LIFE: THE SCIENCE OF BIOLOGY
11th Edition
ISBN: 9781319145125
Author: Sadava
Publisher: MAC HIGHER
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Chapter 18.4, Problem 3R
Summary Introduction
To review:
Both CRISPR (clustered regularly interspaced short palindromic repeat) technology and antisense ribonucleic acid (RNA) technologies are used in blocking the gene expression. Determine the advantage of using CRISPR technology over antisense RNA technology,
Introduction:
Change in deoxyribonucleic acid (DNA) sequences or any mutation causes an alteration in the proteins. The cause-and-effect relationship in proteins can be demonstrated by mutation. Alteration of protein can be stopped by inactivating specific genes. It can be done using antisense RNA and CRISPR technology.
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Consider the following human genetic diseases: hemophilia, Down syndrome, cystic fibrosis, and brain cancer. Which are the best candidates for treatment with CRISPR-Cas genome editing, and which have the largest hurdles to overcome? Why?
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 How do scientist test to ensure their gene editing experiments target only the desired specific genes?
What are the major components of the CRISPR-Cas9 system? What mechanism does it employ to combine DNA? Explain the process of how the CRISP-Cas9 system is able to create recombinant DNA. Relate the idea of gene modification to the fields of vaccines and applied microbiology as well.
Chapter 18 Solutions
LIFE: THE SCIENCE OF BIOLOGY
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- Why is genome editing by CRISPR-Cas advantageous over traditionalmethods for creating knockout or transgenic animals?Explain your answers.arrow_forwardYou are working to optimize CRISPR. How could you use ChIP-Seq to inform your design of guide RNA?arrow_forwardSite directed mutagenesis is used to: 1.determine the critical base per sequences in a Genome 2. Create genomic libraries 3. Determine the critical amino acids in a protein that allowed to function 4. Amplify dna 5. sequence dnaarrow_forward
- Briefly explain how synthetic probes are created to screen a DNA library when the protein encoded by the gene is known.arrow_forwardwhat are the advantages and disadvantages of CRISPR cas9 technology?arrow_forwardCRISPR/Cas9 can be used in genome editing. Among the following statements, which one is correct? The CRISPR and the Cas9 parts both recognise the DNA target sequence and then recruit an endonuclease for cutting it The Cas9 part recognises the DNA target sequence and the CRISPR part cuts it The CRISPR and the Cas9 parts both recognise and cut the DNA target sequence. The CRISPR part recognises the DNA target sequence and Cas9 cuts it.arrow_forward
- Which of the following statements about the CRISPR-Cas9 technique is correct? 1. Catalytically inactive Cas-9 (dCas9) cuts only one strand of DNA. 2. The guide RNA used in the lab is analogous to the tracrRNA/crRNA hybrid in the adaptive immune system of bacteria. 3. Homology directed repair has a high degree of efficiency (ie, most target regions show integration of donor DNA). 4. NHEJ can lead to mutations. O A. 1, 2 and 3 B. 1 and 3 C. 2 and 4 D. 4 only E. All of 1, 2, 3 and 4 are correct Oarrow_forwardWhich of the following is a correct statement about CRISPR-Cas-9 gene editing? Group of answer choices A single guide RNA (sgRNA) recognizes a genomic region followed by 5'-NGG-3' PAM sequence A single guide RNA (sgRNA) recognizes a genomic region followed by a long sequence palindrome repeat A single guide DNA (sgDNA) recognizes a genomic region followed by 5'-NGG-3' PAM sequence PAM sequences induce single stranded breaks that are then repaired by the CAS-9 enzymearrow_forwardWhich two molecules are necessary for every CRISPR gene editing experiment? What additional molecule is needed for gene editing using homology directed repair (HDR) that is not needed for non-homologous end joining (NHEJ)? What methods are used to introduce these molecules into the cells that are being targeted for gene editing?arrow_forward
- Describe the cloning strategies that are mediated by homopolymers tailing, linkers and adaptors. How can adaptors be employed for the creation of a novel restriction enzyme site for the introduction of the insert DNA?arrow_forwardEhat primer sets could be amplify the following DNA sequence? AATACGTCGCATGGggatccttttttatgcatgarrow_forwardPractical applications of recombinant DNA technology include the: 1. Efficient production of useful proteins and development of a new type of vaccines 2. Creation of novel genotypes for the synthesis of economically important molecules. 3. Generation of DNA and RNA sequences for use in medical diagnosis. 4. all of the above WHICH OF THE FOLLOWING TECHNIQUE IS NOT USED TO MANIPULATE THE GENOME 1. siRNA 2. ZF (zinc finger) 3. TALE (transcription activator light effector) 4. CRISPR COMMON DISEASE- COMMON VARIANT HYPOTHESIS STATES THAT 1. common disorders are likely influences by genetic variation that is also common in the population 2. if an SNP produced a deleterious mutation and changes amino acid sequence in 40% of individuals- it will produce disease phenotype in 40% individual 3. A SNP produce little change in gene expression- it will produce slight increase in risk/ manifestation of disease phenotype 4. all of the above WHAT IS TRUE REGARDING GENE ORGANIZATION IN THE NUCLEUS…arrow_forward
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