer to part (a).