Propose an experiment that can be done easily with a DNA microarray or RNA-seq that would have required years to do before these technologies were developed.

Question

Want to see more full solutions like this?

Answer 1

Textbook Question

Chapter 18, Problem 1CHI

Propose an experiment that can be done easily with a DNA microarray or RNA-seq that would have required years to do before these technologies were developed.

Expert Solution & Answer

Summary Introduction

DNA microarrays and RNA-sequencing are the two techniques used to study the transcriptomes. Both these techniques are involved in assessing the expression of genes as a measure of individual transcripts (mRNA) of the gene. The process in which the sequencing of total mRNA is carried out directly is referred to as RNA sequencing. This technique replaced the microarrays and is used as a transcriptomic tool. This is because RNA sequencing provides the data without the limitations that are encountered in the microarray analysis.

Explanation of Solution

Both the microarray as well as the RNA-sequencing methodology is found to be highly parallel in nature. This is because, in these techniques, a large number of genes is examined at once. In the past, if a researcher was interested in studying the survival of heat stress, he or she would examine the genes one-by-one for its expression in the cells which are exposed to heat by the Northern blot or reverse transcriptase-PCR.

In addition to this, the researcher would have probably made a series of knock out mutants. This is carried out with the deletion of single and multiple genes. This effort is made to detect the function of the products of genes and their possible interactions. By contrast, the RNA-sequencing and microarray techniques analyze all the genes at the transcription level in one experiment itself. But of course, a sequenced genome is required for these methodologies.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Students have asked these similar questions

Propose an experiment that can be done easily with a DNA microarray or RNA-seq that would have required years to do before these technologies were developed.

Discuss the advantages of next-generation sequencing technologies.

Briefly describe two scientific problems that can be addressed using the data from DNA barcoding experiments.

Answer 2

Textbook Question

Chapter 18, Problem 1CHI

Propose an experiment that can be done easily with a DNA microarray or RNA-seq that would have required years to do before these technologies were developed.

Expert Solution & Answer

Summary Introduction

DNA microarrays and RNA-sequencing are the two techniques used to study the transcriptomes. Both these techniques are involved in assessing the expression of genes as a measure of individual transcripts (mRNA) of the gene. The process in which the sequencing of total mRNA is carried out directly is referred to as RNA sequencing. This technique replaced the microarrays and is used as a transcriptomic tool. This is because RNA sequencing provides the data without the limitations that are encountered in the microarray analysis.

Explanation of Solution

Both the microarray as well as the RNA-sequencing methodology is found to be highly parallel in nature. This is because, in these techniques, a large number of genes is examined at once. In the past, if a researcher was interested in studying the survival of heat stress, he or she would examine the genes one-by-one for its expression in the cells which are exposed to heat by the Northern blot or reverse transcriptase-PCR.

In addition to this, the researcher would have probably made a series of knock out mutants. This is carried out with the deletion of single and multiple genes. This effort is made to detect the function of the products of genes and their possible interactions. By contrast, the RNA-sequencing and microarray techniques analyze all the genes at the transcription level in one experiment itself. But of course, a sequenced genome is required for these methodologies.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 3

Textbook Question

Chapter 18, Problem 1CHI

Propose an experiment that can be done easily with a DNA microarray or RNA-seq that would have required years to do before these technologies were developed.

Expert Solution & Answer

Summary Introduction

DNA microarrays and RNA-sequencing are the two techniques used to study the transcriptomes. Both these techniques are involved in assessing the expression of genes as a measure of individual transcripts (mRNA) of the gene. The process in which the sequencing of total mRNA is carried out directly is referred to as RNA sequencing. This technique replaced the microarrays and is used as a transcriptomic tool. This is because RNA sequencing provides the data without the limitations that are encountered in the microarray analysis.

Explanation of Solution

Both the microarray as well as the RNA-sequencing methodology is found to be highly parallel in nature. This is because, in these techniques, a large number of genes is examined at once. In the past, if a researcher was interested in studying the survival of heat stress, he or she would examine the genes one-by-one for its expression in the cells which are exposed to heat by the Northern blot or reverse transcriptase-PCR.

In addition to this, the researcher would have probably made a series of knock out mutants. This is carried out with the deletion of single and multiple genes. This effort is made to detect the function of the products of genes and their possible interactions. By contrast, the RNA-sequencing and microarray techniques analyze all the genes at the transcription level in one experiment itself. But of course, a sequenced genome is required for these methodologies.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 4

Textbook Question

Chapter 18, Problem 1CHI

Propose an experiment that can be done easily with a DNA microarray or RNA-seq that would have required years to do before these technologies were developed.

Expert Solution & Answer

Summary Introduction

DNA microarrays and RNA-sequencing are the two techniques used to study the transcriptomes. Both these techniques are involved in assessing the expression of genes as a measure of individual transcripts (mRNA) of the gene. The process in which the sequencing of total mRNA is carried out directly is referred to as RNA sequencing. This technique replaced the microarrays and is used as a transcriptomic tool. This is because RNA sequencing provides the data without the limitations that are encountered in the microarray analysis.

