Brock Biology of Microorganisms (15th Edition)
15th Edition
ISBN: 9780134261928
Author: Michael T. Madigan, Kelly S. Bender, Daniel H. Buckley, W. Matthew Sattley, David A. Stahl
Publisher: PEARSON
expand_more
expand_more
format_list_bulleted
Concept explainers
Videos
Textbook Question
Chapter 19, Problem 4AQ
Design a SIP experiment that would allow you to determine which organisms in a lake water sample were capable of oxidizing the hydrocarbon hexane (C6H14). Assume that four different species could do this. How would you combine SIP with other molecular analyses to identify these four species?
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
You have purified cytochrome C from a newly discovered species of bacteria called VCU. When you obtain the amino acid sequence you compare it to the cytochrome C amino acid sequence from other bacterial species.
Species A- 16 amino acid differences with VCU
Species B- 3 amino scid differences with VCU
Species C- 6 amino acid differences with VCU
Species D- 18 amino acid differences with VCU
List the bacterial species (A-D) from closest related to VCU to most distantly related to VCU
Compare the amount of difference in the cytochrome c amino acid sequence between human beings, chimps, rhesus monkeys, horses, chickens, bullfrogs, fruitflies, silkworms, wheat and yeast. What does it suggest about these organism's relationships to each other?
If the rate of amino acid replacement for the cytochrome c protein is 9.0E-10 replacements per amino acid site per year, and the proportion of substitutions between wooly twizzlers and hairless posquats (2 made up species) at this gene is 0.1212, how many years ago did the cytochrome c protein in wooly twizzlers and hairless posquats diverge from each other?
Chapter 19 Solutions
Brock Biology of Microorganisms (15th Edition)
Ch. 19.1 - Describe the enrichment strategy behind...Ch. 19.1 - Why is sulfate (So42) added to a Winogradsky...Ch. 19.1 - What is enrichment bias? How does dilution reduce...Ch. 19.1 - Why do the results of a direct enrichment of an...Ch. 19.2 - What is a pure culture and why is obtaining one...Ch. 19.2 - How does the agar dilution method differ from...Ch. 19.2 - What criteria serve to demonstrate that a culture...Ch. 19.3 - How might you isolate a morphologically unique...Ch. 19.3 - What is meant by high-throughput in culturing...Ch. 19.3 - What feature of high-throughput culturing relieves...
Ch. 19.4 - How does viability staining differ from stains...Ch. 19.4 - What types of environments limit the application...Ch. 19.4 - Why is it incorrect to say that the GFP is a...Ch. 19.4 - Prob. 1CRCh. 19.5 - What structure in the cell is the target for...Ch. 19.5 - FISH and CARD-FISH can be used to reveal different...Ch. 19.5 - Why is CARD-FISH more suitable than FISH for...Ch. 19.6 - What could you conclude from PCR/DGGE analysis of...Ch. 19.6 - What surprising finding has come out of many...Ch. 19.6 - How has next-generation sequencing technology...Ch. 19.6 - QWhich method, ARISA or T-RFLP, would provide more...Ch. 19.7 - Prob. 1MQCh. 19.7 - What are the advantages and disadvantages of...Ch. 19.7 - Why might a microarray be superior to using...Ch. 19.8 - Prob. 1MQCh. 19.8 - How do environmental genomic approaches differ...Ch. 19.8 - Prob. 3MQCh. 19.8 - Prob. 1CRCh. 19.9 - Prob. 1MQCh. 19.9 - If a large pulse of organic matter entered the...Ch. 19.9 - Q What are the major advantages of radioisotopic...Ch. 19.10 - What is the simplest explanation for why lunar...Ch. 19.10 - What is the expected isotopic composition of...Ch. 19.10 - How might exchange of metabolites among members of...Ch. 19.10 - Will autotrophic organisms contain more or less...Ch. 19.11 - How could NanoSIMS be used to identify a...Ch. 19.11 - Prob. 2MQCh. 19.11 - How does MAR-FISH link microbial diversity and...Ch. 19.11 - Q What can MAR-FISH tell you that FISH alone...Ch. 19.12 - How can stable isotope probing reveal the identity...Ch. 19.12 - What key method is required to do genomics on a...Ch. 19.12 - Prob. 3MQCh. 19.12 - How would you use cytometric cell sorting to...Ch. 19 - Design an experiment for measuring the activity of...Ch. 19 - You wish to know whether Archaea exist in a lake...Ch. 19 - Design an experiment to solve the following...Ch. 19 - Design a SIP experiment that would allow you to...
Additional Science Textbook Solutions
Find more solutions based on key concepts
Modified True/False 9. A giant bacterium that is large enough to be seen without a microscope is Selenomonas.
Microbiology with Diseases by Body System (4th Edition)
Define histology.
Fundamentals of Anatomy & Physiology (11th Edition)
WHAT IF? For each theme discussed in this section give an example not mentioned in the text
Campbell Biology in Focus
Define histology.
