Brock Biology of Microorganisms
15th Edition
ISBN: 9780134626352
Author: MADIGAN
Publisher: PEARSON
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Chapter 19.9, Problem 1CR
Q What are the major advantages of radioisotopic methods in the study of microbial ecology? What type of controls (discuss at least two) would you include in a radioisotopic experiment to show 14CO2 incorporation by phototrophic bacteria or to show
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Bacteria multiply exponentially. How can this pattern assist us in calculating bacterial growth? Can calculations assist us in understanding microbial development, and if yes, what does it assist us in understanding? How would you use these equations in real-world situations? Can you give an example of when learning how to quantify microbial growth might come in handy?
state 2 advantages and two disadvantages of using ; radioisotopic and stable isotopic methods in the study of microbial ecology.
You can determine the Z-value for both organisms by calculating the temperature change required to reduce the D value by 1 log cycle.
(Observations: Notice the difference in the two organisms. One has a steeper downward slope, but is offset higher. It is slightly more heat-tolerant, but slightly more susceptible to increases in temperature.)
Question: Raw milk can be contaminated by several common pathogenic bacteria (i.e. Staphylococcus, M. tuberculosis) and therefore must be pasteurized. Your goal is to find the minimum temperature and time required to destroy these organisms. If you know the Z-values for staphylococcus and M. tuberculosis, do you have enough information to set the appropriate heating parameters?
Chapter 19 Solutions
Brock Biology of Microorganisms
Ch. 19.1 - Describe the enrichment strategy behind...Ch. 19.1 - Why is sulfate (So42) added to a Winogradsky...Ch. 19.1 - What is enrichment bias? How does dilution reduce...Ch. 19.1 - Why do the results of a direct enrichment of an...Ch. 19.2 - What is a pure culture and why is obtaining one...Ch. 19.2 - How does the agar dilution method differ from...Ch. 19.2 - What criteria serve to demonstrate that a culture...Ch. 19.3 - How might you isolate a morphologically unique...Ch. 19.3 - What is meant by high-throughput in culturing...Ch. 19.3 - What feature of high-throughput culturing relieves...
Ch. 19.4 - How does viability staining differ from stains...Ch. 19.4 - What types of environments limit the application...Ch. 19.4 - Why is it incorrect to say that the GFP is a...Ch. 19.4 - Prob. 1CRCh. 19.5 - What structure in the cell is the target for...Ch. 19.5 - FISH and CARD-FISH can be used to reveal different...Ch. 19.5 - Why is CARD-FISH more suitable than FISH for...Ch. 19.6 - What could you conclude from PCR/DGGE analysis of...Ch. 19.6 - What surprising finding has come out of many...Ch. 19.6 - How has next-generation sequencing technology...Ch. 19.6 - QWhich method, ARISA or T-RFLP, would provide more...Ch. 19.7 - Prob. 1MQCh. 19.7 - What are the advantages and disadvantages of...Ch. 19.7 - Why might a microarray be superior to using...Ch. 19.8 - Prob. 1MQCh. 19.8 - How do environmental genomic approaches differ...Ch. 19.8 - Prob. 3MQCh. 19.8 - Prob. 1CRCh. 19.9 - Prob. 1MQCh. 19.9 - If a large pulse of organic matter entered the...Ch. 19.9 - Q What are the major advantages of radioisotopic...Ch. 19.10 - What is the simplest explanation for why lunar...Ch. 19.10 - What is the expected isotopic composition of...Ch. 19.10 - How might exchange of metabolites among members of...Ch. 19.10 - Will autotrophic organisms contain more or less...Ch. 19.11 - How could NanoSIMS be used to identify a...Ch. 19.11 - Prob. 2MQCh. 19.11 - How does MAR-FISH link microbial diversity and...Ch. 19.11 - Q What can MAR-FISH tell you that FISH alone...Ch. 19.12 - How can stable isotope probing reveal the identity...Ch. 19.12 - What key method is required to do genomics on a...Ch. 19.12 - Prob. 3MQCh. 19.12 - How would you use cytometric cell sorting to...Ch. 19 - Design an experiment for measuring the activity of...Ch. 19 - You wish to know whether Archaea exist in a lake...Ch. 19 - Design an experiment to solve the following...Ch. 19 - Design a SIP experiment that would allow you to...
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