Brock Biology of Microorganisms (15th Edition)
15th Edition
ISBN: 9780134261928
Author: Michael T. Madigan, Kelly S. Bender, Daniel H. Buckley, W. Matthew Sattley, David A. Stahl
Publisher: PEARSON
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Textbook Question
Chapter 19, Problem 2AQ
You wish to know whether Archaea exist in a lake water sample but are unsuccessful in culturing any. Using techniques described in this chapter, how could you determine whether Archaea exist in the sample, and if they do, what proportion of the cells in the lake sample are Archaea?
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Chapter 19 Solutions
Brock Biology of Microorganisms (15th Edition)
Ch. 19.1 - Describe the enrichment strategy behind...Ch. 19.1 - Why is sulfate (So42) added to a Winogradsky...Ch. 19.1 - What is enrichment bias? How does dilution reduce...Ch. 19.1 - Why do the results of a direct enrichment of an...Ch. 19.2 - What is a pure culture and why is obtaining one...Ch. 19.2 - How does the agar dilution method differ from...Ch. 19.2 - What criteria serve to demonstrate that a culture...Ch. 19.3 - How might you isolate a morphologically unique...Ch. 19.3 - What is meant by high-throughput in culturing...Ch. 19.3 - What feature of high-throughput culturing relieves...
Ch. 19.4 - How does viability staining differ from stains...Ch. 19.4 - What types of environments limit the application...Ch. 19.4 - Why is it incorrect to say that the GFP is a...Ch. 19.4 - Prob. 1CRCh. 19.5 - What structure in the cell is the target for...Ch. 19.5 - FISH and CARD-FISH can be used to reveal different...Ch. 19.5 - Why is CARD-FISH more suitable than FISH for...Ch. 19.6 - What could you conclude from PCR/DGGE analysis of...Ch. 19.6 - What surprising finding has come out of many...Ch. 19.6 - How has next-generation sequencing technology...Ch. 19.6 - QWhich method, ARISA or T-RFLP, would provide more...Ch. 19.7 - Prob. 1MQCh. 19.7 - What are the advantages and disadvantages of...Ch. 19.7 - Why might a microarray be superior to using...Ch. 19.8 - Prob. 1MQCh. 19.8 - How do environmental genomic approaches differ...Ch. 19.8 - Prob. 3MQCh. 19.8 - Prob. 1CRCh. 19.9 - Prob. 1MQCh. 19.9 - If a large pulse of organic matter entered the...Ch. 19.9 - Q What are the major advantages of radioisotopic...Ch. 19.10 - What is the simplest explanation for why lunar...Ch. 19.10 - What is the expected isotopic composition of...Ch. 19.10 - How might exchange of metabolites among members of...Ch. 19.10 - Will autotrophic organisms contain more or less...Ch. 19.11 - How could NanoSIMS be used to identify a...Ch. 19.11 - Prob. 2MQCh. 19.11 - How does MAR-FISH link microbial diversity and...Ch. 19.11 - Q What can MAR-FISH tell you that FISH alone...Ch. 19.12 - How can stable isotope probing reveal the identity...Ch. 19.12 - What key method is required to do genomics on a...Ch. 19.12 - Prob. 3MQCh. 19.12 - How would you use cytometric cell sorting to...Ch. 19 - Design an experiment for measuring the activity of...Ch. 19 - You wish to know whether Archaea exist in a lake...Ch. 19 - Design an experiment to solve the following...Ch. 19 - Design a SIP experiment that would allow you to...
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- Where would you expect to find barotolerant and piezophilic bacteria? Explain your answer.arrow_forwardAna wants to engineer a super archaea which can survive and thrive in extremely hot and hypersaline water. What characteristics should she use to achieve the organism? Provide at least 3 and justify your answer.arrow_forwardWhich of the following archaea would most likely be found coexisting with bacteria?a) Nanoarchaeum b) Halobacterium c) Methanococcusd) Picrophilus e) Sulfolobusarrow_forward
- Which type of prokaryote is shown in this image? A. Photoheterotroph B. Chemoheterotroph C. Photoautotroph D. Chemoautotropharrow_forwardWhy are endospores particularly promlematic in the food industry? State two protocols the food industry has adapted to reduce endospore-forming bacteria in food prodcuts, such as chilled packaged foods, canned foods, and pasteurized milk.arrow_forwardWhich group of Archaea live in anaerobic (no oxygen) conditions such as garbage dumps? Group of answer choices Halophiles Thermophiles Methanogensarrow_forward
- Which microbe is NOT found present in water with eutrophication events(s)? A. Nitromonas sp. B. Bacteroides sp. C. Anabaena sp. D. Cyanobacteriaarrow_forwardIf you visit a lake in the middle of the Amazon and take water samples to analyze under a microscope from a nearby university that has good instrumentation and there you detect microorganisms that are not visible to the naked eye. What studies would you do to determine if they are bacteria, archaea, fungi or protozoa? Explain each type of analysis and how would you rule out members of each group? Write at least 3 paragraphsarrow_forwardDespite many interesting characteristics and features observed in Archaea, why is there not much study about these organisms?arrow_forward
- Why are archaea commonly referred to as extremophiles?arrow_forwardConsidering what you know about molds and yeasts, why do you think that we do not use the same nutrient agar used for bacteria to grow yeasts and moldsarrow_forwardEndospores : A. are a dormant form of a bacterium AND are formed by members of medically relevant groups of bacteria. B. are a form of reproduction AND are formed by members of medically relevant groups of bacteria. C. are a dormant form of a bacterium AND are involved in anaerobic respiration. D. are formed by members of medically relevant groups of bacteria AND are involved in anaerobic respiration.arrow_forward
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