Concept explainers
To review:
The reason behind a complementary deoxyribonucleic acid (cDNA) library constructed from human brain tissue being both similar to and different from a cDNA library constructed from the human pancreatic tissue.
Introduction:
A collection of different cloned deoxyribonucleic acid (DNA) sequences from an organism is called a gene library. There are usually two types of gene library, namely: genomic library and complementary DNA (cDNA) library, which depends on the source of DNA used. A collection of clones containing cDNA molecules that are usually synthesized from messenger ribonucleic acid (mRNA) molecules in a cell of an organism is called cDNA library. It contains only the coding regions of a genomic material of an organism, unlike genomic library which contains the whole genome of an organism.
Trending nowThis is a popular solution!
Chapter 18 Solutions
LIFE: THE SCIENCE OF BIOLOGY
- A protein has the following amino acid sequence: Met-Tyr-Asn-Val-Arg-Val-Tyr-Lys-Ala-Lys-Trp-Leu-Ile-His-Thr-Pro You wish to make a set of probes to screen a cDNA library for the sequence that encodes this protein. Your probes should be at least 18 nucleotides in length. Q. How many different probes must be synthesized to be certain that you will find the cDNA sequence that specifies the protein?arrow_forwardWhat sequence information about a gene is lackingin a cDNA library?arrow_forward. Explain why a long open reading frame suggests theexistence of a protein-coding exonarrow_forward
- A protein has the following amino acid sequence: Met-Tyr-Asn-Val-Arg-Val-Tyr-Lys-Ala-Lys-Trp-Leu-Ile-His-Thr-Pro You wish to make a set of probes to screen a cDNA library for the sequence that encodes this protein. Your probes should be at least 18 nucleotides in length. Q. Which amino acids in the protein should be used to construct the probes so that the least degeneracy results?arrow_forwarda molecular geneticist hopes to find a gene in human liver cells that codes for an important blood-clotting protein. he knows that the nucleotide sequence of a small part of the gene is gtggactgaca. briefly explain how to obtain the desired gene answerarrow_forwardSometimes, cDNAs turn out to be “chimeras”; that is, fusions of DNA copies of two different mRNAs accidentally inserted adjacently to each other in the same clone. You suspect that a cDNA clone from the nematode Caenorhabditis elegans is such a chimera because the sequence of the cDNA insert predicts a protein with two structural domains not normally observed in the same protein. How would you use the availability of the entire genomic sequence to assess if this cDNA clone is a monster or not?arrow_forward
- Define exon shuffling and explain why its occurrence might be an evolutionary advantage.arrow_forwardAs part of a project investigating potential new drug targets in the fight against malaria, you are seeking to clone the gene for a protein from the malaria parasite Plasmodium falciparum. You wish to express this protein in BL21 (DE3) cells, a standard laboratory strain of Escherichia coli. After purification of your protein, you run an SDS-PAGE gel and notice that the major band has lower molecular weight than expected, so you fear you are getting a truncated version. 1. What technique could you use to confirm that you are obtaining a shortened version of your intended protein? explainarrow_forwardAs part of a project investigating potential new drug targets in the fight against malaria, you are seeking to clone the gene for a protein from the malaria parasite Plasmodium falciparum. You wish to express this protein in BL21 (DE3) cells, a standard laboratory strain of Escherichia coli. After purification of your protein, you run an SDS-PAGE gel and notice that the major band has lower molecular weight than expected, so you fear you are getting a truncated version. (a) Give TWO possible causes of your protein becoming truncated. explainarrow_forward
- Explain why a minimum of 32 tRNAs are required to translate the “standard” genetic code.arrow_forwardExplain the reason why different parts of the mRNA is represented at different levels in an RNA-sequence analysis of mRNAs from liver.arrow_forwardSometimes, cDNAs turn out to be “chimeras”; that is, fusions of DNA copies of two different mRNAs accidentally inserted adjacently to each other in the same clone.You suspect that a cDNA clone from the nematodeCaenorhabditis elegans is such a chimera because the sequence of the cDNA insert predicts a protein with twostructural domains not normally observed in the sameprotein. How would you use the availability of the entiregenomic sequence to assess if this cDNA clone is a monster or not?arrow_forward
- Human Anatomy & Physiology (11th Edition)BiologyISBN:9780134580999Author:Elaine N. Marieb, Katja N. HoehnPublisher:PEARSONBiology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStaxAnatomy & PhysiologyBiologyISBN:9781259398629Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa StouterPublisher:Mcgraw Hill Education,
- Molecular Biology of the Cell (Sixth Edition)BiologyISBN:9780815344322Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter WalterPublisher:W. W. Norton & CompanyLaboratory Manual For Human Anatomy & PhysiologyBiologyISBN:9781260159363Author:Martin, Terry R., Prentice-craver, CynthiaPublisher:McGraw-Hill Publishing Co.Inquiry Into Life (16th Edition)BiologyISBN:9781260231700Author:Sylvia S. Mader, Michael WindelspechtPublisher:McGraw Hill Education