10th Edition

ISBN: 9781259281594

Author: Joanne Willey, Linda Sherwood Adjunt Professor Lecturer, Christopher J. Woolverton Professor

Publisher: McGraw-Hill Education

Concept explainers

Molecular techniques are methods employed in molecular biology, genetics, biochemistry, and biophysics to manipulate and analyze nucleic acids (deoxyribonucleic acid (DNA) and ribonucleic acid (RNA)), protein, and lipids. Techniques in molecular biology …

DNA Fingerprinting and Gel Electrophoresis

The genetic makeup of living organisms is shown by a technique known as DNA fingerprinting. The difference is the satellite region of DNA is shown by this process. Alex Jeffreys has invented the process of DNA fingerprinting in 1985. Any biological sampl…

Molecular Markers

A known DNA sequence or gene sequence is present on a chromosome, and it is associated with a specific trait or character. It is mainly used as a genetic marker of the molecular marker. The first genetic map was done in a fruit fly, using genes as the fi…

DNA Sequencing

The most important feature of DNA (deoxyribonucleic acid) molecules are nucleotide sequences and the identification of genes and their activities. This the reason why scientists have been working to determine the sequences of pieces of DNA covered under …

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Textbook Question

Chapter 18.3, Problem 3RIA

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Examine figure 18.8. How might metagenomics be used to isolate genes encoding a potentially new antibiotic?

Chapter 18.3, Problem 3RIA, Retrieve, Infer, Apply Examine figure 18.8. How might metagenomics be used to isolate genes encoding

Figure 18.8 Metagenomic analysis. DNA has been extracted directly from environments such as (a) bacterial mats at Yellowstone National Park, (b) the human colon, (c) cabbage white butterfly larvae, and (d) tube worms from hydrothermal vents. The DNA can be used to construct a metagenomic library and analyzed by Sanger sequencing (top); however, NGS is more likely to be used (bottom), thereby avoiding the need for genomic library construction.

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Chapter 18.3, Problem 2RIA

Chapter 18.4, Problem 1MI

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Paraphrase and add more information "This study will concentrate solely on the molecular characterization of seaweed samples collected on Panglao island inSouth-Central Luzon, Philippines. Molecular data will be limited to DNA sequences, with identification relying heavily on Genbank returns via blastN. The researchers would obtain their samples from the generated database and analyze the DNA barcode of each macroalgae. The researchers would also compare the previous list of discovered seaweeds from Panglao island to generate a new and updated version for this study. The research would no text end outside or to the neighboring islands around Panglao, Bohol. Neither would the researchers add or include specimens that did not appear in the database or originate from Panglao island. The data to be considered would also not discuss or include the entire taxonomic key of each available sample."

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Chapter 18 Solutions

Prescott's Microbiology

Ch. 18.1 - MICRO INQUIRY What is the function of the 3-OH...Ch. 18.1 - MICRO INQUIRY Why is it important that identical...Ch. 18.2 - MICRO INQUIRY Which step (or steps) in this...Ch. 18.2 - Retrieve, Infer, Apply Why is the Sanger technique...Ch. 18.2 - Retrieve, Infer, Apply Explain the difference...Ch. 18.2 - Retrieve, Infer, Apply Why does reversible chain...Ch. 18.2 - Prob. 4RIA Ch. 18.2 - Retrieve, Infer, Apply Suggest a medical and an...Ch. 18.3 - Retrieve, Infer, Apply What is a phylotype?Ch. 18.3 - Retrieve, Infer, Apply NGS techniques are...

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Solution Summary: The author describes metagenomics as a study of microbes in their natural habitats without culturing them. It is an important approach in determining the type of organism present in an environment.

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