Biology - Study Guide
8th Edition
ISBN: 9780321501561
Author: Martha R. Taylor
Publisher: PEARSON EDUCATION (COLLEGE)
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Chapter 19, Problem 8TYK
Summary Introduction
Introduction: A virus is an infectious agent that cannot be seen through the naked eyes. Virology is the study of viruses and virologists are the biologists who study viruses. Viruses are obligate intracellular
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a) what are restriction enzymes?
b) What is the main function of restriction enzymes in nature?
c) Compare and contrast the these enzymes in nature and in scientific research.
The activity of restriction enzymes may produce fragments with sticky ends. Sticky ends are
a) a type of endonucleases.
b) dephosphorylated CpG islands.
c) unpaired nucleotides.
d) double breaks with blunt ends.
Regarding the PCR technique, what is false?a. It can produce multiple copies of DNA.b. It is the same as DNA fingerprinting.c. It is not a time-consuming process.d. It cannot successfully copy whole genes
Chapter 19 Solutions
Biology - Study Guide
Ch. 19 - In the following diagram of lytic and lysogenic...Ch. 19 - Summarize the flow of genetic information during...Ch. 19 - How does a virus spread throughout a plant? Are...Ch. 19 - Create a concept map that describes the lytic and...Ch. 19 - Prob. 2SYKCh. 19 - Prob. 1TYKCh. 19 - Prob. 2TYKCh. 19 - Prob. 3TYKCh. 19 - Prob. 4TYKCh. 19 - T2 and T4 are two types of bacteriophage that...
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- PCR can be used ______. a. as a cloning vector b. in DNA profiling c. to modify a human genomearrow_forwardPut the following tasks in the order they would occur during a DNA cloning experiment. a. using DNA ligase to seal DNA fragments into vectors b. using a probe to identify a clone in the library c. sequencing the DNA of the clone d. making a DNA library of clones e. cutting genomic DNA with restriction enzymesarrow_forwardCloning Genes Is a Multistep Process In cloning human DNA, why is it necessary to insert the DNA into a vector such as a bacterial plasmid?arrow_forward
- Which technique rapidly replicates specific DNA fragments without cloning in cells? (a) gel electrophoresis (b) cDNA libraries (c) DNA probe (d) restriction fragment length polymorphism (e) polymerase chain reactionarrow_forwardWhat is a cloning vector? A. The DNA probe used to locate a particular gene in the genome. B. An agent such as plasmid, used to transfer DNA from an in vitro solution into a living cell. C. The laboratory apparatus used to clone genes. D. An enzyme that cuts DNA into restriction fragments.arrow_forwardWhat are the sticky ends of the restriction fragments? Select one: a. The surfaces of sticky ends contain matching base pairs, allowing fragments to splice. b. The surfaces of sticky ends have glue like substance that allow fragments to splice. c. The surfaces of sticky ends contain the exact same nucleotides, allowing fragments to bond. d. The surfaces of sticky ends have velcro like structure, allowing fragments to bond.arrow_forward
- Which of the following would you need to clone a human gene? Choose all that apply a) A surrogate mother b) A plasmid vector (or other type of DNA vector, e.g. phage, BAC) c) A donor goat nucleus d) A restriction enzyme e) Human DNA f) An enucleated egg g) DNA ligasearrow_forwardIn 5 sentences only, What are restriction enzymes (RE)? Describe how a RE can be used to develop/design a DNAmarker.arrow_forwardRestriction enzymes (type II) bind to their recognition site and A. begin elongation from it B. methylaet it C. cut inside it D. ligate itarrow_forward
- Using the data in Table, identify restriction enzymes that (a) produce blunt ends; (b) recognize and cleave the same sequence (called isoschizomers); (c) produce identical sticky ends.arrow_forwardFollowing are four processes common to most cloning experiments: a) transforming bacteria b) plating bacteria on selective medium c) cutting DNA with restriction endonucleases d) ligating DNA fragments Place components of this list in the order in which they would most likely occur during a cloning experiment.arrow_forwardExplain briefly within 5 sentences each 1. a. What is PCR? Explain/relate its importance in DNA marker analysis. 1.b. What are restriction enzymes (RE)? Describe how a RE can be used to develop/design a DNAmarker.arrow_forward
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