Brock Biology of Microorganisms
15th Edition
ISBN: 9780134626352
Author: MADIGAN
Publisher: PEARSON
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Chapter 19, Problem 3AQ
Design an experiment to solve the following problem: Determine the rate of methanogenesis (CO2 + 4 H2 → CH4 + 2 H2O) in anoxic lake sediments and whether or not it is H2-limited. Also, determine the morphology of the dominant methanogen (recall that these are Archaea, 
Section 17.2). Finally, calculate what percentage the dominant methanogen is of the total archaeal and total prokaryotic populations in the sediments. Remember to specify necessary controls.
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Chapter 19 Solutions
Brock Biology of Microorganisms
Ch. 19.1 - Describe the enrichment strategy behind...Ch. 19.1 - Why is sulfate (So42) added to a Winogradsky...Ch. 19.1 - What is enrichment bias? How does dilution reduce...Ch. 19.1 - Why do the results of a direct enrichment of an...Ch. 19.2 - What is a pure culture and why is obtaining one...Ch. 19.2 - How does the agar dilution method differ from...Ch. 19.2 - What criteria serve to demonstrate that a culture...Ch. 19.3 - How might you isolate a morphologically unique...Ch. 19.3 - What is meant by high-throughput in culturing...Ch. 19.3 - What feature of high-throughput culturing relieves...
Ch. 19.4 - How does viability staining differ from stains...Ch. 19.4 - What types of environments limit the application...Ch. 19.4 - Why is it incorrect to say that the GFP is a...Ch. 19.4 - Prob. 1CRCh. 19.5 - What structure in the cell is the target for...Ch. 19.5 - FISH and CARD-FISH can be used to reveal different...Ch. 19.5 - Why is CARD-FISH more suitable than FISH for...Ch. 19.6 - What could you conclude from PCR/DGGE analysis of...Ch. 19.6 - What surprising finding has come out of many...Ch. 19.6 - How has next-generation sequencing technology...Ch. 19.6 - QWhich method, ARISA or T-RFLP, would provide more...Ch. 19.7 - Prob. 1MQCh. 19.7 - What are the advantages and disadvantages of...Ch. 19.7 - Why might a microarray be superior to using...Ch. 19.8 - Prob. 1MQCh. 19.8 - How do environmental genomic approaches differ...Ch. 19.8 - Prob. 3MQCh. 19.8 - Prob. 1CRCh. 19.9 - Prob. 1MQCh. 19.9 - If a large pulse of organic matter entered the...Ch. 19.9 - Q What are the major advantages of radioisotopic...Ch. 19.10 - What is the simplest explanation for why lunar...Ch. 19.10 - What is the expected isotopic composition of...Ch. 19.10 - How might exchange of metabolites among members of...Ch. 19.10 - Will autotrophic organisms contain more or less...Ch. 19.11 - How could NanoSIMS be used to identify a...Ch. 19.11 - Prob. 2MQCh. 19.11 - How does MAR-FISH link microbial diversity and...Ch. 19.11 - Q What can MAR-FISH tell you that FISH alone...Ch. 19.12 - How can stable isotope probing reveal the identity...Ch. 19.12 - What key method is required to do genomics on a...Ch. 19.12 - Prob. 3MQCh. 19.12 - How would you use cytometric cell sorting to...Ch. 19 - Design an experiment for measuring the activity of...Ch. 19 - You wish to know whether Archaea exist in a lake...Ch. 19 - Design an experiment to solve the following...Ch. 19 - Design a SIP experiment that would allow you to...
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- Methanopyrus kandleri and Pyrolobus fumarij are bacteria that live near hydrothermal vents under the oceans. Temperatures at these vents can reach well over 100 degrees Celsius. Both organisms obtain energy from hydrogen but Methanopyrus kandleri grows best at 98 degrees Celsius while Pyrolobus fumarij prefers to reproduce at temperatures above 100 degrees Celsius. These bacteria provide the base of a food web at the bottom of the ocean floor. What questions could a student ask in order to investigate this difference between the bacteria? A. What kind of heat tolerance do other nearby organisms have? B. Does the Methanopyrus kandleri live farther away from the vent than the Pyrolobus fumarij? C. Does Methanopyrus kandleri have a thicker cell wall than Pyrolobus fumarij? D. Is one organism better adapted to the environment than the other?arrow_forwardThere are three main ways that bacteria and archaea are essential to eukaryotic life. Explain the three different ways and give at least one example of each.arrow_forwardWhich of the following statements is true? A. Archaea and bacteria have identical membrane lipids. B. The cell walls of archaea lack peptidoglycan. C. Prokaryotes have low levels of genetic diversity. D. No archaea are capable of using CO2 to oxidize H2 releasing methane.arrow_forward
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