Explanation of Solution

Both the microarray as well as the RNA-sequencing methodology is found to be highly parallel in nature. This is because, in these techniques, a large number of genes is examined at once. In the past, if a researcher was interested in studying the survival of heat stress, he or she would examine the genes one-by-one for its expression in the cells which are exposed to heat by the Northern blot or reverse transcriptase-PCR.

In addition to this, the researcher would have probably made a series of knock out mutants. This is carried out with the deletion of single and multiple genes. This effort is made to detect the function of the products of genes and their possible interactions. By contrast, the RNA-sequencing and microarray techniques analyze all the genes at the transcription level in one experiment itself. But of course, a sequenced genome is required for these methodologies.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 5

Textbook Question

Answer 6

Textbook Question

Answer 7

Expert Solution & Answer

Summary Introduction

DNA microarrays and RNA-sequencing are the two techniques used to study the transcriptomes. Both these techniques are involved in assessing the expression of genes as a measure of individual transcripts (mRNA) of the gene. The process in which the sequencing of total mRNA is carried out directly is referred to as RNA sequencing. This technique replaced the microarrays and is used as a transcriptomic tool. This is because RNA sequencing provides the data without the limitations that are encountered in the microarray analysis.

Explanation of Solution

Both the microarray as well as the RNA-sequencing methodology is found to be highly parallel in nature. This is because, in these techniques, a large number of genes is examined at once. In the past, if a researcher was interested in studying the survival of heat stress, he or she would examine the genes one-by-one for its expression in the cells which are exposed to heat by the Northern blot or reverse transcriptase-PCR.

In addition to this, the researcher would have probably made a series of knock out mutants. This is carried out with the deletion of single and multiple genes. This effort is made to detect the function of the products of genes and their possible interactions. By contrast, the RNA-sequencing and microarray techniques analyze all the genes at the transcription level in one experiment itself. But of course, a sequenced genome is required for these methodologies.

Answer 8

Expert Solution & Answer

Summary Introduction

DNA microarrays and RNA-sequencing are the two techniques used to study the transcriptomes. Both these techniques are involved in assessing the expression of genes as a measure of individual transcripts (mRNA) of the gene. The process in which the sequencing of total mRNA is carried out directly is referred to as RNA sequencing. This technique replaced the microarrays and is used as a transcriptomic tool. This is because RNA sequencing provides the data without the limitations that are encountered in the microarray analysis.

Explanation of Solution

Both the microarray as well as the RNA-sequencing methodology is found to be highly parallel in nature. This is because, in these techniques, a large number of genes is examined at once. In the past, if a researcher was interested in studying the survival of heat stress, he or she would examine the genes one-by-one for its expression in the cells which are exposed to heat by the Northern blot or reverse transcriptase-PCR.

In addition to this, the researcher would have probably made a series of knock out mutants. This is carried out with the deletion of single and multiple genes. This effort is made to detect the function of the products of genes and their possible interactions. By contrast, the RNA-sequencing and microarray techniques analyze all the genes at the transcription level in one experiment itself. But of course, a sequenced genome is required for these methodologies.

Answer 9

Expert Solution & Answer

Answer 10

Expert Solution & Answer

Answer 11

Summary Introduction

DNA microarrays and RNA-sequencing are the two techniques used to study the transcriptomes. Both these techniques are involved in assessing the expression of genes as a measure of individual transcripts (mRNA) of the gene. The process in which the sequencing of total mRNA is carried out directly is referred to as RNA sequencing. This technique replaced the microarrays and is used as a transcriptomic tool. This is because RNA sequencing provides the data without the limitations that are encountered in the microarray analysis.

Answer 12

Explanation of Solution

Both the microarray as well as the RNA-sequencing methodology is found to be highly parallel in nature. This is because, in these techniques, a large number of genes is examined at once. In the past, if a researcher was interested in studying the survival of heat stress, he or she would examine the genes one-by-one for its expression in the cells which are exposed to heat by the Northern blot or reverse transcriptase-PCR.

In addition to this, the researcher would have probably made a series of knock out mutants. This is carried out with the deletion of single and multiple genes. This effort is made to detect the function of the products of genes and their possible interactions. By contrast, the RNA-sequencing and microarray techniques analyze all the genes at the transcription level in one experiment itself. But of course, a sequenced genome is required for these methodologies.

Answer 13

Ch. 18.1 - MICRO INQUIRY What is the function of the 3-OH...Ch. 18.1 - MICRO INQUIRY Why is it important that identical...Ch. 18.2 - MICRO INQUIRY Which step (or steps) in this...Ch. 18.2 - Retrieve, Infer, Apply Why is the Sanger technique...Ch. 18.2 - Retrieve, Infer, Apply Explain the difference...Ch. 18.2 - Retrieve, Infer, Apply Why does reversible chain...Ch. 18.2 - Prob. 4RIA Ch. 18.2 - Retrieve, Infer, Apply Suggest a medical and an...Ch. 18.3 - Retrieve, Infer, Apply What is a phylotype?Ch. 18.3 - Retrieve, Infer, Apply NGS techniques are...

Propose an experiment that can be done easily with a DNA microarray or RNA-seq that would have required years to do before these technologies were developed.

Concept explainers

Videos

Explanation of Solution

Want to see more full solutions like this?

Chapter 18 Solutions