LIFE: THE SCIENCE OF BIOLOGY
11th Edition
ISBN: 9781319145125
Author: Sadava
Publisher: MAC HIGHER
expand_more
expand_more
format_list_bulleted
Question
Chapter 18.2, Problem 1R
Summary Introduction
To review:
The comparison of green fluorescent protein (GFP) as a reporter gene and antibiotic resistance gene with respect to detection of recombinant deoxyribonucleic acid (DNA) in host cells.
Introduction:
In recombinant technology, to know, which host cells contain recombinant DNA, a method of detection is needed. The problem is solved by reporter genes. Their expression is easy to visualize and hence they help to detect the host cells with recombinant DNA. The most common of these reporter genes is GFP. Another most common method is the use of selectable markers like antibiotic resistance gene.
Expert Solution & Answer
Trending nowThis is a popular solution!
Students have asked these similar questions
Explain:
Describe an electrochemical sensor assay method for rapid bacterial detection and identification. What are the principles and mechanisms involved?
functionalization of a sensor array with DNA oligonucleotide capture probes for ribosomal RNA (rRNA) species-specific sequences.
sandwich hybridization of target rRNA with the capture probe and a horseradish peroxidase linked DNA oligonucleotide detector probe.
Explain how bacteria are being detected using an electrochemical sensor
Explain the steps to transform a plasmid carrying a gene of interest into a competent host
cell using the heat shock treatment.
Cre-Lox system is used for site-specific modification of DNA for genetic engineering applications. The reporter gene construct
shown below is used to test the Cre-Lox system. Cells are transfected with the construct shown below and the activity of the
constructs is determined by visualizing the cells with a fluorescence microscope. Match the following conditions with the expected
cell observations. Hint: Make sure you note the position of the Start and Stop. The GFP and RFP genes shown do not have start
codons.
Cre/Lox Reporter Gene
ATG
LoxP
CMV-Pro
A. No Expression
GFP
Stop
B. GFP Only
LoxP
Absence of Cre
Cells treated with drug that induces expression of Cre
RFP
C. RFP Only
A. Image A
B. Image B
C. Image C
D Image D
express
?
D. RFP and GFP
Chapter 18 Solutions
LIFE: THE SCIENCE OF BIOLOGY
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- You have constructed a cDNA library in a phagemid vector.Describe how you would screen the library for a particulargene of interest. Describe methods using oligonucleotideand antibody probes.arrow_forwardPlace the stages of a CRISPR-cas9 gene editing workflow in the correct order (there is one incorrect stage) Design a set of targeting sequences using algorithms. Clonal isolation Transfect cells Synthesise DNA insert oligonucleotides Purify plasmids Sequence Clonal characterisation (analysis of phenotype) Select for correctly gene edited cells (using antibiotic resistance and/or colour production for example) Add green fluorescent protein gene sequence into plasmid to aid selection of correctly transfected cells. Clone into CRISPR-cas9 expression vectorarrow_forwardPlease place the stages of a CRISPR-cas9 gene editing workflow in the correct order below. Note, there is one incorrect and state why? Sequence Select for correctly gene edited cells (using antibiotic resistance and/or colour production for example) Clonal isolation Synthesise DNA insert oligonucleotides Clonal characterisation (analysis of phenotype) Add green fluorescent protein gene sequence into plasmid to aid selection of correctly transfected cells. Transfect cells Clone into CRISPR-cas9 expression vector Purify plasmids Design a set of targeting sequences using algorithms.arrow_forward
- Discuss some advantages and disadvantages of bacteriophage therapyin treating bacterial infections.arrow_forwardDescribe what will happen to bacteria with dysfunctional restriction modification system providing that restrictase (first scenario) or methylase (second scenario) is not functional when it gets attacked by phage or by another bacterial species. T: BIarrow_forwarddescribe how the bacteria should have the ability to be recovered back from the infected host for the purpose of experimentation including sequencing ,vitro culturing and polymerase chain reaction amplification.arrow_forward
- **Application of Abs in diagnosis and research: What is to be measured - Ag or Ab? Is the assay appropriate? Is the assay sensitive enough? Reporter strategies? Primary Ab vs 2nd Ab?arrow_forwardOutline the strategies commonly used to detect a cloned target gene in E. coli.arrow_forwardIn bacterial transformation, the purpose of having antibiotic within an agar plate is to: Select one: confirm which plasmids been have successfully ligated with a gene of interest. isolate bacteria which have been successfully transformed with the plasmid. indicate which plasmids were successfully digested by the endonuclease. act as a substrate which will be cleaved and produce a blue product when ligation is unsuccessful. show which plasmids contain the lacZ gene.arrow_forward
- describe the blue-white colony selection method in the recombinant colony screening, and present a brief argument for its use in biotechnology labs.arrow_forwardDiscuss how DNA extraction of microbial DNA directly can be done from infected tissue through an optimized protocol for use in nanopore sequencing.arrow_forwardexplain or describe the principle of immunoturbidimetric C3 Assay.arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
- Human Anatomy & Physiology (11th Edition)BiologyISBN:9780134580999Author:Elaine N. Marieb, Katja N. HoehnPublisher:PEARSONBiology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStaxAnatomy & PhysiologyBiologyISBN:9781259398629Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa StouterPublisher:Mcgraw Hill Education,
- Molecular Biology of the Cell (Sixth Edition)BiologyISBN:9780815344322Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter WalterPublisher:W. W. Norton & CompanyLaboratory Manual For Human Anatomy & PhysiologyBiologyISBN:9781260159363Author:Martin, Terry R., Prentice-craver, CynthiaPublisher:McGraw-Hill Publishing Co.Inquiry Into Life (16th Edition)BiologyISBN:9781260231700Author:Sylvia S. Mader, Michael WindelspechtPublisher:McGraw Hill Education
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:PEARSON
Biology 2e
Biology
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:OpenStax
Anatomy & Physiology
Biology
ISBN:9781259398629
Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:9780815344322
Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:9781260159363
Author:Martin, Terry R., Prentice-craver, Cynthia
Publisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Biology
ISBN:9781260231700
Author:Sylvia S. Mader, Michael Windelspecht
Publisher:McGraw Hill Education
Serology 101: Testing for IgG and IgM antibodies; Author: Beckman Coulter Dx;https://www.youtube.com/watch?v=LtqKB-qpJrs;License: Standard youtube license