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The joining of two or more DNA molecules from different sources generates a recombinant DNA. The joining of two DNA (deoxyribonucleic acid) molecules is the main part of gene cloning. The DNA molecules are cut by the restriction enzymes, also called restriction endonucleases.
Answer to Problem 1TYK
Correct answer:
The sequence of
Explanation of Solution
Justification/ Explanation for the correct answer:
Option (a) is the sequence of nucleotides. The restriction enzymes help in recognition of restriction sites on the DNA fragments and cut them into different fragments. These restriction sites contain sequences of nucleotides that are recognized by enzymes and are palindromic in nature. There is a number of restriction enzymes identified for cutting DNA at specific restriction sites. Hence, option (a) is correct.
The explanation for the incorrect answers:
Option (b) is the length of DNA molecule. The activity of restriction enzymes depends on the presence of restriction sites on the DNA molecule. A long DNA molecule cannot be cut by an enzyme, if it does not contain particular recognition sites. So, it is an incorrect option.
Option (c) is whether it is closer to 5’end or 3´end of DNA molecule. The restriction enzymes recognize the palindromic sequence of DNA sequences, which can be read in both sides, from 5’ to 3´ and 3´ to 5´ ends. So, it is an incorrect option.
Option (d) is the number of copies of DNA molecule in a bacterial cell. A large number of copies of DNA cannot be cleaved by restriction enzymes without any restriction sites. So, it is an incorrect option.
Option (e) is the location of a start codon in a gene. Start codon only helps in the initiation of translation and protein synthesis. So, it is an incorrect option.
Hence, options (b), (c), (d) and (e) are incorrect.
Restriction sites are the sequence of DNA fragments which may vary in number and are recognized by a specific restriction endonuclease. The enzyme endonuclease cleaves the DNA fragment generating small fragments with sticky ends or blunt ends.
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Chapter 18 Solutions
Biology: The Dynamic Science (MindTap Course List)
- What are the sticky ends of the restriction fragments? Select one: a. The surfaces of sticky ends contain matching base pairs, allowing fragments to splice. b. The surfaces of sticky ends have glue like substance that allow fragments to splice. c. The surfaces of sticky ends contain the exact same nucleotides, allowing fragments to bond. d. The surfaces of sticky ends have velcro like structure, allowing fragments to bond.arrow_forwardA DNA fingerprint is based on _____. a repeating sequences found on noncoding regions b the presence of restriction enzymes c a single repeating region d repeating sequences found on coding regionsarrow_forwardPut the following tasks in the order they would occur during a DNA cloning experiment. a. using DNA ligase to seal DNA fragments into vectors b. using a probe to identify a clone in the library c. sequencing the DNA of the clone d. making a DNA library of clones e. cutting genomic DNA with restriction enzymesarrow_forward
- Cloning Genes Is a Multistep Process The following DNA sequence contains a six-base sequence that is a recognition and cutting site for a restriction enzyme. What is this sequence? Which enzyme will cut this sequence? (See Figure 13.5 for help.) 5 CCGAGGAAGCTTAC 3 3 GGCTCCTTCGAATG 5arrow_forwardA contig is a. a set of molecular markers used in gene mapping. b. a set of overlapping fragments that form a continuous stretch of DNA. c. a set of fragments generated by a restriction enzyme. d. a small DNA fragment used in sequencing.arrow_forwardIn genetic engineering, can we insert the gene from human to E. coli (bacteria)? Explain your answer.arrow_forward
- Put the following tasks in the order they would occur during a DNA cloning experiment. a. using DNA ligase to seal DNA fragments into vectors b. using a probe to identify a clone in the library c. sequencing the DNA of the clone d. making a DNA library of clones e. cutting genomic DNA with restriction enzymesarrow_forwardRestriction enzymes cut double-stranded DNA _____. a. at noncoding areas between genes b. between bacterial and viral DNAs c. at specific base sequences d. between purines and pyrimidines e. between promoter and operator DNA sequencesarrow_forwardThe restriction endonucleases used in recombinant DNA work: a. are synthesized by bacteria b. recognize sequences 14-16 bp long c. cut the DNA outside the recognition sequence d. all the above are truearrow_forward
- The activity of restriction enzymes may produce fragments with sticky ends. Sticky ends are a) a type of endonucleases. b) dephosphorylated CpG islands. c) unpaired nucleotides. d) double breaks with blunt ends.arrow_forwardA restriction enzyme digests DNA into fragments.term the technique used to check the progression of this enzyme and separate DNA fragments.arrow_forwardIn next-generation sequencing, which of these advances allows for massively parallel sequencing? a. Pieces of DNA are fixed to a surface, so we can tell which new nucleotides were added to each piece. b. DNA sequences are read in real-time as nucleotides are added to each piece. c. Each segment of the genome can be pieced back together through shotgun alignment d. Single molecules of DNA can be read without the need for amplification.arrow_forward
Human Heredity: Principles and Issues (MindTap Co...BiologyISBN:9781305251052Author:Michael CummingsPublisher:Cengage Learning