Fundamentals of Anatomy & Physiology Plus Mastering A&P with eText - Access Card Package (10th Edition) (New A&P Titles by Ric Martini and Judi Nath)
Some species of bacteria that live at the surface of sediment on the bottom of lakes are capable of using eithe...
Biology: Life on Earth with Physiology (11th Edition)
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- What does Oparin-Haldane Hypothesis assume about Earth’s primordial atmosphere? Why would this have been important for the origin of biologically significant molecules?arrow_forwardWhat evidence is there for the hypothesis that life originated on Earth by the creation and polymerization of small organic molecules by natural processes?arrow_forwardWhich of the following was the least likely objective of the Urey-Miller experiment? Group of answer choices To demonstrate that oxygen can be produced by plants. To recreate the conditions of the Earth before life existed and produce the molecules necessary for life from primordial inorganic matter (water, methane, ammonia, and carbon dioxide). To build a case (though not necessarily prove) that life can originate from nonliving matter through natural means. To demonstrate that organic molecules like amino acids (building blocks of protein) and nucleotide bases (building blocks of DNA) do not necessarily originate only from living matter. To demonstrate that lightning may have been the essential energy source (early in Earth's evolution) in the production of molecules essential for life.arrow_forward
- Dichotomous Keys are used to identify an organism based on a choice between character states. Can you answer all the parts to this question please in complete sentences Based on the name, how many options should you have when working with a dichotomous key? Why does the term “dichotomous” let you know that? (hint: look up the Greek roots and meanings for this word)arrow_forwardWhich of the following questions about the origin of life on Earth is most scientifically testable? * Why were the earliest life-forms created using only twenty amino acids? Did life originate to make the universe a better place? Was catalytic RNA used by ancient organisms as a stepping stone to acquire protein enzymes? Can simple organic molecules form spontaneously in an oxygen-free atmosphere?arrow_forwardAn analysis of an octopus’s DNA determines that the nitrogen base thymine makes up 18 percent of the genome. Which of the following best represents the percent of guanine Chargaff would have found in the octopus’s genome?arrow_forward
- Isoprene serves as a building block not only for the hydrocarbons observed in archaeal membranes but also for sterols, carotenoids, retinal, and quinones. Use any resources necessary to identify the function of these other isoprene-based molecules and to determine their distribution in nature. What does the use of isoprene to make this diverse array of molecules suggest about the nature of the last universal common ancestor (LUCA)?arrow_forwardWas there molecular oxygen in the earth's primitive atmosphere? How has that molecule become abundant?arrow_forwardMany scientists claim that the synthesis of the first organic molecules from inorganic precursors was possible because of the highly reducing atmosphere found on primitive Earth. Which of the following is an appropriate null hypothesis that could be used when investigating the claim? * The level of atmospheric oxygen on modern Earth is significantly higher than on primitive Earth. Organic molecules were transported to primitive Earth by a meteorite or other celestial event. The synthesis of organic molecules from inorganic molecules is possible under current atmospheric oxygen levels as well as those found on primitive Earth. The absence of a significant quantity of atmospheric oxygen is required for the synthesis of organic molecules from inorganic precursors.arrow_forward
- If the same portion of the amino acid sequence for turkey hemoglobin was included, who do you think it would have the least differences with, the human, gorilla,horse,kangaroo, or chicken? Why do you think so?arrow_forwardA researcher studying two species (species 1 and species 2) sequences a short stretch of eight codons from the same gene, gene B, in each and compares them. Species 1 and species 2 had a most recent common ancestor 50 million years ago. Species 1: ATC GGG CGG GAC TTA CTA TAT GCC Species 2: ATT GGG CGG GAC TTG CTA TAT GCC Given the differences between the sequences of the two species' genes shown here, what evolutionary force can you predict is most likely in operation on gene B?arrow_forwardBy comparing DNA sequence of a specific gene, we can determine the evolutionary relationship of two organisms. Based on the sequence listed below, which two species would you expect to be more closely related? Organism No.1 with ATG CAA TAC GCC, organism No. 2 with ATG CAT GAC ACC and organims No. 3 with ATG CAT TAC GCC A. Organims No.2 and No.3 are likely more closely related. b.Organims No.1 and No.2 are likely more closely related. c.Organims No 1 and No.3 are likely more closely related.arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Biology Today and Tomorrow without Physiology (Mi...BiologyISBN:9781305117396Author:Cecie Starr, Christine Evers, Lisa StarrPublisher:Cengage Learning
Biology Today and Tomorrow without Physiology (Mi...
Biology
ISBN:9781305117396
Author:Cecie Starr, Christine Evers, Lisa Starr
Publisher:Cengage Learning
Mechanisms of Genetic Change or Evolution; Author: Scientist Cindy;https://www.youtube.com/watch?v=5FE8WvGzS4Q;License: Standard Youtube License